Effect of sulphadimethoxine contamination on barley (Hordeum distichum L., Poaceae, Liliposida)

Animal wastes from intensive farming are generally collected for field fertilisation. They may contain drugs that can become soil pollutants. To evaluate the possible effects of such contamination in terrestrial systems, sulphadimethoxine has been subjected to laboratory tests (in vitro, synthetic medium, and soil) using seeds of barley (Hordeum distichum L.). The drug suppressed normal post-germinative development and growth of roots and leaves in both test conditions; this effect was dependent on the bioaccumulation rate, which was higher on synthetic medium than in soil. Bioaccumulation was higher in roots than foliage and this was markedly evident in soil and, in particular, in soils with a low humus content. The environmental risk of sludge application on soils and the possible contamination of food chains are discussed.     

Phytotoxicity to and uptake of flumequine used in intensive aquaculture on the aquatic weed, Lythrum salicaria L

Phytotoxicity of Flumequine on the aquatic weed Lythrum salicaria L. was determined by two laboratory models: a single concentration test, by which the effects of 100 mg l-1 were evaluated after 10, 20, 30 days and a multiple concentration test, by which the effects of 5000-1000-500-100-50 micrograms l-1 were evaluated after 35-day exposure. 100 mg l-1 are highly toxic and significantly decrease the growth of plants; this effect increases with time. Concentrations between 5000 and 50 micrograms l-1 induced hormesis in plants, by significantly increasing mean number and dimension of leaves and secondary roots. The effect is the highest at 50 micrograms l-1 and decreases with increase in concentration. Both toxic effect and hormesis can be related to plant drug uptake, quite high, in the order of micrograms g-1. The ecological implication of Flumequine contamination in aquatic environments and the possible use of Lythrum salicaria for bioremediation and/or monitoring technique are discussed.     

Higher sorption of arsenate versus arsenite on amorphous Al-oxide, effect of ligands

Arsenic pollution is currently a major health issue because As is toxic for human beings, animals, and plants. Knowledge of As mobility is therefore important to assess health risk. The sorption of arsenite and arsenate on metal oxides in the presence of various anionic ligands is closely linked to the mobility, bioavailability, and risk. It was reported that the sorption mechanisms and characteristics of arsenite and arsenate on Al-oxides were different from that on Fe-oxides. Previous work reports the sorption of arsenite and arsenate on Fe-oxides in the presence of ligands. Whereas there is few knowledge on the sorption of arsenite and arsenate by Al-oxides in the presence of ligands. Here, we studied the sorption of arsenite and arsenate on amorphous Al-oxide by batch experiments. We tested the effect of organic ligands: oxalate, malate, tartrate, citrate; and inorganic ligands: sulfate, phosphate, selenate, selenite. Results show that amorphous Al-oxide has more sorption affinity for arsenate than arsenite. The inhibition of As sorption by ligands at pH 6 is higher for arsenite than arsenate. For arsenite, the As sorption inhibition decreases in the order phosphate, citrate, malate, selenite, oxalate, tartrate, sulfate, and selenate. For arsenate, the As sorption inhibition decreases in the order phosphate, malate, citrate, selenite, tartrate, oxalate, sulfate, and selenate.     

Phytotoxicity to and uptake of enrofloxacin in crop plants

Phytotoxicity of enrofloxacin on crop plants Cucumis sativus, Lactuca sativa, Phaseolus vulgaris and Raphanus sativus was determined in a laboratory model: the effect of 50, 100 and 5000 microgl(-1) were evaluated after 30 days exposure by measuring post-germinative growth of primary root, hypocotyl, cotyledons and leaves. Concentrations between 50 and 5000 microgl(-1) induced both toxic effect and hormesis in plants, by significantly modifying both length of primary root, hypocotyl, cotyledons and the number/length of leaves. A toxic effect is induced by high concentration (5000 microgl(-1)), while hormesis occurs at low concentrations (50 and 100 microgl(-1)). A continuum between toxic effect and hormesis is found in the four plant species. Both toxic effect and hormesis can be related to an efficient plant drug uptake, in the order of microgg(-1). Plants are able to metabolize enrofloxacin into ciprofloxacin, as also happens in animals; Cucumis, Lactuca and Phaseolus biologically convert about one quarter of stored enrofloxacin. The ecological implication of enrofloxacin contamination in terrestrial environments is discussed.     

Polymerization of humic substances by an enzyme-catalyzed oxidative coupling

 A novel understanding of the structural features of humic substances supports the self-assembly supramolecular association of relatively small molecules rather than their polymeric nature. An increase in the conformational stability of humus may thus be achieved through promotion of intermolecular covalent bondings between heterogeneous humic molecules by an enzyme-catalyzed oxidative reaction. We present evidence from high performance size exclusion chromatography (HPSEC) and diffuse reflectance infrared spectrometry (DRIFT) that oxidation of a humic material catalyzed by horseradish peroxidase stabilizes the humic structure by the formation of aryl and alkyl ethers and permanently enhances its molecular size. Received: 15 May 2000 / Accepted in revised form: 14 July 2000     

Extending large-scale forest inventories to assess urban forests

Urban areas are continuously expanding today, extending their influence on an increasingly large proportion of woods and trees located in or nearby urban and urbanizing areas, the so-called urban forests. Although these forests have the potential for significantly improving the quality the urban environment and the well-being of the urban population, data to quantify the extent and characteristics of urban forests are still lacking or fragmentary on a large scale. In this regard, an expansion of the domain of multipurpose forest inventories like National Forest Inventories (NFIs) towards urban forests would be required. To this end, it would be convenient to exploit the same sampling scheme applied in NFIs to assess the basic features of urban forests. This paper considers approximately unbiased estimators of abundance and coverage of urban forests, together with estimators of the corresponding variances, which can be achieved from the first phase of most large-scale forest inventories. A simulation study is carried out in order to check the performance of the considered estimators under various situations involving the spatial distribution of the urban forests over the study area. An application is worked out on the data from the Italian NFI.     

Sorption of Cu, Pb and Cr on Na-montmorillonite: competition and effect of major elements

The competitive sorption among Cu, Pb and Cr in ternary system on Na-montmorillonite at pH 3.5, 4.5 and 5.5 and at different heavy metal concentrations, and the effect of varying concentrations of Al, Fe, Ca and Mg on the sorption of heavy metals were studied. Competitive sorption of Cu, Pb and Cr in ternary system on montmorillonite followed the sequence of Cr ? Cu > Pb. Moreover, the competition was weakened by the increase of pH while was intensified by the increase of heavy metal concentration. The sorption of heavy metal on montmorillonite was inhibited by the presence of Ca and Mg, while Al and Fe showed different patterns in affecting heavy metal sorption. Aluminum and Fe generally inhibited the sorption of heavy metal when the pH and/or concentration of major elements were relatively low. However, promoting effects on heavy metal sorption by Al and Fe were found at relatively high pH and/or great concentration of major elements. The inhibition of major elements on heavy metal sorption generally followed the order of Al > Fe > Ca ? Mg, while Fe was more efficient than Al in promoting the sorption of heavy metals. These findings are of fundamental significance for evaluating the mobility of heavy metals in polluted environments.     

Laboratory models to evaluate phytotoxicity of sulphadimethoxine on terrestrial plants

Antimicrobial drugs soil contamination is a possible side effect of spreading animal wastes from intensive farming on arable land: waste often contain persistent drugs (like sulphonamides) that pollute soil. In laboratory tests different crop plants and weeds demonstrated, on both synthetic medium and soil, toxicity and sulphadimethoxine bioaccumulation. These data suggest potential adverse implications for organisms higher up the food net, including humans. The increasingly used technique of'soil top dressing will impose the monitoring of antimicrobial contamination in arable lands and their crops, to protect natural ecosystems and consumer. A possible tool for this monitoring is the use of weeds that can constitute a “mesh” where antimicrobial contamination can be detected.