Trisomy 12 mosaicism in phenotypically normal fetuses following prenatal detection

We report three cases of amniocentesis in which mosaicism for trisomy 12 was detected in two or more independent cultures. The parents elected to terminate the pregnancy in all three cases. Follow-up studies in two of the cases confirmed the mosaicism in fetal tissues (in subcutaneous tissue in one case; in fetal lung in the other), but not in blood. No fetal anomalies were evident by ultrasound or at autopsy. These results along with other reported cases demonstrate the difficulty in counselling for mosaic trisomy 12.     

Seasonal variations in cholinesterase activity, nerve conduction velocity and lung function among sprayers exposed to mixture of pesticides

Pesticide spraying operation is associated with the increased risk of adverse health effects among sprayers who do not follow safe farm work practices. A study was conducted among pesticide sprayers in North India to evaluate the clinical and subclinical variations in their vital health parameters before and after the pesticide spraying season. Blood cholinesterase levels, pulmonary function test, nerve conduction velocity and self-reported symptoms were studied among 18 eligible and consenting male sprayers. Mean acetylcholinesterase activity was reduced by 55 % in the post-exposure assessment (P?<?0.001) as compared to pre-exposure levels. Mean forced expiratory volume in 1 s was 20 % lower in the post-exposure assessment as compared to the pre-exposure level (P?<?0.05). No significant change was observed in the motor and sensory nerve conduction velocity in the median nerve of sprayers before and after the spraying activity. Also, no significant variation was observed with respect to self-reported symptoms except weakness in arms and legs (P?<?0.05). The significant decline in lung function and acetylcholinesterase level after pesticide exposure reflects the strongly negative effect of exposure to pesticides during spraying activity. More longitudinal studies among pesticide sprayers must be undertaken to further substantiate the cause–effect relationship between pesticide exposure and its subclinical effects. There is a strong necessity to minimise the exposure through the use of personal protective equipment in pesticide sprayers.     

Rainwater conservation and recycling by optimal size on-farm reservoir

Hydrologic and economic analysis of the on-farm reservoir (OFR) was carried out in rainfed rice–mustard cropping systems in Eastern India followed by 2 years of field experiments in 1999 and 2000. The average contribution (average of 2 years) of direct rainfall and surface runoff from the diked crop fields contributed, respectively, about 79.5 and 20.5% to the total OFR inflow. The average contribution of evaporation loss, seepage and percolation loss and supplemental irrigation from the OFR contributed, respectively, about 10.0, 31.2 and 58.8% to the total OFR outflow. There was an average increase of rice yield of 44.0% over the rainfed rice because of application of 8.4 cm supplemental irrigation from the OFR. Thus, with an application of 4.5 cm supplemental irrigation from the OFR, 15.40% increase of mustard yield was recorded in 1999. Economic analysis indicated average net profit of Rs. 700 (US$ 1 = Rupees (Rs.) 44.75 in Indian currency) from a farm area of 800 m². Average values of benefit–cost ratio, internal rate of return and pay back period of the OFR irrigation system were evaluated as 1.17, 14.8%, and 16 years, respectively. The study reveals that the OFR irrigation in small landholders is economically feasible system for rainwater harvesting and providing supplemental irrigation in rainfed farming system.     

Fetal karyotype following ascertainment of fetal anomalies by ultrasound

Data pooled from contributors to a Registry for Cytogenetic Abnormalities and PKU (ReCAP) shows an unbalanced chromosome abnormality rate of 27 per cent (29 fetuses) for 107 fetuses with ultrasonically diagnosed fetal anomalies. Of the abnormal, 12 were trisomic, 6 were monosomy X and 6 were structural abnormalities, 4 were mosaics and one triploid.