Mycoremediation of diclofenac using Mucor hiemalis

Diclofenac (DCF) is a pharmaceutical drug widely found in the aquatic environment, where it represents a persistent, anthropogenic hazard to all biota. Owing to the reported inefficiency of water treatment strategies to remove pharmaceuticals, the present study aimed to investigate the ability of the aquatic fungus Mucor hiemalis to take up and accumulate DCF. Cultures of M. hiemalis were exposed to varying concentrations of DCF (10, 25 and 50 µg/L) over a period of 144 h. In the presence of M. hiemalis, DCF concentrations in the media decreased by 95% within 24 h. This early removal was most likely due to extracellular metabolism of DCF, as low proportions of the pharmaceutical were found in the mycelium of the fungus, thereby excluding bioaccumulation as the main mode of removal. However, adsorption of DCF onto the surface of the M. hiemalis pellets cannot be excluded. Our study provides the first report of DCF remediation using M. hiemalis and is the first successful step towards a feasible and inexpensive bioremediation technique for DCF.     

Persistence and pathways of testosterone dissipation in agricultural soil

The persistence and pathways of dissipation of testosterone in three agricultural soils were examined in laboratory microcosm incubations at different soil moistures (1.7-39%) and temperatures (4-30 degrees C) using (14)C- and (3)H-labeled and unlabeled testosterone. Sterilized loam was also examined to assess possible abiotic pathways. Extractable (14)C decreased rapidly for all three soils at 30 degrees C with times to dissipate 50% of material (DT(50)) ranging from 8.5 to 21 h. Respired (14)CO(2) accounted for approximately 50% of the applied (14)C after 120 h. Androgenic activity of soil extracts declined faster than the extractable (14)C levels demonstrating that testosterone was not being converted to compounds with greater activity. Dissipation rates of nonvolatile, extractable (3)H in loam at 7, 15, and 39% moisture were similar, but the rate in air-dried loam (1.7% moisture) was significantly reduced. High performance liquid chromatography (HPLC) analysis of extracts of (14)C-testosterone-treated loam incubated at 30 degrees C for 6 h revealed that the (14)C was distributed among the remaining testosterone and three major metabolites (4-androstene-3,17-dione, 5alpha-androstane-3,17-dione, and 1,4-androstadiene-3,17-dione), which accounted for 48.7, 23.7, and 9.6% of the remaining (14)C, respectively. Periodic analysis of soil incubated at 23, 12, and 4 degrees C showed that the rates of testosterone dissipation and metabolite appearance and subsequent dissipation were temperature dependent with rates decreasing with decreasing temperature. In sterilized loam, 4-androstene-3,17-dione was the only metabolite detected. We conclude that testosterone is rapidly and thoroughly biodegraded in agricultural soils under a range of conditions typical of a temperate growing season and thus is unlikely to pose a long-term risk to adjacent aquatic environments.