Quantification of human amniotic fluid constituents by high resolution proton nuclear magnetic resonance (NMR) spectroscopy

We have investigated the ability of high-resolution proton NMR spectroscopy to provide a biochemical constituent screening of human amniotic fluid (AF). Proton NMR spectra were obtained at 300 MHz on AF from patients undergoing amniocentesis in the mid-trimester. Only AF from normal pregnancies (normal fetal karyotype, normal a-fetoprotein levels, normal birth outcome) was used in this study. The AF supernatant was lyophilized and resuspended in deuterated water containing 0.1 mm phosphate buffer and 6.02 mm disodium maleate. Identification of low molecular weight compounds was confirmed by two-dimensional NMR spectra (primarily correlated spectroscopy, or COSY) and standard addition techniques. A broad profile of compounds were ‘NMR visible’ in a single proton spectrum, including creatinine, glucose, organic acids (acetate, citrate, and lactate) and several amino acids (alanine, histidine, leucine, phenylalanine, tyrosine and valine). The proton spectrum was unaffected by prior freezing/thawing of AF samples. We were able to quantify compounds by comparison with an added concentration standard (maleate) at concentrations as low as 30 μm. Good agreement with literature values based on other analytical techniques was obtained.     

A Multi‐Scale Analysis of Single‐Family Residential Water Use in the Phoenix Metropolitan Area

Studies that evaluate determinants of residential water demand typically use data from a single spatial scale. Although household‐scale data are preferred, especially when econometric models are used, researchers may be limited to aggregate data. There is little, if any, empirical analysis to assess whether spatial scale may lead to ecological fallacy problems in residential water use research. Using linear mixed‐effects models, we compare the results for the relationship of single‐family water use with its determinants using data from the household and census tract scales in the city of Phoenix. Model results between the household and census tract scale are similar suggesting the ecological fallacy may not be significant. Common significant determinants on these two spatial scales include household size, household income, house age, pool size, irrigable lot size, precipitation, and temperature. We also use city/town scale data from the Phoenix metropolitan area to parameterize the linear mixed‐effects model. The difference in the parameter estimates of those common variables compared to the first two scales indicates there is spatial heterogeneity in the relationship between single‐family water use and its determinants among cities and towns. The negative relationship between single‐family house density and residential water use suggests that residential water consumption could be reduced through coordination of land use planning and water demand management.     

Toward a high-rate enhanced biological phosphorus removal process in a membrane-assisted bioreactor.

A membrane enhanced biological phosphorus removal (MEBPR) process was studied to determine the impact of hydraulic retention time (HRT) and solids retention time (SRT) on the removal of chemical oxygen demand (COD), nitrogen, and phosphorus from municipal wastewater. The MEBPR process was capable of delivering complete nitrification independent of the prevailing operating conditions, whereas a significant improvement in COD removal efficiency was observed at longer SRTs. In the absence of carbon-limiting conditions, the MEBPR process was able to achieve low phosphorus concentrations in the effluent at increasingly higher hydraulic loads, with the lowest HRT being 5 hours. The MEBPR process was also able to maintain optimal phosphorus removal when the SRT was increased from 12 to 20 days. However, at higher suspended solids concentrations, a substantial increase was observed in carbon utilization per unit mass of phosphorus removed from the influent. These results offer critical insights to the application of membrane technology for biological nutrient removal systems.     

Dairy manure treatment, digestion and nutrient recovery as a phosphate fertilizer

A combined approach of biological treatment, solids digestion and nutrient recovery was tested on dairy manure. A sequencing batch reactor (SBR) was operated in three modes, in order to optimize nutrient (nitrogen and phosphorus) removals. The highest average removal efficiencies of 91% for NH4-N, 59% for PO4-P and 80% for total chemical oxygen demand (COD) were achieved. Staining experiments suggested the coexistence of glycogen and phosphorus accumulating organisms. Anaerobic digestion of wasted bio-solids was able to produce a PO4-P concentration of 70 mgL-1 in the supernatant. A pilot-scale experiment, designed to recover phosphorus in the supernatant as struvite (magnesium ammonium phosphate), was able to remove 82% of soluble PO4-P.     

Is small hydropower beautiful? Social impacts of river fragmentation in China’s Red River Basin

Small hydropower (SHP) is promoted as a pro-poor renewable energy source that does not have the negative social impacts of large dams. This article challenges these claims, using data from a household survey in China’s upper Red River Basin. We find that SHP can fragment river systems in ways that reduce irrigation water availability, provoke changes to agricultural practices, and negatively impact river health. These social impacts of river fragmentation mainly occur in villages situated between a plant’s intake and outflow. The frequency of plant water diversions due to continued generation in the dry season significantly predicts all social impacts; installed capacity of the plant and the quality of the village’s irrigation infrastructure predict some impacts. Villages with strong local governance can negotiate with the plant to temporarily halt generation when irrigation water is needed, lessening social impacts. Our findings reveal that SHP plants are not as benign as they are made out to be; they must be built and managed according to community needs.     

Divergent perspectives on water resource sustainability in a public–policy–science context

Diverging perspectives toward environmental problems, their causes, and solutions can exacerbate controversy in participatory decision making. Past research has examined the lay–expert divide in perceptions about diverse risks, but relatively few studies have examined multidimensional perspectives on water scarcity across expert groups with different knowledge systems. We address this gap by examining conflicting perspectives across ‘lay’ residents and academic and policymaking ‘experts’ in Phoenix, AZ. We analyze ecological concern about water issues, risk perceptions regarding the factors contributing to scarcity, and policy attitudes pertaining to resource management alternatives. All three groups expressed substantial concern for broad-scale water issues, especially drought. Residents exhibited a heightened tendency to blame other people for water scarcity, in addition to opposition toward stringent approaches such as water pricing. While strongly supporting the acquisition of more supplies, policymakers exhibited lower concern about regional water use rates while displacing blame away from anthropogenic causes compared to both residents and academic experts. Scientists, on the other hand, stressed the need for stricter regulation of water demand. Findings point to the challenges of meshing different knowledge systems for collaborative research and policy making.     

Ecosystem services and urban heat riskscape moderation: water, green spaces, and social inequality in Phoenix, USA

Urban ecosystems are subjected to high temperatures--extreme heat events, chronically hot weather, or both-through interactions between local and global climate processes. Urban vegetation may provide a cooling ecosystem service, although many knowledge gaps exist in the biophysical and social dynamics of using this service to reduce climate extremes. To better understand patterns of urban vegetated cooling, the potential water requirements to supply these services, and differential access to these services between residential neighborhoods, we evaluated three decades (1970-2000) of land surface characteristics and residential segregation by income in the Phoenix, Arizona, USA metropolitan region. We developed an ecosystem service trade-offs approach to assess the urban heat riskscape, defined as the spatial variation in risk exposure and potential human vulnerability to extreme heat. In this region, vegetation provided nearly a 25 degrees C surface cooling compared to bare soil on low-humidity summer days; the magnitude of this service was strongly coupled to air temperature and vapor pressure deficits. To estimate the water loss associated with land-surface cooling, we applied a surface energy balance model. Our initial estimates suggest 2.7 mm/d of water may be used in supplying cooling ecosystem services in the Phoenix region on a summer day. The availability and corresponding resource use requirements of these ecosystem services had a strongly positive relationship with neighborhood income in the year 2000. However, economic stratification in access to services is a recent development: no vegetation-income relationship was observed in 1970, and a clear trend of increasing correlation was evident through 2000. To alleviate neighborhood inequality in risks from extreme heat through increased vegetation and evaporative cooling, large increases in regional water use would be required. Together, these results suggest the need for a systems evaluation of the benefits, costs, spatial structure, and temporal trajectory for the use of ecosystem services to moderate climate extremes. Increasing vegetation is one strategy for moderating regional climate changes in urban areas and simultaneously providing multiple ecosystem services. However, vegetation has economic, water, and social equity implications that vary dramatically across neighborhoods and need to be managed through informed environmental policies.     

Detecting Invasive Sericea Lespedeza (<Emphasis Type="Italic">Lespedeza cuneata</Emphasis>) in Mid-Missouri Pastureland Using Hyperspectral Imagery

Missouri ranks second in cow-calf numbers in the United States and its pastureland has experienced invasion of various plant species. As one of the invasive weeds, sericea lespedeza is becoming a serious threat to pasturelands in this state. The in-situ field survey in these vast pastures is time consuming and often impossible because of accessibility. Typical aerial survey is also difficult to detect sericea because the plant is of similar size and color as natural grass and, thus, cannot be effectively discriminated in broadband aerial color photos. This study used an airborne hyperspectral image to map sericea and its invasiveness in a public grass field in Mid-Missouri. The maximal 1st-order derivative in red-near infrared region (650–800nm) was derived to separate sericea from fescue, the dominant grass in pastures in Missouri. With a simple threshold approach, sericea of various sizes were identified in the study area. It was also found that the maximal 1st-order derivatives of sericea patches were log-linearly related to sericea “volume,” a quasi 3-dimensional biophysical variable as an approximate measure of sericea invasiveness. The squared correlation coefficient (r ²) of the regression was 0.65 and the estimation error of sericea “volume” estimation was 11% based on ground measurements at 27 sample sites. With this empirical regression model, the quantitative distribution of sericea volume was mapped, which could serve as a first step in alerting landowners and the general public about the seriousness of sericea invasion in Missouri pasturelands.     

Combined thermal and zero‐valent iron In Situ soil mixing remediation technology

Thermal remediation of contaminated soils and groundwater by injection of hot air and steam using large‐diameter auger in situ soil mixing effectively remediates volatile and semivolatile organic compounds. This technology removes large amounts of contamination during the early treatment stages, but extended treatment times are needed to achieve high removal percentages. Combining thermal treatment with another technology that can be injected and mixed into the soil, and that continues to operate after removal of the drilling equipment, improves removal efficiency, and reduces cost. Using field‐determined pseudo first‐order removal rates, the cost of the combined remediation of chlorinated volatile organic compounds (CVOCs) by thermal treatment followed by reductive dechlorination by iron powder has been estimated as 57 percent of the cost of thermal treatment alone. This analysis was applied to a case‐study remediation of 48,455 cubic yards, which confirmed the cost estimate of the combined approach and showed over 99.8 percent removal of trichloroethene and other chlorinated VOCs. © 2010 Wiley Periodicals, Inc.