Plant steroid hormones produced under Ni stress are involved in the regulation of metal uptake and oxidative stress in Brassica juncea L

Brassinosteroids (BRs) are involved in the amelioration of various biotic and abiotic stresses. With an aim to explore the role of BRs under heavy metal stress, plants of Brassica juncea L. were grown in pots. The plants were subjected to various concentrations of Nickel metal (0.0, 0.2, 0.4 and 0.6 mM) and harvested on 60th day in order to observe the expression of these hormones. The isolated BRs from the leaves of Brassica plants characterized by GC-MS include 24-Epibrassinolide (24-EBL), Castasterone, Dolicholide and Typhasterole. The effect of isolated 24-EBL was studied on Ni metal uptake and antioxidative defense system in 60 d old plants of Brassica. It was observed that 24-EBL significantly increased the activities of stress ameliorating enzymes and lowered the metal uptake in plants. This is the first report in B. juncea L. plants showing the expression of BRs under metal treatments and effect of the isolated 24-EBL on metal uptake and in oxidative stress management.     

ALTERNATIVES TO THE USE OF AGRICULTURAL DRAINAGE WELLS1

ABSTRACT: A large number of agricultural drainage wells (ADWs) are located in north-central Iowa. These wells permit sediments, pesticides, nitrate, and bacteria in surface and subsurface drainage water to enter regional aquifers that are currently being used for drinking-water supplies, mostly by rural families and communities. This paper reports some possible alternatives to control the entry of surface and subsurface drainage waters into groundwater systems, and describes a methodology to make comprehensive economic feasibility studies of alternative drainage outlets. The estimated cost of providing main subsurface drains varied from $220 to $960 per hectare. If the use of ADWs was completely eliminated without providing alternative drainage, it is estimated that the average annual loss to the farmers of the area would be at least $270 per hectare in reduced crop yields. Of course, losses would be weather dependent and highly variable. Management practices to reduce the pollutant load in water draining to ADWs are also discussed.     

EFFECTS OF CROPPING SYSTEMS ON NO3-N LOSSES TO TILE DRAIN1

Abstract: Diverse cropping systems can have significant impacts on nutrient losses through tile drain systems and to surface water bodies (rivers and streams). Increased transport of nitrogen to water bodies can reduce dissolved oxygen and enrich the supply of nutrients, resulting in hypoxic zones. With the objective of reducing the transport of nutrients from agricultural watersheds, long term studies (1990 to 1998) were conducted in Iowa to investigate the impact of tillage, crop rotation, and N-management practices on NO₃-N leaching losses to tile drain water. Results of these studies indicated that continuous corn production systems required higher input of nitrogen fertilizers and resulted in significantly higher NO₃-N leaching losses compared to rotated corn in plots either fertilized with manure or urea ammonium nitrate. Also, rotated corn gave higher corn yields, 8 megagrams per hectare (Mg/ha) versus 6 Mg/ha, than continuous corn. The higher N application rates resulted in increased NO₃-N concentrations in tile water. A strip cropping system with alfalfa lowered NO₃-N concentrations in tile water to less than 10 mg/l. These studies indicated that better land use practices can reduce NO₃-N leaching losses to surface and ground water systems and will help in mitigating environmental concerns of the production agriculture.     

SENSITIVITY ANALYSIS OF A DRAINAGE SIMULATION MODEL1

A sensitivity analysis of a computer model, simulating major water and nitrogen processes of a soil-water-plant-climatic system on an annual basis, was conducted to determine how the model reacts to the variations in selected hydrologic and nitrogen parameters. Two major output variables (namely, total subsurface drain volume and cumulative nitrate loss with subsurface drain water) were selected for the sensitivity analysis. Model sensitivity analysis shows that the model is most sensitive to hydrologic parameters. The model is very sensitive to variations in the initial water content in the soil profile.     

NON-STEADY SPHERICAL FLOW TO A CAVITY WELL IN AN INFINITE NON-LEAKY AQUIFER1

ABSTRACT: The non-steady drawdown distribution near a cavity well discharging from an infinite non-leaky artesian aquifer is presented. The variation of drawdown with time and distance caused by a cavity well of constant discharge in a confined aquifer of uniform thickness and uniform permeability is obtained. The solution is expressed in a series form which converges rapidly so that only two terms of the series are needed to obtain an accuracy of more than 95 percent. A simplified approach has been suggested to find the aquifer characteristics.     

Phthalates and the aquatic environment: Part II The bioconcentration and depuration of di-2-ethylhexyl phthalate (DEHP) and di-isodecyl phthalate (DIDP) in mussels (Mytilus edulis)

Mussels ($\text{Mytilus edulis}$) were exposed to di-2-ethylhexyl phthalate (DEHP) and to di-isodecyl phthalate (DIDP) over a period of 28 days. The bioconcentration factor (BCF) as measured by ¹⁴C analysis, reached estimated plateau levels corresponding to mean BCF values of approximately 2500 and 3500 for the DEHP and DIDP respectively. The mussels were then held in clean seawater for a further 14 days and ¹⁴C analysis showed a depuration half-life of approximately 3.5 days for both phthalates. During the whole 42 days of the experiment general observations on the health of the animals showed no evidence of any adverse effects.     

Phytoremediation of radiostrontium ((90)Sr) and radiocesium ((137)Cs) using giant milky weed (Calotropis gigantea R.Br.) plants

Potential of plants to remove radionuclides/toxic elements from soils and solutions can be successfully applied for removal of important radionuclides such as strontium-90 (⁹⁰Sr) and cesium-137 (¹³⁷Cs). When uptake of ¹³⁷Cs and ⁹⁰Sr by Calotropis gigantea plants incubated in distilled water spiked with the radionuclides either alone or in combination was studied, it was found to have a high efficiency for the removal of ⁹⁰Sr, with 90% being removed from solutions (5 × 10³ kBq l⁻¹) within 24 h of incubation. However, in case of ¹³⁷Cs, about 44% could be removed from solutions (5 × 10³ kBq l⁻¹) at the end of 168 h of incubation. Accumulation of ⁹⁰Sr and ¹³⁷Cs was higher in roots compared to shoots. The plants could remediate both ⁹⁰Sr and ¹³⁷Cs when they were added together to the solution. When two months old plants were incubated in low level nuclear waste, 99% of activity disappeared at the end of 15 days. The present study suggests that C. gigantea could be used as a potential candidate plant for phytoremediation of ⁹⁰Sr and ¹³⁷Cs.     

Simulating nitrate drainage losses from a Walnut Creek watershed field

This study was designed to evaluate the improved version of the Root Zone Water Quality Model (RZWQM) using 6 yr (1992-1997) of field-measured data from a field within Walnut Creek watershed located in central Iowa. Measured data included subsurface drainage flows, NO3-N concentrations and loads in subsurface drainage water, and corn (Zea mays L.) and soybean [Glycine mar (L.) Merr.] yields. The dominant soil within this field was Webster (fine-loamy, mixed, superactive, mesic Typic Endoaquolls) and cropping system was corn-soybean rotation. The model was calibrated with 1992 data and was validated with 1993 to 1997 data. Simulations of subsurface drainage flow closely matched observed data showing model efficiency of 99% (EF = 0.99), and difference (D) of 1% between measured and predicted data. The model simulated NO3-N losses with subsurface drainage water reasonably well with EF = 0.8 and D = 13%. The simulated corn grain yields were in close agreement with measured data with D < 10%. Nitrogen-scenario simulations demonstrated that corn yield response function reached a plateau when N-application rate exceeded 90 kg ha(-1). Fraction of applied N lost with subsurface drainage water varied from 7 to 16% when N-application rate varied from 30 to 180 kg ha(-1) after accounting for the nitrate loss with no-fertilizer application. These results indicate that the RZWQM has the potential to simulate the impact of N application rates on corn yields and NO3-N losses with subsurface drainage flows for agricultural fields in central Iowa.     

SPATIAL CLUSTERS OF SUBSURFACE DRAINAGE WATER NO3‐N LEACHING LOSSES1

ABSTRACT: Grouping of nitrate‐nitrogen (NO₃‐N) leaching losses from agricultural fields into spatial clusters can help determine the cause/effect relationships for their occurrence. This study was designed to investigate the spatial relationships of low, medium, and high NO₃‐N leaching losses clusters with soil and landscape attributes using cluster and discriminant analysis and the map overlay capability of a geographical information system (GIS). Field measured data of a six‐year (1993 through 1998) study on NO₃‐N leaching losses from 36 experimental fields at the Iowa State University's northeastern research center near Nashua, Iowa, were normalized on an annual basis to compare over the years. The cluster analysis resulted in the formation of three clusters based on the satisfactory evaluation criteria of pseudo‐F statistic, cubic clustering criterion, and R² values. The discriminant analysis, carried out on the basis of clusters, identified elevation and subsurface drainage as the factors that contributed significantly (p > 0.01) in discriminating among these clusters. The verification of discriminant functions developed on these factors predicted the cluster membership for all the groups with an overall accuracy of 86 percent. The map overlay analyses of GIS showed that spatial occurrence of the clusters transporting high NO₃‐N leaching losses was affected by the interaction of soil type and elevation levels.     

Status of Cadmium Concentration in Soil and Vegetable Irrigated with City Effluent

Cadmium is a toxic and carcinogenic element[1].In the1950s and 1960s,cadmium intoxication was considered to bestrictly an occupational disease.It was not until recognition of thecontribution of cadmium exposure to the etiology of Itai-itai dis-ease.It is …