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81.
Combined fluorescence and electrochemical investigation on the binding
interaction between organic acid and human serum albumin 总被引:1,自引:1,他引:0
Human serum albumin (HSA) is a plasma protein responsible for the binding and transport of fatty acids and a variety of exogenous chemicals such as drugs and environmental pollutants. Such binding plays a crucial role in determining the ADME (absorption, distribution, metabolism, and excretion) and bioavailability of the pollutants. We report investigation on the binding interaction between HSA and acetic acid (C2), octanoic acid (C8) and dodecanoic acid (C12) by the combination of site-specific fluorescent probe, tryptophan intrinsic fluorescence and tyrosine electrochemistry. Two fluorescent probes, dansylamide and dansyl-L-proline, were employed in the displacement measurement to study fatty acid interaction with the two drug-binding sites on HSA. Intrinsic fluorescence of tryptophan in HSA was monitored upon addition of the fatty acids into HSA. Electrocatalyzed response of the tyrosine residues in HSA by a redox mediator was used to investigate the binding interaction. Qualitatively, observations made by the three approaches are very similar. HSA did not show any change in either fluorescence or electrochemistry after mixing with C2, suggesting there is no significant interaction with the short-chain fatty acid. For C8, the measured signal dropped in a single-exponential fashion, indicative of independent and non-cooperative binding. The calculated association constant and binding ratio is 3.1×106 L/mol and 1 with drug binding Site I, 1.1×107 L/mol and 1 with Site II, and 7.0×104 L/mol and 4 with the tryptophan site. The measurement with C12 displayed multiple phases of fluorescence change, suggesting cooperativity and allosteric effect of C12 binding. These results correlate well with those obtained by the established methods, and validate the new approach as a viable tool to study the interactions of environmental pollutants with biological molecules. 相似文献
82.
基于δ18O的青藏高原中部错那湖湖水蒸发研究 总被引:2,自引:1,他引:1
稳定同位素方法经常被用来估算湖水水量平衡,这种方法主要依赖于水样的采集和同位素分析,并结合研究区域相关的气候和水文信息对湖水进行研究。基于对1999-2001年期间获得的18个湖水样的稳定同位素分析,发现错那湖水δD和δ18O的平均值分别为-83.3‰和-9.6‰,显著高于流域内同期降水中δD和δ18O的加权平均值-124.5‰和-16.6‰;流域内湖水稳定同位素的变化幅度远小于降水,但展示了明显的季节变化,夏季值较低而冬春季值较高,这种变化表现出一定的降水量效应,表明降水是湖水主要补给来源之一。结合所获得的流域内降水和河水稳定同位素数据,利用稳定同位素质量平衡方法对非结冰期间错那湖水的蒸发与流入比率(E/I)进行了估算,结果表明,受太阳辐射与气温升高的影响,湖水受到强烈的蒸发,流入错那湖的水大约24%消耗于蒸发。 相似文献
83.
A novel biphenyl-degrading bacterial strain LA-4 was isolated from activated sludge. It was identified as Dyella ginsengisoli according to phylogenetic similarity of 16S rRNA gene sequence. This isolate could utilize biphenyl as sole source of carbon and energy, which degraded over 95 mg/L biphenyl within 36 h. The major metabolites formed from biphenyl, such as 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) and benzoic acid, were identified by LC-MS. The crude cell extract of strain LA-4 exhibited the activity of 2,3-dihydroxybiphenyl 1,2-dioxygenase (2,3-DHBD) and the kinetic parameters were Km= 26.48 μmol/L and Vmax= 8.12 μmol/mg protein. A conserved region of the biphenyl dioxygenase gene bphA1 of strain LA-4 was amplified by PCR and confirmed by DNA sequencing. 相似文献
84.
Development and application of a two-dimensional water quality model for the
Daqinghe River Mouth of the Dianchi Lake 总被引:1,自引:1,他引:0
Water quality models are important tools to support the optimization of aquatic ecosystem rehabilitation programs and assess their efficiency. Basing on the flow conditions of the Daqinghe River Mouth of the Dianchi Lake, China, two-dimensional water quality model was developed in the research. The hydrodynamics module was numerically solved by the alternating direction iteration (ADI) method. The parameters of the water quality module were obtained through the in situ experiments and the lab analyses that were conducted from 2006 to 2007. The model was calibrated and verified by the observation data in 2007. Among the four modelled key variables that were water level, CODcr, NH3-N and PO4-P, the minimum value of the coefficient of determination (CoD) was 0.69, which mean the model performed reasonably well. The developed model was then applied to simulate the water quality changes at the downstream cross-section assuming that the designed restoration programs were implemented. According to the simulated results, the restoration programs could cut down the loads of CODcr and PO4-P about 15%, however, they would have very little effect on the NH3-N removal. Besides, the water quality at the outlet cross-section would be still in class V (3838-02), indicating more measures should be taken to further reduce the loads. The study demonstrated the capability of water quality models to support aquatic ecosystem restorations. 相似文献
85.
化学强化一级处理-人工湿地处理生活污水效果 总被引:6,自引:1,他引:5
为解决传统的污水二级生化处理工艺运行成本较高问题并满足日趋增长的小城镇污水处理实际需要,开展了粉煤灰基混凝剂化学强化一级处理—潜流式人工湿地(CEPT-SFCW)系统处理生活污水的实验研究。结果表明,在人工湿地负荷率为0.05~0.1m3/(m·2d)的条件下,CEPT-SFCW系统对COD的去除率>85%。同时,CEPT-SFCW系统对总磷的去除率很高,虽然在进水含磷量低时人工湿地会发生磷释放的现象,出水中磷浓度仍可达到GB8978-1996中的二级排放标准。该系统对氨氮去除效率较低,最高可达到10%,去除效果与植物的生长状态有关。 相似文献
86.
87.
88.
富营养化水体中微囊藻毒素(MCs)去除技术研究进展 总被引:2,自引:1,他引:1
当前,随着工业的发展水体富营养化程度日益严重。引起富营养化的主要藻类一蓝藻,能够释放对人体及鱼类具有多器官毒性、遗传性和致癌性的毒素——微囊藻毒素(MCs)。MCs治理技术种类繁多,传统的物理法如:混凝沉淀、膜过滤、活性炭吸附、气浮以及直接过滤只能对简单的处理细胞内的MCs;化学氧化法、光催化氧化法以及高级氧化法虽然能有效处理水体中的MCs.但有成本高、易二次污染和操作复杂等缺点,难以满足日益严格的环保要求;生物酶法除了具有生物降解法所具有的廉价、无二次污染、降解彻底和易操作等特点外,还被刺用来监控水体中MCs,它将成为今后重点研究的技术领域. 相似文献
89.
采用滇池流域5个站点的气象资料对滇池流域气候特征进行统计分析.滇池流域冬季最低温度不低于7℃,夏季最高温度不超过20℃,冬夏温差较小,适于居住.滇池流域内,滇池沿岸的站点冬夏温差相对远离流域的其它站点要小.近40多a来,滇池流域冬、夏及年气温呈现年际振荡的变化分布,滇池流域气温呈现出明显的上升趋势,且冬季增温最为明显.滇池流域的风向全年均以西南风为主,夏季滇池流域南、北部还受南风的影响,其西部受东风的影响,而东、东北部分别受东南、东北风的影响.滇池流域雨季主要从5月开始,夏季和年降水量较大的昆明、嵩明站均位于滇池流域主要风向-西南风的下风方向. 相似文献
90.
杜尔基地区处于大兴安岭中南段,区内中生代侵入岩广泛发育.八个样品K-Ar全岩法测年结果为100.15~120.29 Ma,形成于早白垩世.岩石化学分析结果具有高硅、高钾、富碱、过铝、贫铁、镁、钙特征,属于碱性岩系列.稀土元素总量平均值为146.85 μg/g,球粒陨石标准化曲线整体平缓右倾,显示富Rb,贫Sr、Ba特征,具有Eu负异常,微量元素具有明显的Sr、Ba、Ti负异常,反映了具有强烈分异的分离结晶作用存在,说明岩石形成于长期较稳定的构造背景,可能形成于非造山板内裂谷或热点地幔柱环境. 相似文献