Bacterial degradation of monocrotophos and phyto- and cyto-toxicological evaluation of metabolites

The bacterium Serratia marsescens strain JAS16 was isolated from agricultural soil which had prior exposure to monocrotophos for three years. The strain JAS16 tolerated up to 1200 mg L^–1 monocrotophos and degraded the insecticide (1000 mg L^–1) at a degradation rate constant of 136 d^?1 (DT₅₀ = 3.7 d). In soil, the degradation rate constant was 105 d^?1 (DT₅₀ = 4.8 d). A schematic pathway is being proposed from the degraded products derived from gas chromatography--mass spectrometry (GC-MS). The phytotoxicity of degradation products to Vigna radiata, Vigna unguiculata, and Macrotyloma uniflorum and the genotoxicity to Allium cepa roots were found to be low. A cost-effective powder-based formulation was achieved with the isolate. The isolate remained viable during the storage and also multiplied with a higher colony forming units (CFU) load g^–1 for over a period of seven weeks of storage.     

Shoreline change and potential sea level rise impacts in a climate hazardous location in southeast coast of India

Climate change impact on the environment makes the coastal areas vulnerable and demands the evaluation of such susceptibility. Historical changes in the shoreline positions and inundation based on projected sea-level scenarios of 0.5 and 1 m were assessed for Nagapattinam District, a low-lying coastal area in the southeast coast of India, using high-resolution Shuttle Radar Topography Mission data; multi-dated Landsat satellite images of 1978, 1991, 2003, and 2015; and census data of 2011. Image processing, geographical information system, and digital shoreline analysis system methods were used in the study. The shoreline variation indicated that erosion rate varied at different time scales. The end point rate indicated the highest mean erosion of ??3.12 m/year, occurred in 73% of coast between 1978 and 1991. Weighted linear regression analysis revealed that the coast length of 83% was under erosion at a mean rate of ??2.11 m/year from 1978 to 2015. Sea level rise (SLR) impact indicated that the coastal area of about 14,122 ha from 225 villages and 31,318 ha from 272 villages would be permanently inundated for the SLR of 0.5 and 1 m, respectively, which includes agriculture, mangroves, wetlands, aquaculture, and forest lands. The loss of coastal wetlands and its associated productivity will severely threaten more than half the coastal population. Adaptation measures in people participatory mode, integrated into coastal zone management with a focus on sub-regional coastal activities, are needed to respond to the consequences of climate change.     

Distribution of persistent organochlorine chemical residues in blood plasma of three species of vultures from India

The presence of persistent organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) were determined in blood plasma of white-backed vulture Gyps bengalensis, Egyptian vulture Neophron percnopterus, and griffon vulture Gyps fulvus collected from Ahmedabad, India. All the samples had varying levels of organochlorine pesticides and PCBs. Statistically significant (P?<?0.05) differences among species were detected for beta-hexachlorocyclohexane (??-HCH), ??HCH, and dichloro-diphenyl-trichloroethane (DDT). The mean concentration of ??HCH, ??DDT, and ??PCBs among plasma ranged from 43.7 to 136, 8.8 to 64.8, and 226 to 585 ng/ml, respectively. Among the various OCPs analyzed, 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p ^??-DDE) was detected most frequently. The concentrations of cyclodiene insecticides detected were lower than the other organochlorine residues. The levels of pesticides measured in plasma samples of three species of vulture were comparable to the results documented for a number of avian species and were lower than those reported to have deleterious effects on survival or reproduction of birds. Although no threat is posed by any of the organochlorine pesticides detected, continuous monitoring of breeding colonies is recommended. This study is also the first account of a comprehensive analysis of toxicants present in blood plasma of vulture species in India. The values reported in this study can serve as guidelines for future research in general as well as control values during the analysis of samples obtained from birds in the event of suspected organochlorine poisoning.     

A design for the additive manufacture of functionally graded porous structures with tailored mechanical properties for biomedical applications

CAD/CAM-based layered manufacturing and additive manufacturing techniques of metals have found applications in near-net-shape fabrication of complex shaped parts with tailored mechanical properties for several applications. Especially with the onset of newer processes such as electron beam melting (EBM) and direct metal laser sintering (DMLS), revolutionary advances may be achieved in material substitution in the medical implant industry. These processes must be suitably developed and tested for the production of medical grade substitutions. In this article, we discuss a design process for creating periodic cellular structures specifically targeted for biomedical applications. Electron beam melting is used to fabricate the parts. Evaluation of the mechanical properties is performed and compared with design parameters. Compression tests of the samples show effective stiffness values ranging from 0.57 (±0.05) to 2.92 (±0.17) GPa and compressive strength values of 7.28 (±0.93) to 163.02 (±11.98) MPa. Substituting these values for simulation of biomechanical performance of patient-specific implants illustrates the compatibility and matched functional performance characteristics of highly porous parts at a safety factor of 5 and an effective reduction in weight. These developments are unique for the construction of maxillofacial and craniofacial implants. The novel design strategy also lends itself very well to metal additive manufacturing technologies. Implants designed and fabricated with this design strategy and manufacturing process would have mechanical properties equivalent to the part they replace and restore better function and esthetics as against the currently used methods of reconstruction. Suitable examples of a titanium porous cranioplasty plate and a sandwich structure are illustrated.     

Nanostructured semiconducting materials for efficient hydrogen generation

Massive production of hydrogen by water decomposition triggered by a solar light active photocatalyst is a major objective in chemistry and a promising avenue to overcome the global energy crisis. The development of efficient, stable, economically viable and eco-friendly photocatalysts for hydrogen production is a challenging task. This article reviews the use of nanocomposite in three combinations: metal oxide–metal oxide semiconductor, metal–metal oxide semiconductor and metal chalcogenide–metal oxide core–shell nanostructures. These core–shell structures occur in two forms: a simple form where the photocatalyst is either in the core or the shell or in a more complex system where the core–shell structure comprises a co-catalyst deposited on a semiconducting material. We discuss the design, synthesis and development of semiconductor-based nanocomposite photocatalysts for hydrogen production. The major points are the role of catalytic active sites, the chemical nature of sacrificial agents, the effect of light sources, the variable light intensity and the energy efficiency calculation. For TiO₂-based nanocomposites, the metal oxide or metal co-catalyst loading of 1.0–3.0 wt% was optimal. TiO₂ nanotube–CuO hybrid nanocomposites produce 1,14,000 µmol h^?1 \({\text{g}}^{ - 1}_{\text{cat}}\), whereas TiO₂/Au nanocomposites display 1,60,000 µmol h^?1 \({\text{g}}^{ - 1}_{\text{cat}}\). For core–shell catalysts, a shell thickness of 2–20 nm was found for the best activity, and its performance is as follows: (a) CdS–NiO system produces around 19,949 µmol h^?1 \({\text{g}}^{ - 1}_{\text{cat}}\) and (b) CuO–Cr₂O₃ as co-catalyst immobilized on TiO₂ system produces around 82,390 µmol h^?1 \({\text{g}}^{ - 1}_{\text{cat}}\).