Evaluation of pollution by heavy metals of an abandoned Pb-Zn mine in northern Tunisia using sequential fractionation and geostatistical mapping

This study investigates the contents of lead, zinc, and cadmium in 109 near-surface soil samples collected around the abandoned mine of Fedj Lahdoum, northern Tunisia, to assess the risk of pollution they generate. The study involved some analytical procedures such as pH measurements, X-ray diffraction techniques, sequential fractionation, and geostatistical mapping using the ordinary Kriging techniques. The sequential fractionation revealed that the bioavailability of Pb, Zn, and Cd follows the orders F5?>?F3 » F4?>?F2 » F1, F5?>?F3 » F4 » F2?>?F1 and F5?>?F2 » F4?>?F1, respectively; their associations with organic matter and residual sulfides (F4) are relatively low. However, their high cumulated contents are dominantly associated with the residual (F5) and reducible (F3) fractions. The geostatistical mapping was endeavored to predict the spatial distribution of the studied heavy metals at unsampled sites and to produce a cumulated risk map of soil pollution. The latter is discussed with emphasis of the main factors responsible for the scattering of the pollution as much as the landscape conditions, the chemical composition of the mine tailings, the surface drainage of meteoric water and the wind. This study provides insight into the delineation of the spatial spreading of Pb, Zn, and Cd around the abandoned mine Fedj Lahdoum and their surrounding urban areas. It reveals that the mine infrastructure areas encompassing both extraction and processing and tailing deposition areas are the main sources of contamination. And the landscape conditions together with the surface drainage of meteoric water and the wind are the main factors responsible for the scattering of the pollution.

     

Risk assessment of heavy metal toxicity of soil irrigated with treated wastewater using heat shock proteins stress responses: case of El Hajeb,Sfax, Tunisia

Heavy metal contamination of soil resulting from treated wastewater irrigation can cause serious concerns resulting from consuming contaminated crops. Therefore, it is crucial to assess hazard related to wastewater reuse. In the present investigation, we suggest the use of biomarker approach as a new tool for risk assessment of wastewater reuse in irrigation as an improvement to the conventional detection of physicochemical accumulation in irrigated sites. A field study was conducted at two major sites irrigated with treated wastewater and comparisons were made with a control site. Different soil depths were considered to investigate the extent of heavy metal leaching, the estrogenic activity, and the biomarker response. Results have shown that a longer irrigation period (20 years) caused a slight decrease in soil metal levels when compared to the soil irrigated for 12 years. The highest levels of Cr, Co, Ni, Pb, and Zn were detected at 20 and 40 cm horizons in plots irrigated with wastewater for 12 years. The latter finding could be attributed to chemical leaching to deeper plots for longer irrigation period. Furthermore, the treated wastewater sample showed a high estrogenic activity while none of the soil samples could induce any estrogenic activity. Regarding the stress response, it was observed that the highest stress shown by the HSP47 promoter transfected cells was induced by a longer irrigation period. Finally, the treated wastewater and the irrigated soils exhibited an overexpression of HSP60 in comparison with reference soil following 1 h exposure. In conclusion, in vitro techniques can be efficiently used to assess potential hazard related to wastewater reuse.     

Heat shock protein 47 stress responses in Chinese hamster ovary cells exposed to raw and reclaimed wastewater

As wastewater reclamation and reuse becomes more widespread, risks of exposure to treated wastewater increase. Moreover, an unlimited number of pollutants can be identified in wastewater. Therefore, comprehensive toxicity assessment of treated wastewater is imperative. The objective of this study was to perform a comprehensive toxicity assessment of wastewater treatment systems using stress response bioassays. This powerful tool can comprehensively assess the toxicity of contaminants. In this study, samples from conventional activated sludge treatment, membrane bioreactors (MBRs) with different pore sizes and sludge retention times (SRTs), rapid sand filtration, coagulation, nano-filtration (NF) and reverse osmosis (RO) were investigated. The results of stress response bioassays confirmed that the secondary effluent showed higher stress response than influent indicating that biological treatment generates toxic compounds. The results obtained from molecular weight fractionation of water samples demonstrated that organic matter with a higher molecular weight fraction (>0.1 μm) causes toxicity in secondary effluent. Furthermore, supernatant from MBR reactors showed toxicity regardless of SRT. On the other hand, stress response was not detected in MBR permeates except for an MBR equipped with a larger pore size membrane (0.4 μm) and with a short SRT (12 days). While rapid sand filtration could not remove the toxic compounds found in secondary effluent, coagulation tests, operated at an appropriate pH, were effective for reducing stress response in the secondary effluent. Experimental findings also showed that stress response was not detected in cases of NF and RO permeate subsequent to MBR treatment.     

Deducing an upper bound to the horizontal eddy diffusivity using a stochastic Lagrangian model

We present a method for estimating the upper bound of the horizontal eddy diffusivity using a non-stationary Lagrangian stochastic model. First, we identify a mixing barrier using a priori evidence (e.g., aerial photographs or satellite imagery) and using a Lagrangian diagnostic calculated from observed or modeled spatially non-trivial, time-dependent velocities [for instance, the relative dispersion (RD) or finite time Lyapunov exponent (FDLE)]. Second, we add a stochastic component to the observed (or modeled) velocity field. The stochastic component represents sub-grid stochastic diffusion and its mean magnitude is related to the eddy diffusivity. The RD of Lagrangian trajectories is computed for increasing values of the eddy diffusivity until the mixing barrier is no longer present. The value at which the mixing barrier disappears provides a dynamical estimate of the upper bound of the eddy diffusivity. The erosion of the mixing barrier is visually observed in numerical simulations, and is quantified by computing the kurtosis of the RD at each value of the eddy diffusivity. We demonstrate our method using the double gyre circulation model and apply it to high frequency (HF) radar observations of surface currents in the Gulf of Eilat.     

Induction de l'èmission des gamètes et premiers stades du développement larvaire chez l'astérie fissipare Sclerasterias richardi

Sclerasterias richardi, a seastar living on the border (120 to 200 m) of the Mediterranean Continental Shelf, displays fissiparity concomitant with sexual reproduction. In order to describe the as yet unknown larva, artificial fecundation was induced in specimens collected in October, 1975. To obtain gametes, the seastars were treated with a 3x10^-6 M 1-methyladenine solution injected into the coelomic cavity. Histological study of the gonads revealed that the low proportion of the treated sample responding positively was due to the experiments being conducted at the end of the natural spawning period. The behaviour of males during sperm emission is described and discussed. Our observations suggest that S. richardi would exhibit epidemic sexuality under natural conditions. The development of fertilized ova, observed over a period of 42 days, produces planktotrophic larvae, the first photographs of which are presented.     

Incentive mechanisms to enforce sustainable forest exploitation

Using a two-player differential game approach, this paper deals with the issue of tropical deforestation. The assumption is that developing forestry countries (called the South) do not, or cannot, necessarily have the same utility for forest conservation than the developed countries (called the North). Given the global nature of the problem, we assume that it may be wise that the North offers financial support to the South to help in achieving a sustainable forest policy. We derive sustainable and short-run deforestation policies and provide a transfer mechanism which insures that the South will indeed implement the sustainable one.