Detection and Sequencing of Multiple Human Norovirus Genotypes from Imported Frozen Raspberries Linked to Outbreaks in the Province of Quebec,Canada, in 2017

Food and Environmental Virology - Human noroviruses are among the main causes of acute gastroenteritis worldwide. Frozen raspberries have been linked to several norovirus food-related outbreaks....     

Ontogenetic change in the radula of Conus magus (Gastropoda)

Specimens of the Indo-Pacific piscivorous gastropod Conus magus Linnaeus, 1758 were either collected by SCUBA in the field in Palau (1983–1984) or raised in the laboratory from egg capsules. C. magus captures adult fish with a specially modified radula tooth. Radula morphology was analyzed in a size range of individuals from 4.1 to 43.7 mm shell length, which encompassed early postmetamorphic juveniles to adults. Post-metamorphic C. magus juveniles are too small to consume fish, and all individuals below 9 mm possess a juvenile radula tooth totally different from that of the adult and resembling that of some vermivorous Conus species. The only food remains found in the digestive tracts of juveniles were the setae of syllid polychaetes. All individuals above 10.5 mm possessed adult teeth and had only fish remains in the gut. Two specimens, 10.1 and 9.2 mm in shell length, had intermediate-type radula teeth.     

Degradation of phenol and cresols at low temperatures using a suspended-carrier biofilm process

Degradation of phenol and o-, m- and p-cresol at a concentration of 150 mg l(-1) of each compound was studied in a suspended-carrier biofilm process consisting of two aerobic stages. The fungus Mortierella sarnyensis Mil'ko dominated the microflora in the first reactor, while bacteria dominated in the second reactor. The process was studied at 4, 7, 11 and 15 degrees C. The results from the experiments showed the process to be relatively efficient even at 4 degrees C. The degradation rate was 33% of that at 15 degrees C for o-cresol. Both phenol and the cresols were degraded in the first reactor and a new peak appeared in the HPLC-chromatograms indicating the formation of one or more intermediate compounds in the first stage. These compounds were however degraded to below the detection limit in the second reactor. Small new peaks appeared in the chromatograms of the outlet from the second reactor at the maximum loading rates.     

Inter- and intraspecific patterns of reproductive effort in four species of cone shells (Conus spp.)

Patterns of reproductive effort were examined both within and between 4 species of the tropical marine gastropod genus Conus from Hawaii in 1979. Caloric content was measured for the somatic tissues of female cone shells and for their egg capsule masses. These data were used to calculate (1) current reproductive effort, which was the ratio of annual energy produced as spawn to the sum of the annual energy produced as spawn plus the annual energy produced as somatic tissue; and (2) weight-specific reproductive effort, which was the ratio of annual energy produced as spawn to the total energy content of the female body just before spawning. For C. pennaceus, during the approximately 10 yr of life of a female, current reproductive effort rose from about 0.35 at 2 yr to about 0.85 at 10 yr. In contrast, weight-specific reproductive effort remained relatively constant throughout life for C. pennaceus (about 0.2 to 0.5), C. abbreviatus (about 0.2 to 0.3), C. flavidus (about 0.2 to 0.3) and C. quercinus (about 0.1 to 0.2). It is suggested that weight-specific reproductive effort may be useful as an index of the selective importance of the survival cost of present reproduction, while current reproductive effort more closely reflects the selective importance of energy allocation tradeoffs. Data on the 4 species of Conus examined support the hypothesis that reproductive effort should be positively correlated with extrinsic adult mortality and negatively correlated with the variability of juvenile survivorship. The data also suggest a possible relationship between reproductive effort and the cost of parental care.     

Adding virtual measuring stations to a network for urban air pollution mapping

Maps of pollutants concentration are usually generated by means of interpolation and extrapolation methods. The quality of the results depends mainly of the number of permanent or temporary measuring stations. This paper deals with a method for the virtual densification of the network of stations. The method creates "virtual measuring stations". It aims at improving the quality of interpolation by increasing the number of data on pollutant concentration. The virtual stations are determined by the means of a classification method applied to each pixel of the area under concern. Discriminating elements are pollutants emission classes, land cover types, urban morphological indicators created to this purpose and distance to major roads. A first implementation was made for particulate matter (PM) for the city of Strasbourg (France) using thin-plates spline interpolation method in Arcview 9 GIS. The relative Root Mean Square Error decreases from 49% for five input stations down to 15% for the virtual stations.     

Assessing organic contaminant fluxes from contaminated sediments following dam removal in an urbanized river

In this study, methods and approaches were developed and tested to assess changes in contaminant fluxes resulting from dam removal in a riverine system. Sediment traps and passive samplers were deployed to measure particulate and dissolved polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) in the water column prior to and following removal of a small, low-head dam in the Pawtuxet River, an urbanized river located in Cranston, RI, USA. During the study, concentrations of particulate and dissolved PAHs ranged from 21.5 to 103 μg/g and from 68 to 164 ng/L, respectively. Overall, temporal trends of PAHs showed no increases in either dissolved or particulate phases following removal of the dam. Dissolved concentrations of PCBs were very low, remaining below 1.72 ng/L at all sites. Particulate PCB concentrations across sites and time showed slightly greater variability, ranging from 80 to 469 ng/g, but with no indication that dam removal influenced any increases. Particulate PAHs and PCBs were sampled continuously at the site located below the dam and did not show sustained increases in concentration resulting from dam removal. The employment of passive sampling technology and sediment traps was highly effective in monitoring the concentrations and flux of contaminants moving through the river system. Variations in river flow had no effect on the concentration of contaminants in the dissolved or particulate phases, but did influence the flux rate of contaminants exiting the river. Overall, dam removal did not cause measurable sediment disturbance or increase the concentration or fluxes of dissolved or particulate PAHs and PCBs. This is due in large part to low volumes of impounded sediment residing above the dam and highly armored sediments in the river channel, which limited erosion. Results from this study will be used to improve methods and approaches that assess the short- and long-term impacts ecological restoration activities such as dam removal have on the release and transport of sediment-bound contaminants.     

Larval biology of six species of the genus Conus (Gastropoda: Toxoglossa) in Hawaii,USA

Among the Hawaiian species in the genus Conus, larval life histories range from long-term planktotrophy to nonpelagic lecithotrophy. Prehatching developmental time, hatching size, pelagic period and total prejuvenile developmental time are all significantly correlated with egg size, while settling size is correlated with none of these larval characteristics. Therefore, larval life history patterns in the genus Conus appear to be determined by 2 sets of selective forces: the first influencing egg size, and the second stipulating settling size. As egg sizes become larger, hatching sizes and prehatching developmental time increase while pelagic periods and total prejuvenile developmental times decrease. Settling size appears to be related to water depth, but food requirements of newly metamorphosed juveniles may also be important.     

Limitations of reverse polyethylene samplers (RePES) for evaluating toxicity of field contaminated sediments

Passive samplers are used to measure dissolved nonionic organic contaminants (NOCs) in environmental media. More recently, reverse polyethylene samplers (RePES) have been used with spiked sediments to recreate interstitial water exposure concentrations and observed toxicity. In the present study, RePES were used with field contaminated sediments. The RePES was not capable of recreating the pattern of toxicity with the amphipod and mysid observed with intact field sediments. Decreased survival in the RePES exposures as compared to the whole sediment exposures was most likely caused by an overexposure to NOCs due to a lack of surrogate black carbon in the RePES system. As an alternative, aqueous phase studies were performed in which polyethylene was allowed to equilibrate with slurries of intact sediments for 3 weeks. Three weeks was found to be an insufficient amount of time for the polyethylene to equilibrate with the sediment. An additional study demonstrated 3 months was sufficient for lower contaminant concentrations, but might not be an adequate amount of time for more highly contaminated sediments. The aqueous phase transfer approach may be useful if equilibration is sufficiently long, although this length of time may be impractical for use in certain applications, such as toxicity identification evaluations (TIEs).     

Influences, practices and opportunities for environmental supply chain management in Nova Scotia SMEs

Executives are increasingly paying attention to the importance of supply chain management [Lambert DM, Cooper MC. Issues in supply chain management. Industrial Marketing Management 2000;29(1):65–83; Hagelaar GJLF, van der Vorst JGAJ. Environmental supply chain management: using life cycle assessment to structure supply chains. International Food and Agribusiness Management Review 2002;4:399–412]. The management of the supply chain can be a central aspect of a company's competitive advantage [Preuss L. Rhetoric and reality of corporate greening: a view from the supply chain management function. Business Strategy and the Environment 2005;14:123–139]. SCM also offers considerable opportunities to reduce a company's environmental impact. The supply chains of three small and medium enterprises (SMEs) operating in the Burnside Industrial Park in Nova Scotia, Canada were investigated to explore the opportunities to improve environmental performance of SMEs linked in supply chains. This study confirmed that time and, to a lesser degree, financial resources to address solid waste and energy issues are the greatest limiting factors. Small suppliers, and even to a certain degree medium-sized enterprises, have difficulties in allocating resources to initiatives that are not viewed as directly related to their core function, namely manufacturing the product or providing the service. This study clearly demonstrated that opportunities exist to reduce greenhouse gas emissions and solid waste. Although, the benefits that would be gained from the implementation of any of the individual actions in the supply chains explored in this study are individually small, the cumulative benefits that could be achieved among supply chains and within industrial parks are substantial, given the number of small- and medium-sized enterprises.     

对美国格林威治湾底栖动物造成危害的潜在胁迫因子的诊断

格林威治湾是纳拉甘西特湾一处受到多种影响因子作用的城市化形成港湾。本研究鉴定了对于格林威治湾底栖动物的重要影响因素。首先利用现存数据与信息来验证水体受损情况。其次确定了影响来源，影响因素和具体影响的存在。继而探究了来源，影响因素和具体影响之间的关系。这使我们能够鉴定出最可能危害水体的影响因素。本研究评估了化学制品、营养物质和悬浮沉积物3类污染物。这种证据权重的方法表明格林威治湾主要受到富营养化相关的影响因素危害。格林威治湾的沉积物富含碳，低溶解氧浓度的情况十分常见，尤其在格林威治湾西部。底栖生物群落出现发育不良的情况，与在氧气不足情况下的预测结果吻合。尽管我们的分析表明环境中的污染物浓度可能导致有害结果的出现，我们并未在结果中检出毒性。这是由于沉积物中存在大量的有机物，这些有机物限制了污染物的生物可利用性。我们的分析还表明悬浮沉积物的影响对于湾区的大部分区域而言几乎不存在。本分析展示了诊断法步骤可以用于组织和评估影响格林威治湾生态健康的潜在影响因素。诊断法步骤对于管理受到多重因素影响的水体非常有用。
精选自Marguerite Pelletier, Kay Ho, Mark Cantwell, Monique Perron, Kenneth Rocha, Robert M. Burgess, Roxanne Johnson, Kenneth Perez, John Cardin, Michael A. Charpentier. Diagnosis of potential stressors adversely affecting benthic invertebrate communities in Greenwich Bay, Rhode Island, USA. Environmental Toxicology and Chemistry: Volume 36, Issue 2, pages 449–462, July 2017. DOI: 10.1002/etc.3562
详情请见http://onlinelibrary.wiley.com/wol1/doi/10.1002/etc.3562/full