Diversity of bacterial isolates during full scale rotary drum composting

Bacterial diversity of full scale rotary drum composter from biodegradable organic waste samples were analyzed through two different approaches, i.e., Culture dependent and independent techniques. Culture-dependent enumerations for indigenous population of bacterial isolates mainly total heterotrophic bacteria (Bacillus species, Pseudomonas species and Enterobacter species), Fecal Coliforms, Fecal Streptococci, Escherichia coli, Salmonella species and Shigella species showed reduction during the composting period. On the other hand, Culture-independent method using PCR amplification of specific 16S rRNA sequences identified the presence of Acinetobacter species, Actinobacteria species, Bacillus species, Clostridium species, Hydrogenophaga species, Butyrivibrio species, Pedobacter species, Empedobactor species and Flavobacterium species by sequences clustering in the phylogenetic tree. Furthermore, correlating physico-chemical analysis of samples with bacterial diversity revealed the bacterial communities have undergone changes, possibly linked to the variations in temperature and availability of new metabolic substrates while decomposing organics at different stages of composting.     

Wastewater management for hazardous chemicals ‐ disposal of ammonium perchlorate wastes from propellant production for space vehicles

An attempt to depict the wastewater treatment system for the production of ammonium perchlorate, a component of solid propellant used in space vehicles, has been made. The waste‐water management system uses solar ponds and seawater dilution for the retention and disposal of the undesirable constituents of the wastewater. The details of the wastewater generation and its treatment are highlighted in this paper.     

Real-time quantification of mcrA, pmoA for methanogen, methanotroph estimations during composting

Composting is the controlled biological decomposition of organic matter by microorganisms during predominantly aerobic conditions. It is being increasingly adopted due to its benefits in nutrient recycling, soil reclamation, and urban land use. However, it poses an environmental concern related to its contribution to greenhouse gas production. During composting, activities of methanogenic and methanotrophic communities influence the net methane (CH4) release into the atmosphere. Using quantitative polymerase chain reaction (qPCR), this study was aimed at assessing the changes in the methyl-coenzyme M reductase (mcrA) and particulate methane monooxygenase (pmoA) copy numbers for estimation of methanogenic and methanotrophic communities, respectively. Open-windrow composting of beef cattle (Bos Taurus L.) manure with temperatures reaching > 55 degrees C was effective indegrading commensal Escherichia coli within the first week. Quantification of community DNA revealed significant differences in mcrA and pmoA copy numbers between top and middle sections. Consistent mcrA copy numbers (7.07 to 8.69 log copy number g(-1)) were detected throughout the 15-wk composting period. However, pmoA copy number varied significantly over time, with higher values during Week 0 and 1 (6.31 and 5.41 log copy number g(-1), respectively) and the lowest at Week 11 (1.6 log copy number g(-1)). Net surface CH4 emissions over the 15-wk period were correlated with higher mcrA copy number. Higher net ratio of mrA: pmoA copy numbers was observed when surface CH4 flux was high. Our results indicate that mcrA and pmoA copy numbers vary during composting and that methanogen and methanotroph populations need to be examined in conjunction with net CH4 emissions from open-windrow composting of cattle feedlot manure.     

Laboratory and Field Assessment of Arsenic Testing Field Kits in Bangladesh and West Bengal, India

High concentrations of arsenic in ground waters in West Bengal and Bangladeshhave become a major cause for concern in recent years. Given the enormity and the severity of the problemof arsenic poisoning, a task of evaluating the commercially available arsenic detection field kits for their capabilities was undertaken. In the light of the findings, generic specificationswere recommended which could form the basis forindigenous manufacture of these kits in the arsenic affected countries. This article presents the results of the laboratory and fieldevaluation conducted in Bangladesh and West Bengalof five arsenic testing field kits. The salient features of the kits, their merits and limitationshave been brought out. Based on the criteria of kitdesign, quality of chemicals used, colourcomparator charts, detection range, time required for analysis, cost etc., a comparative ranking ofthe kits has been made to facilitate the choice of the kit to meet specific requirements.     

Comparative studies on biomass productivity and lipid content of a novel blue-green algae during autotrophic and heterotrophic growth

Environmental Science and Pollution Research - Algae have long been acclaimed as the attractive renewable source for generating third-generation biofuels, particularly biodiesel. Under the present...     

Carbon storage versus fossil fuel substitution: a climate change mitigation option for two different land use categories based on short and long rotation forestry in India

Short rotation bioenergy crops for energy production are considered an effective means to mitigate the greenhouse effect, mainly due to their ability to substitute fossil fuels. Alternatively, carbon can be sequestered and stored in the living biomass. This paper compares the two land use categories (forest land and non-forest land) for two management practices (short rotation vs. long rotation) to study mitigation potential of afforestation and fossil fuel substitution as compared to carbon storage. Significant carbon benefit can be obtained in the long run from using lands for growing short rotation energy crops and substituting fossil fuels by the biomass thus produced, as opposed to sequestering carbon in the biomass of the trees. When growth rates are high and harvest is used in a sustainable manner (i.e., replanting after every harvest), the opportunities for net carbon reductions appear to be fossil fuel substitution, rather than storage in ecosystem biomass. Our results suggest that at year 100 a total of 216 Mg C ha⁻¹ is sequestered for afforestation/reforestation using long rotation sal (Shorea robusta Gaertn.f) species, as opposed to offset of 412 Mg C ha⁻¹ for carbon storage and fossil fuel substitution for short rotation poplar (Populus Deltoides Marsh) plantations. The bioenergy option results in a continuous stream of about 3 Mg C ha⁻¹ yr⁻¹ of carbon benefits per year on forest land and 4 Mg C ha⁻¹ yr⁻¹ on non-forest land. Earlier studies have shown that in India waste land availability for establishing energy plantations is in the range of 9.6 to 36.5 Mha. Thus, using the 758 Tg biomass per year generated from 9.6 Mha waste land gives a mitigation potential in the range of 227 to 303 Tg C per year for carbon storage and fossil fuel substitution from poplar plantation for substituting coal based power generation. Depending upon the land availability for plantation, the potential for energy generation is in the range of 11,370 PJ, possibly amounting to a bioenergy supply of 43% of the total projected energy consumption in 2015. Further studies are needed to estimate the mitigation potential of other species with different productivities for overall estimation of the economic feasibility and social acceptability in a tropical country like India.     

Biosynthesis and effects of copper nanoparticles on plants

Copper nanoparticles have improved properties compared to the bulk copper material. Copper nanoparticles indeed find applications in gas sensors, heat transfer fluids, catalysis, solar energy and batteries. Antibacterial and antifungal activities of copper nanoparticles find applications in the agriculture and healthcare sectors. Nonetheless, careless use of copper nanoparticles may cause environmental pollution and health effects. Here we review the biosynthesis of copper nanoparticles using plant materials, named phytosynthesis, and micro-organisms. We also discuss the effect of copper nanoparticles on crops and pathogenic micro-organisms. Copper nanoparticles varying in sizes from 5 to 295 nm have been synthesized using leaf extracts and latex from plants, and using bacteria and fungi. Biosynthesized copper nanoparticles show good antimicrobial activity inhibiting the growth of pathogenic bacteria and pathogenic fungi. Copper nanoparticles enhance the germination and growth of some plants at lower concentrations, whereas high concentrations result in retarded growth.