Expression and Immunoreactivities of Hepatitis E Virus Genotype 3 Open Reading Frame-2 (ORF-2) Recombinant Proteins Expressed in Insect Cells

Hepatitis E virus (HEV) is a fecal-orally transmitted virus that is endemic in many geographical areas with poor sanitary conditions and inadequate water supplies. In Europe, a low-endemic area, an increased number of autochthonous sporadic human cases of patients infected with HEV strains of genotype 3, have been reported lately. The relatively high prevalence of HEV genotype 3 infections in European pigs has raised concerns about a potential zoonotic transmission to humans. Determination of HEV seroprevalence in pigs would help to clarify its incidence and possible zoonotic implications. To this purpose, we have expressed and partially characterized swine genotype 3 HEV open reading frame-2 proteins upon infection of Sf21 insect cells with recombinant baculoviruses. The use of the expressed proteins as diagnostic antigens for the detection of antibodies to HEV has been further assayed with human and swine sera.     

顺序氯化消毒工艺的研究

研究了短时游离氯后转氯胺的顺序消毒工艺.结果表明,该消毒工艺对指示微生物的灭活效果优于游离氯消毒,游离氯和氯胺存在协同消毒作用;对脊髓灰质炎病毒和大肠杆菌f₂噬菌体的灭活效果与游离氯相同.相同原水条件下,顺序氯化消毒工艺产生的三卤甲烷浓度比游离氯消毒工艺减少35.8%～77.0%;卤乙酸减少36.6%～54.8%.消毒进水水质越差,顺序氯化消毒工艺在消毒副产物(DBP)控制方面就越具优势.     

Who should wear mask against airborne infections? Altering the contact network for controlling the spread of contagious diseases

There are several ways of controlling the propagation of a contagious disease. For instance, to reduce the spreading of an airborne infection, individuals can be encouraged to remain in their homes and/or to wear face masks outside their domiciles. However, when a limited amount of masks is available, who should use them: the susceptible subjects, the infective persons or both populations? Here we employ susceptible-infective-recovered (SIR) models described in terms of ordinary differential equations and probabilistic cellular automata in order to investigate how the deletion of links in the random complex network representing the social contacts among individuals affects the dynamics of a contagious disease. The inspiration for this study comes from recent discussions about the impact of measures usually recommended by health public organizations for preventing the propagation of the swine influenza A (H1N1) virus. Our answer to this question can be valid for other eco-epidemiological systems.     

虚拟蜜罐技术在网络安全中的应用研究

面对网络上复杂而多变的黑客攻击,提出利用虚拟蜜罐技术主动吸引黑客的攻击,来监视和跟踪入侵者的行为并进行记录,进而研究入侵者所使用的攻击工具、攻击策略和方法。笔者论述了虚拟蜜罐技术的基本概念,通过3方面的应用实例,较为系统地研究了虚拟蜜罐技术在网络安全中的应用,证明虚拟蜜罐技术可以有效收集攻击信息,保护实际工作网络,并讨论了虚拟蜜罐的优点和风险。     

Intergrated approach for microbiological impact assessment of public wastewater treatment plants

Wastewaters are a source of pathogenic micro-organisms in the environment. The microbial load and residues found in the final effluents of wastewater-treatment plants (WWTPs) depend on the WWTPs' abatement capacity and the final disinfection treatment systems applied to wastewaters before discharge into water. A historical database with data on surface and marine-coastal water quality and on the characterization of WWTP effluents was made using data from 1997 to 2004 to assess the microbiologic impact along the coast of the Venice province (Italy, northern Adriatic sea). The monitoring of river and sea discharges along the coast is integrated with the application of the Synthetic Pluriennal Faecalization Index (ISPF). The experimental study was conducted in the period from November 2002 to April 2004 by the Veneto Regional Environmental Prevention and Protection Agency. The results of this investigation on faecal contamination together with previous data are presented with a preliminary performance characterization of the WWTPs' disinfection technologies (sodium hypochlorite, peracetic acid, UV rays, and ozone).     

Increased Infectious Disease Susceptibility Resulting from Outbreeding Depression

Abstract:  The mechanisms by which outbreeding depression leads to reduced fitness are poorly understood. We considered the hypothesis that outbreeding can depress fitness by increasing the susceptibility of hybrid individuals and populations to infectious disease. Competitive breeding trials in experimental ponds indicated that outbred largemouth bass (  Micropterus salmoides ) crossed from two geographically and genetically distinct populations suffered a reduction in fitness of approximately 14% relative to parental stocks. We measured the comparative susceptibility of these same outbred stocks to a novel viral pathogen, largemouth bass virus. Following experimental inoculation, F2 generation hybrids suffered mortality at a rate 3.6 times higher than either F1 generation hybrids or wild-type parental fish. Analysis of viral loads indicated that viral replication was more rapid in F2 fish than in F1 hybrids or wild-type parental fish. We attribute these results to the disruption of coadapted gene complexes in the immune systems of outbred fish in the F2 generation. Increased susceptibility to infectious disease may be an important but underappreciated mechanism by which outbreeding reduces the fitness of individuals and populations and by which novel infectious diseases emerge in populations of hybrid organisms.     

环境对阿尔茨海默病病因影响

阿尔茨海默病是一种发生于老年人群的原发生退行性脑病。目前，关于ＡＤ病研究中的中心问题是弄清楚其发病的病理，如果可以抑制的一步和几步，将减缓或防止其发病，因此搞清ＡＤ病的发病机理对预防和治疗ＡＤ病相当重要。     

基于社会燃烧理论的突发公共卫生事件网络情绪传播模型

为有效引导和管控突发公共卫生事件网络舆情,考虑社会相关性和网络群体间情绪状态转移的不确定性,基于社会燃烧理论研究突发公共卫生事件网络情绪传播机制。首先基于社会燃烧理论分析网络用户群体的社会影响因素,建立未燃-阴燃-燃烧-抑燃-稳定(UDBFS)网络情绪传播模型和考虑干预措施的网络情绪传播模型;然后以新冠肺炎(COVID-19)疫情为例,开展模拟仿真与干预试验,验证模型和干预措施的合理性,分析干预措施强度对网络情绪传播的影响。研究结果表明:模型便于描述新冠肺炎疫情网络情绪传播过程,干预措施能有效抑制不良网络情绪的传播,并使燃烧用户群体数量下降32.9%;同时降低"社会点火温度",可迅速减少事件爆发后短时间内"燃烧"用户群体数量。     

云南省环境监测中心站网络安全对策及应用

根据近年国内网络领域的新技术,结合本单位网络建设的需求及工作经验,从防火墙、入侵检测、防病毒、漏洞扫描、访问控制等几个方面作了阐述,对如何建设一个安全的网络提出了自己的见解。