Prenatal diagnosis using deletion studies in Duchenne muscular dystrophy

Accurate carrier testing and prenatal diagnosis in Duchenne muscular dystrophy (DMD) families is facilitated when an Xp21 deletion is found to be segregating within a family. We discuss the results of the DNA testing in two families, one in which DNA from affected males was available for study and the other in which no DNA from an affected male was available. Factors complicating the counselling of DMD deletion families are outlined.     

Sludge-grown algae for culturing aquatic organisms: Part II. Sludge-grown algae as feeds for aquatic organisms

This project investigated the feasibility of using sewage sludge to culture microalgae (Chlorella-HKBU) and their subsequent usage as feeds for rearing different organisms. Part II of the project evaluated the results of applying the sludge-grown algae to feedOreochromis mossambicus (fish),Macrobrachium hainenese (shrimp), andMoina macrocopa (cladocera). In general, the yields of the cultivated organisms were unsatisfactory when they were fed the sludge-grown algae directly. The body weights ofO. mossambicus andM. macrocopa dropped 21% and 37%, respectively, although there was a slight increase (4.4%) inM. hainenese. However, when feeding the algal-fed cladocerans to fish and shrimp, the body weights of the fish and shrimp were increased 7% and 11% accordingly. Protein contents of the cultivated organisms were comparable to the control diet, although they contained a rather high amount of heavy metals. When comparing absolute heavy metal contents in the cultivated organisms, the following order was observed: alga > cladocera > shrimp, fish > sludge extracts. Bioelimination of heavy metals may account for the decreasing heavy metal concentrations in higher trophic organisms.     

A systemic health risk assessment for the chromium cycle in Taiwan

Health risk assessment (HRA) has been recognized as a useful tool for identifying health risks of human activities. In particular, this method has been well applied to spatially defined units, such as a production plant, a treatment facility, and a contaminated site. However, the management strategies based on the risk information will be more efficient if the comprehensive picture of total risks from all kinds of sources is depicted. In principle, the total risks can be obtained when all risk sources are assessed individually. Apparently, this approach demands huge amount of efforts. This study develops a methodology that combines substance flow and risk estimation to facilitate examination of risk in a systemic way and provide comprehensive understanding of risk generation and distribution corresponding to flows of substances in the anthroposphere and the environment. Substance flow analysis (SFA) and HRA method is integrated to produce a systemic risk assessment method, from which substance management schemes can be derived. In this study, the chromium cycle in Taiwan is used as an example to demonstrate the method, by which the associated substance flow in the economy and the risk caused by the substance in the environmental system is determined. The concentrations of pollutants in the environmental media, the resultant risks and hazard quotients are calculated with the widely-used CalTOX multimedia model.     

Comprehensive temperature monitoring in an in-vessel forced-aeration static-bed composting process

Comprehensive temperature monitoring was done in an in-vessel forced-aeration static-bed composting process using sewage sludge. The three layers that divided the compost pile horizontally showed different temperature distributions. The temperature of the top layer appeared not to be influenced by the ambient temperature. The temperature of the center area of the top layer was taken to be the representative temperature for evaluating composting start-up performance. The temperature of the bottom layer was strongly influenced by the ambient temperature, and the temperature of the center area of the bottom layer was taken to be the representative temperature for pathogen control as it was the minimum temperature in the reactor. Composting start-up performance was influenced by several factors when the ambient temperature was either below or above 20°C. When the ambient temperature was below 20°C, the time taken to reach 65°C (T ₆₅) was influenced by the temperature of inflowing air, but when the temperature was higher than 20°C, it was influenced by the ratio of sewage sludge to seed compost (F/S). T ₆₅ was least when F/S was 1–2. Received: January 9, 2001 / Accepted: October 10, 2001     

An algorithm for assessing the risk of traffic accident

INTRODUCTION: This study is aimed at developing an algorithm to estimate the number of traffic accidents and assess the risk of traffic accidents in a study area. METHOD: The algorithm involves a combination of mapping technique (Geographical Information System (GIS) techniques) and statistical methods (cluster analysis and regression analysis). Geographical Information System is used to locate accidents on a digital map and realize their distribution. Cluster analysis is used to group the homogeneous data together. Regression analysis is performed to realize the relation between the number of accident events and the potential causal factors. Negative binomial regression model is found to be an appropriate mathematical form to mimic this relation. Accident risk of the area, derived from historical accident records and causal factors, is also determined in the algorithm. The risk is computed using the Empirical Bayes (EB) approach. A case study of Hong Kong is presented to illustrate the effectiveness of the proposed algorithm. RESULTS: The results show that the algorithm improves accident risk estimation when comparing to the estimated risk based on only the historical accident records. The algorithm is found to be more efficient, especially in the case of fatality and pedestrian-related accident analysis. IMPACT ON INDUSTRY: The output of the proposed algorithm can help authorities effectively identify areas with high accident risk. In addition, it can serve as a reference for town planners considering road safety.     

Removal of organic toxic chemicals using the spent mushroom compost of Ganoderma lucidum

The removal of the organic toxic chemicals di-n-butyl phthalate (DBP), di-2-ethyl hexyl phthalate (DEHP), nonylphenol (NP), and bisphenol-A (BPA) by laccase obtained from the spent mushroom compost (SMC) of the white rot fungi, Ganoderma lucidum, was investigated. The optimal conditions for the extraction of laccase from SMC required using sodium acetate buffer (pH 5.0, solid : solution ratio 1 : 5), and extraction over 3 h at 4 °C. The removal of NP was enhanced by adding CuSO(4) (1 mM), MnSO(4) (0.5 mM), tartaric acid (20 mM), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS; 1 mM), and 1-hydroxybenzotriazole (HBT; 20 mg L(-1)), with ABTS yielding a higher NP removal efficiency than the other additives. At a concentration of 2 mg L(-1), DBP, DEHP, NP, and BPA were almost entirely removed by laccase after incubation for 1 day. The removal efficiencies, in descending order of magnitude, were DBP > BPA > NP > DEHP. We believe that these findings could provide useful information for improving the efficiency of the removal of organic toxic chemicals in the environment.     

Sources and seasonal variation of PAHs in the sediments of drinking water reservoirs in Hong Kong and the Dongjiang River (China)

The main objective of this study was to investigate occurrence of polycyclic aromatic hydrocarbons (PAHs) in the sources of the drinking water supply of Hong Kong. The main emphasis was on the Dongjiang River in mainland China which is the major source, supplying 80% of the total consumption in Hong Kong (the remaining 20% is obtained from rain water). Sediments were collected from four sites along the Dongjiang River and four reservoirs in Hong Kong during both the dry and wet weather seasons. The concentrations of total PAHs in the sediments ranged between 36 and 539 microg/kg dry wt. The lower levels were detected at the upstream site on the Dongjiang River and at the reservoirs in Hong Kong (44-85 microg/kg dry wt), while the mid- and downstream sites on the Dongjiang River were more polluted (588-658 microg/kg dry wt). Examination of the PAH profiles revealed that the mid- and downstream sections of the Dongjiang River contained high percentages of 4,5,6-ring PAHs, similar to the amounts of atmospheric particulate matter and road dust collected during the dry weather season from the Pearl River Delta region as reported in the literature. Seasonal changes were revealed in the reservoirs of Hong Kong, with higher PAH levels in the wet weather season than in the dry weather season. For those reservoirs in Hong Kong that store water from the Dongjiang River, a distinct seasonal pattern was also observed, namely, that under dry weather season conditions the PAHs found in the sediments were primarily from petrogenic source, while under wet weather season conditions they were from pyrolytic sources. No such pattern was detected in the reservoirs which stored only rain water.