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ROMSAIYUD Angsana SONGKASIRI Warinthom NOPHARATANA Annop CHAIPRASERT Pawinee 《环境科学学报(英文版)》2009,21(7)
The productivity and efficiency of cellulase are significant in cellulose hydrolysis. With the accumulation of volatile fatty acids (VFAs), the pH value in anaerobic digestion system is reduced. Therefore, this study will find out how the pH and the amount of acetate influence the enzymatic hydrolysis of cellulose. The effects of pH and acetate on cellulase produced from Bacillus coagulans were studied at various pH 5-8, and acetate concentrations (0-60 mmol/L). A batch kinetic model for enzymatic cellulose hydrolysis was constructed from experimental data and performed. The base hypothesis was as follows: the rates of enzymatic cellulose hydrolysis rely on pH and acetate concentration. The results showed that the suitable pH range for cellulase production and cellulose hydrolysis (represents efficiency of cellulase) was 2.6-7.5, and 5.3-8.3, respectively. Moreover, acetate in the culture medium had an effect on cellulase production (K1= 49.50 mmol/L, n=1.7) less than cellulose hydrolysis (K1=37.85 mmol/L, n=2.0). The results indicated that both the pH of suspension and acidogenic products influence the enzymatic hydrolysis of cellulose in an anaerobic environment. To enhance the cellulose hydrolysis rate, the accumulated acetate concentration should be lower than 25 mmol/L, and pH should be maintained at 7. 相似文献
2.
Decolorization of molasses melanoidins and palm oil mill effluent phenolic compounds by fermentative lactic acid bacteria 总被引:1,自引:0,他引:1
Lactobacillus plantarum SF5.6 is one of the lactic acid bacteria (LAB) that has the highest ability of molasses melanoidin (MM) decolorization among the 2114 strains of LAB. The strains were isolated from spoilage, pickle fruit and vegetable, soil and sludge from the wastewater treatment system by using technical step of enrichment, primary screening and secondary screening. This LAB strain SF5.6 was identified by 16S rDNA analysis and carbohydrate fermentation (API 50 CH). The top five LAB strains having high MM decolorization ( 55%), namely TBSF5.8-1, TBSF2.1-1, TBSF2.1, FF4A and SF5.6 were selected to determine the optimal condition. It was found that the temperature at 30°C under facultative conditions in GPY-MM medium (0.5% glucose, 0.1% peptone, 0.1% yeast extract, 0.1% sodium acetate, 0.05% MgSO4 and 0.005% MnCl2 in MM solution at pH 6) giving a high microbial growth and MM decolorization for all five strains. It was noticed that the decolorization of MM by LAB strains might be cell growth associated. L. plantarum SF5.6 grew rapidly within one day while the other strains took 2–3 days. This L. plantarum SF5.6 could rapidly decolorize MM to 60.91% without any lag phase, and it also had the ability to remove 34.00% phenolic compounds and 15.88% color from treated palm oil mill effluent. 相似文献
3.
Lactobacillus plantarum SF5.6 is one of the lactic acid bacteria (LAB) that has the highest ability of molasses melanoidin (MM)
decolorization among the 2114 strains of LAB. The strains were isolated from spoilage, pickle fruit and vegetable, soil and sludge from
the wastewater treatment system by using technical step of enrichment, primary screening and secondary screening. This LAB strain
SF5.6 was identified by 16S rDNA analysis and carbohydrate fermentation (API 50 CH). The top five LAB strains having high MM
decolorization (> 55%), namely TBSF5.8-1, TBSF2.1-1, TBSF2.1, FF4A and SF5.6 were selected to determine the optimal condition.
It was found that the temperature at 30°C under facultative conditions in GPY-MM medium (0.5% glucose, 0.1% peptone, 0.1% yeast
extract, 0.1% sodium acetate, 0.05% MgSO4 and 0.005% MnCl2 in MM solution at pH 6) giving a high microbial growth and MM
decolorization for all five strains. It was noticed that the decolorization of MM by LAB strains might be cell growth associated. L.
plantarum SF5.6 grew rapidly within one day while the other strains took 2–3 days. This L. plantarum SF5.6 could rapidly decolorize
MM to 60.91% without any lag phase, and it also had the ability to remove 34.00% phenolic compounds and 15.88% color from treated
palm oil mill e uent. 相似文献
4.
Sanphoti N Towprayoon S Chaiprasert P Nopharatana A 《Journal of environmental management》2006,81(1):27-35
In order to increase methane production efficiency, leachate recirculation is applied in landfills to increase moisture content and circulate organic matter back into the landfill cell. In the case of tropical landfills, where high temperature and evaporation occurs, leachate recirculation may not be enough to maintain the moisture content, therefore supplemental water addition into the cell is an option that could help stabilize moisture levels as well as stimulate biological activity. The objectives of this study were to determine the effects of leachate recirculation and supplemental water addition on municipal solid waste decomposition and methane production in three anaerobic digestion reactors. Anaerobic digestion with leachate recirculation and supplemental water addition showed the highest performance in terms of cumulative methane production and the stabilization period time required. It produced an accumulated methane production of 54.87 l/kg dry weight of MSW at an average rate of 0.58 l/kg dry weight/d and reached the stabilization phase on day 180. The leachate recirculation reactor provided 17.04 l/kg dry weight at a rate of 0.14l/kg dry weight/d and reached the stabilization phase on day 290. The control reactor provided 9.02 l/kg dry weight at a rate of 0.10 l/kg dry weight/d, and reached the stabilization phase on day 270. Increasing the organic loading rate (OLR) after the waste had reached the stabilization phase made it possible to increase the methane content of the gas, the methane production rate, and the COD removal. Comparison of the reactors' efficiencies at maximum OLR (5 kgCOD/m(3)/d) in terms of the methane production rate showed that the reactor using leachate recirculation with supplemental water addition still gave the highest performance (1.56 l/kg dry weight/d), whereas the leachate recirculation reactor and the control reactor provided 0.69 l/kg dry weight/d and 0.43 l/kg dry weight/d, respectively. However, when considering methane composition (average 63.09%) and COD removal (average 90.60%), slight differences were found among these three reactors. 相似文献
5.
ROMSAIYUD Angsan SONGKASIRI Warinthorn NOPHARATANA Annop CHAIPRASERT Pawinee 《环境科学学报(英文版)》2009,21(7):965-970
The productivity and e ciency of cellulase are significant in cellulose hydrolysis. With the accumulation of volatile fatty acids
(VFAs), the pH value in anaerobic digestion system is reduced. Therefore, this study will find out how the pH and the amount of acetate
influence the enzymatic hydrolysis of cellulose. The e ects of pH and acetate on cellulase produced from Bacillus coagulans were
studied at various pH 5–8, and acetate concentrations (0–60 mmol/L). A batch kinetic model for enzymatic cellulose hydrolysis was
constructed from experimental data and performed. The base hypothesis was as follows: the rates of enzymatic cellulose hydrolysis
rely on pH and acetate concentration. The results showed that the suitable pH range for cellulase production and cellulose hydrolysis
(represents e ciency of cellulase) was 2.6–7.5, and 5.3–8.3, respectively. Moreover, acetate in the culture medium had an e ect on
cellulase production (KI = 49.50 mmol/L, n = 1.7) less than cellulose hydrolysis (KI = 37.85 mmol/L, n = 2.0). The results indicated
that both the pH of suspension and acidogenic products influence the enzymatic hydrolysis of cellulose in an anaerobic environment.
To enhance the cellulose hydrolysis rate, the accumulated acetate concentration should be lower than 25 mmol/L, and pH should be
maintained at 7. 相似文献
6.
Based on the developed neural-fuzzy control system for anaerobic hybrid reactor (AHR) in wastewater treatment and biogas
production, the neural network with backpropagation algorithm for prediction of the variables pH, alkalinity (Alk) and total volatile
acids (TVA) at present day time t was used as input data for the fuzzy logic to calculate the influent feed flow rate that was applied
to control and monitor the process response at di erent operations in the initial, overload influent feeding and the recovery phases. In
all three phases, this neural-fuzzy control system showed great potential to control AHR in high stability and performance and quick
response. Although in the overloading operation phase II with two fold calculating influent flow rate together with a two fold organic
loading rate (OLR), this control system had rapid response and was sensitive to the intended overload. When the influent feeding rate
was followed by the calculation of control system in the initial operation phase I and the recovery operation phase III, it was found that
the neural-fuzzy control system application was capable of controlling the AHR in a good manner with the pH close to 7, TVA/Alk <
0.4 and COD removal > 80% with biogas and methane yields at 0.45 and 0.30 m3/kg COD removed. 相似文献
7.
Laowansiri S Vinitnantharat S Chaiprasert P Ha SR 《Journal of environmental biology / Academy of Environmental Biology, India》2008,29(3):309-314
This study aims to investigate the anaerobic degradation kinetics of reactive dye, C.I. Reactive Red 141 (Evercion Red H-E7B) by partially granulated anaerobic mixed culture using three carbon sources, namely modified starch (MS), polyvinyl alcohol (PVA) and acrylic size (AS) during batch incubation. There is a first-order kinetics reaction in the decolorization processes using MS and PVA as carbon sources, while a zero-order kinetics relationship describes the decolorization process for the AS carbon source. The k values and color removal rate of decolorization with MS carbon source was higher than those of PVA and AS carbon sources. This is because the MS carbon source was well degraded in comparison to AS and PVA, respectively This study also found dye reduction could be enhanced through the addition of MS as a carbon source. The decolorization rates increased with decrease in dye concentrations of RR 141. In contrast, the decolorization rates increased with increase in COD concentration. 相似文献
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