Leaching of cyanogenic glucosides and cyanide from white clover green manure

Use of crops for green manure as a substitute for chemical fertilizers and pesticides is an important approach towards more sustainable agricultural practices. Green manure from white clover is rich in nitrogen but white clover also produces the cyanogenic glucosides (CGs) linamarin and lotaustralin; CGs release toxic hydrogen cyanide (HCN) upon hydrolysis which may be utilized for pest control. We demonstrate that applying CGs in the form of a liquid extract of white clover to large columns of intact agricultural soils can result in leaching of toxic cyanide species to a depth of at least 1m. Although degradation of the CGs during leaching proceeded with half lives in the interval 1.5-35 h depending on soil characteristics, a fraction of the applied CGs (0.9-3.2%) was recovered in the leachate as either CGs or toxic cyanide species. Detoxification of the HCN formed was rapid in soil and leachate from both sandy and loamy soil. However, 30% of the leachate samples exceeded the EU threshold value of 50 micrgl(-1) total cyanide for drinking water and 85% exceeded the US threshold of 5 micrgl(-1) for cyanide chronic ecotoxicity in fresh water. This study demonstrates that even easily degradable natural products present in crop plants as defense compounds pose a threat to the quality of groundwater and surface waters. This aspect needs consideration in assessment of the risk associated with use of crops as green manure to replace chemical fertilizers and pesticides as well as in genetic engineering approaches to design crops with improved pest resistance.     

The interaction between physical and social-psychological factors in indoor environmental health

Indoor environmental health is now recognized as an important factor in preventing respiratory health problems in the United States. It is also a concern in Canada due to the amount of time that Canadians spend indoors because of cold weather and the potential for increased time indoors during the summer if theclimate warms. The negative health effects are often labeled assick building syndrome, but diagnosing a building or itsoccupants as sick is complicated by the variety of symptoms, thepresence of chronic versus acute symptoms and social andpsychological (socio-psychological) factors that may reduce theeffectiveness of an engineering solution. As a case study, thecontribution of various factors to indoor environmental health,in three buildings at the University of Toronto, was examinedusing five different methods. The results indicate that theinhabitants of the buildings consider features other than airquality in considering building health such as design,maintenance, funding cuts and socio-psychological factors.     

Characterizing spatial structure of sediment E. coli populations to inform sampling design

Escherichia coli can persist in streambed sediments and influence water quality monitoring programs through their resuspension into overlying waters. This study examined the spatial patterns in E. coli concentration and population structure within streambed morphological features during baseflow and following stormflow to inform sampling strategies for representative characterization of E. coli populations within a stream reach. E. coli concentrations in bed sediments were significantly different (p?=?0.002) among monitoring sites during baseflow, and significant interactive effects (p?=?0.002) occurred among monitoring sites and morphological features following stormflow. Least absolute shrinkage and selection operator (LASSO) regression revealed that water velocity and effective particle size (D ₁₀) explained E. coli concentration during baseflow, whereas sediment organic carbon, water velocity and median particle diameter (D ₅₀) were important explanatory variables following stormflow. Principle Coordinate Analysis illustrated the site-scale differences in sediment E. coli populations between disconnected stream segments. Also, E. coli populations were similar among depositional features within a reach, but differed in relation to high velocity features (e.g., riffles). Canonical correspondence analysis resolved that E. coli population structure was primarily explained by spatial (26.9–31.7 %) over environmental variables (9.2–13.1 %). Spatial autocorrelation existed among monitoring sites and morphological features for both sampling events, and gradients in mean particle diameter and water velocity influenced E. coli population structure for the baseflow and stormflow sampling events, respectively. Representative characterization of streambed E. coli requires sampling of depositional and high velocity environments to accommodate strain selectivity among these features owing to sediment and water velocity heterogeneity.     

Connecting the dots: mapping habitat connectivity for tigers in central India

Large connected landscapes are paramount to maintain top predator populations. Across their range, tiger (Panthera tigris) populations occur in small fragmented patches of habitat, often isolated by large distances in human-dominated landscapes. We assessed connectivity between 16 protected areas (PAs) in central India, a global priority landscape for tiger conservation, using data on land use and land cover, human population density, and transportation infrastructure. We identified and prioritized movement routes using a combination of least-cost corridor modeling and circuit theory. Our analyses suggest that there are several opportunities to maintain connectivity in this landscape. We mapped a total of thirty-five linkages in the region and calculated metrics to estimate their quality and importance. The highest quality linkages as measured by the ratio of cost-weighted distance to Euclidean distance are Kanha–Phen/Bandhavgarh–SanjayGhasidas/Melghat–Satpura, and cost-weighted distance to least-cost path length are Nawegaon–Tadoba/Achanakmar–SanjayGhasidas/Kanha–Phen. We used current flow centrality to evaluate the contribution of each PA and linkage toward facilitating animal movement. Values are highest for Kanha and Pench tiger reserves, and the linkages between Kanha–Phen, Kanha–Pench, and Pench–Satpura, suggesting that these PAs and linkages play a critical role in maintaining connectivity in central India. In addition, smaller areas such as Bor, Nawegaon, and Phen have high centrality scores relative to their areas and thus may act as important stepping stones. We mapped pinch points, which are sections of the linkages where tiger movement is restricted due to unfavorable habitat, transportation networks, human habitation, or a combination of factors. Currently, very limited data exist on tiger movement outside of PAs to validate model results. Regional-scale connectivity mapping efforts can assist managers and policy makers to develop strategic plans for balancing wildlife conservation and other land uses in the landscape.     

Circumpolar status of Arctic ptarmigan: Population dynamics and trends

Rock ptarmigan (Lagopus muta) and willow ptarmigan (L. lagopus) are Arctic birds with a circumpolar distribution but there is limited knowledge about their status and trends across their circumpolar distribution. Here, we compiled information from 90 ptarmigan study sites from 7 Arctic countries, where almost half of the sites are still monitored. Rock ptarmigan showed an overall negative trend on Iceland and Greenland, while Svalbard and Newfoundland had positive trends, and no significant trends in Alaska. For willow ptarmigan, there was a negative trend in mid-Sweden and eastern Russia, while northern Fennoscandia, North America and Newfoundland had no significant trends. Both species displayed some periods with population cycles (short 3–6 years and long 9–12 years), but cyclicity changed through time for both species. We propose that simple, cost-efficient systematic surveys that capture the main feature of ptarmigan population dynamics can form the basis for citizen science efforts in order to fill knowledge gaps for the many regions that lack systematic ptarmigan monitoring programs.

     

Phytoremediation of a Petroleum‐Hydrocarbon Contaminated Shallow Aquifer in Elizabeth City,North Carolina,USA

A former bulk fuel terminal in North Carolina is a groundwater phytoremediation demonstration site where 3,250 hybrid poplars, willows, and pine trees were planted from 2006 to 2008 over approximately 579,000 L of residual gasoline, diesel, and jet fuel. Since 2011, the groundwater altitude is lower in the area with trees than outside the planted area. Soil‐gas analyses showed a 95 percent mass loss for total petroleum hydrocarbons (TPH) and a 99 percent mass loss for benzene, toluene, ethylbenzene, and xylenes (BTEX). BTEX and methyl tert‐butyl ether concentrations have decreased in groundwater. Interpolations of free‐phase, fuel product gauging data show reduced thicknesses across the site and pooling of fuel product where poplar biomass is greatest. Isolated clusters of tree mortalities have persisted in areas with high TPH and BTEX mass. Toxicity assays showed impaired water use for willows and poplars exposed to the site's fuel product, but Populus survival was higher than the willows or pines on‐site, even in a noncontaminated control area. All four Populus clones survived well at the site. © 2014 Wiley Periodicals, Inc.*     

Black Carbon Aerosol at McMurdo Station,Antarctica

ABSTRACT

Aerosol light absorption as black carbon (BC) was measured from November 19, 1995, to February 6, 1996, at a location 0.65 km downwind of the center of McMurdo Station on the Antarctic coast. The results show a bimo-dal frequency distribution of BC concentrations. Approximately 65% of the measurements were found in a mode at a low range of concentrations centered at ~20 ng/m³. These concentrations are higher than those found at other remote Antarctic locations and probably represent contamination from the station. The remaining measurements were in a high-concentration mode (BC ~300 ng/m³), indicating direct impact of local emissions from combustion activities at the station. High values of BC were associated with winds from the direction of the station, and the BC flux showed a clear directionality. Maximum BC concentrations occurred between 7:00 and 11:00 a.m. The "polluted" mode accounted for more than 80% of the BC frequency-weighted impact at this location.     

Development of a biaxial loading frame for sheet metal

Characterization of the evolving yield loci and forming limit diagrams for sheet materials under biaxial loading is necessary for the development of accurate sheet metal forming process simulations. Biaxial tension testing has been shown to have significant advantages over the current computational and experimental methods for such material characterization; however, the few commercially available loading frames are far too large and expensive to be practical for most metal forming research laboratories. In this paper, the design of a practical servohydraulic biaxial loading frame is presented. The design, control, and operation of the loading frame are discussed in detail, and experimental data is provided to validate the effectiveness of the control system with respect to specimen center shifting.     

Vivianite precipitation and phosphate sorption following iron reduction in anoxic soils

Phosphorus retention in lowland soils depends on redox conditions. The aim of this study was to evaluate how the Fe(III) reduction degree affects phosphate adsorption and precipitation. Two similarly P-saturated, ferric Fe-rich lowland soils, a sandy and a peat soil, were incubated under anaerobic conditions. M?ssbauer spectroscopy demonstrated that Fe(III) in the sandy soil was present as goethite and phyllosilicates, whereas Fe(III) in the peat soil was mainly present as polynuclear, Fe-humic complexes. Following anoxic incubation, extensive formation of Fe(II) in the solids occurred. After 100 d, the Fe(II) production reached its maximum and 34% of the citrate-bicarbonate-dithionite extractable Fe (Fe(CBD)) was reduced to Fe(II) in the sandy soil. The peat soil showed a much faster reduction of Fe(III) and the maximum reduction of 89% of Fe(CBD) was reached after 200 d. Neoformation of a metavivianite/vivianite phase under anoxic conditions was identified by X-ray diffraction in the peat. The sandy soil exhibited small changes in the point of zero net sorption (EPC?) and P(i) desorption with increasing Fe(III) reduction, whereas in the peat soil P desorption increased from 80 to 3100 μmol kg?1 and EPC? increased from 1.7 to 83 μM, after 322 d of anoxic incubation. The fast Fe(III) reduction made the peat soils particularly vulnerable to changes in redox conditions. However, the precipitation of vivianite/metavivianite minerals may control soluble P(i) concentrations to between 2 and 3 μM in the long term if the soil is not disturbed.