Equilibrium and kinetic studies on biosorption of Hg(II), Cd(II) and Pb(II) ions onto microalgae Chlamydomonas reinhardtii

The microalgae Chlamydomonas reinhardtii was used for the biosorption of Hg(II), Cd(II) and Pb(II) ions. The maximum adsorption of Hg(II) and Cd(II) ions on Chlamydomonas reinhardtii biomass was observed at pH 6.0 and the corresponding value for Pb(II) ions was 5.0. The biosorption of Hg(II), Cd(II) and Pb(II) ions by microalgae biomass increased as the initial concentration of Hg(II), Cd(II) and Pb(II) ions increased in the biosorption medium. The maximum biosorption capacities of microalgae for Hg(II), Cd(II) and Pb(II) ions were 72.2+/-0.67, 42.6+/-0.54 and 96.3+/-0.86 mg/g dry biomass, respectively. The affinity order for algal biomass was Pb(II)>Hg(II)>Cd(II). FT-IR analysis of algal biomass revealed the presence of amino, carboxyl, hydroxyl and carbonyl groups, which were responsible for biosorption of metal ions. Biosorption equilibrium was established in about 60 min and the equilibrium was well described by the Freundlich biosorption isotherms. Temperature change in the range of 5-35 degrees C did not affect the biosorption capacity. The microalgae could be regenerated using 0.1 M HCl, with up to 98% recovery, which allowed the reuse of the biomass in six biosorption-desorption cycles without any considerable loss of biosorption capacity.     

Mineral contents of seed and seed oils of Capparis species growing wild in Turkey

The mineral contents of seed and seed oils of Capparis species growing wild in Turkey were established by inductively coupled plasma–atomic emission spectrometry. Capparis spinosa var. spinosa (2010) and Capparis ovata var. canescens variety (2009) were determined to be rich in terms of mineral matter as 19,514.60 and 16,995.92 ppm as a total, respectively. C. spinosa var. spinosa collected from Mu?la-Milas region (2009) had the highest amount of Ca with 1,010.67 ppm in C. spinosa species and in C. ovata species. C. ovata var. canescens collected from Ankara-Beypazar? (2010) region had the highest amount of Ca with 833.92 ppm Ca amount in C. spinosa var. spinosa, inermis, herbaceae seeds decreased in 2010. C. spinosa var. inermis collected from Antalya-Serik (2010) in C. spinosa species had rich amount of Ca with 123.78 ppm and C. ovata var. palaestina seed oils collected from Mardin-Savur region (2009) had rich amount of Ca with 253.71 ppm in C. ovata species. The oil of C. spinosa var. herbaceae variety collected from Mardin-Midyat region (2010) was determined to have the highest major mineral matter (Ca, K, Mg, Na, and P) with 1,424.37 ppm in C. spinosa species. It was also determined that as a result, caper seed and oils were found to be important sources of nutrients and essential elements.     

Protective effects of melatonin and vitamin E in acetamiprid-induced nephrotoxicity

Environmental Science and Pollution Research - Investigation of probable toxic effects of acetamiprid (ACMP) on kidney and comparative analysis of the probable protective effects of vitamin E and...     

Spatial analysis and facility characteristics of outdoor recreational areas in Istanbul

This article reports the results of a study that explored whether outdoor recreational areas are sufficient in Istanbul in terms of their surface area and facility characteristics. All the municipalities in 32 subprovinces of Istanbul were sent a survey in 2007 and asked to prepare a list of their outdoor recreational areas including their names, addresses, size, and facilities. All the data collected from the municipalities were used to create a GIS-based inventory by using GIS and remote sensing. As the study revealed, the outdoor recreational areas in Istanbul are far behind meeting the recreational needs of the residents in terms of area per person and facility characteristics. There are 2,470 areas which were dedicated to outdoor recreational activities in Istanbul. Total surface area of all these outdoor recreational areas is 19,2 sq kilometers; this means 1.52 m² recreational area per person in the city. This value is very low when compared to that of many cities in Europe and USA. As the study also revealed, the majority of outdoor recreational areas in Istanbul are poor in facility. Majority of the existing outdoor recreational areas are small and do not provide the public with many opportunities to engage in different outdoor activities. A more sustainable and efficient recreational plan is needed in Istanbul to meet the various recreational needs of its residents.     

Denaturing high-performance liquid chromatography (DHPLC)-based prenatal diagnosis for tuberous sclerosis

Tuberous sclerosis (TSC) is a frequent autosomal-dominant condition (affecting 1 in 6000 individuals) caused by various mutations in either the hamartin (TSC1) or the tuberin gene (TSC2). This allelic and non-allelic heterogeneity makes genetic counseling and prenatal diagnosis difficult, especially as a significant proportion of TSC cases are due to de novo mutations. For this reason the identification of the disease causing mutation is mandatory for accurate counseling, yet current mutation detection methods such as single-strand conformation polymorphism (SSCP) or denaturing gradient gel electrophoresis (DGGE) are labor intensive with limited detection efficiency. Denaturing high-performance liquid chromatography (DHPLC) is a high-throughput, semi-automated mutation detection system with a reported mutation detection rate close to 100% for PCR fragments of up to 800 bp. We used a recently described DHPLC assay allowing the efficient detection of mutations in TSC1 to analyze the DNA extracted from a chorion villus sample in order to perform a prenatal diagnosis for TSC. The fetus was found not to have inherited the deleterious mutation and the DHPLC diagnosis was confirmed by haplotype analysis. This represents the first DHPLC-based prenatal diagnosis of a genetic disease. Copyright © 2001 John Wiley & Sons, Ltd.     

Development of an efficient and sensitive analytical method for the determination of copper at trace levels by slotted quartz tube atomic absorption spectrometry after vortex-assisted dispersive liquid-liquid microextraction in biota and water samples using a novel ligand

This study describes the determination of trace levels of copper by slotted quartz tube atomic absorption spectrometry after dispersive liquid-liquid microextraction. A ligand synthesized from the reaction of salicylaldehyde and 1-naphthylamine was used to form coordinate copper complex prior to extraction. All parameters that influence the output of complex formation, extraction, and instrumental measurement were optimized to enhance the absorbance signal of copper. Under the optimum conditions, about 104-fold enhancement in sensitivity was recorded over the conventional flame atomic absorption spectrometer, corresponding to a 0.51 ng/mL detection limit. The percent relative standard deviation calculated for the lowest concentration (4.8%) indicated high precision for the experimental procedure. Accuracy and applicability of the optimum method were determined by performing spiked recovery tests on urine, lake water, and mineral water samples. Satisfactory recovery results were obtained between 82.2 and 106.3% at four different concentrations. Matrix matching method was also performed to increase the accuracy of quantification, and the percent recovery calculated for 175 ng/mL was 105.14%.     

Determination of protective effect of carob (Ceratonia siliqua L.) extract against cobalt(II) nitrate-induced toxicity

Environmental Science and Pollution Research - Cobalt (Co) is widely used in many industrial fields such as batteries and paints. Cobalt, a dangerous heavy metal, can be found in high...