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The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems 总被引:3,自引:0,他引:3
Kratasyuk VA Esimbekova EN Gladyshev MI Khromichek EB Kuznetsov AM Ivanova EA 《Chemosphere》2001,42(8):909-915
A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductase-luciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and “bloom” of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the “bloom” of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies. 相似文献
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