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The biomixture is a principal element controlling the degradation efficacy of the biobed. The maturity of the biomixture used in the biobed affects its overall performance of the biobed, but this is not well studied yet. The aim of this research was to evaluate the effect of using a typical composition of Swedish biomixture at different maturity stages on the degradation of chlorpyrifos. Tests were made using biomixture at three maturity stages: 0 d (BC0), 15 d (BC15) and 30 d (BC30); chlorpyrifos was added to the biobeds at final concentration of 200, 320 and 480 mg kg−1. Chlorpyrifos degradation in the biomixture was monitored over time. Formation of TCP (3,5,6-trichloro-2-pyrinidol) was also quantified, and hydrolytic and phenoloxidase activities measured. The biomixture efficiently degraded chlorpyrifos (degradation efficiency >50%) in all the evaluated maturity stages. However, chlorpyrifos degradation decreased with increasing concentrations of the pesticide. TCP formation occurred in all biomixtures, but a major accumulation was observed in BC30. Significant differences were found in both phenoloxidase and hydrolytic activities in the three maturity stages of biomixture evaluated. Also, these two biological activities were affected by the increase in pesticide concentration. In conclusion, our results demonstrated that chlorpyrifos can be degraded efficiently in all the evaluated maturity stages. 相似文献
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G.R. Tortella R.A. Mella-Herrera D.Z. Sousa O. Rubilar G. Briceño L. Parra M.C. Diez 《Chemosphere》2013
The impact of repeated carbendazim (CARB) applications on the extent of CARB dissipation, the microbial diversity, the community level physiological profile (CLPP), and the enzymatic activity within the biomixture of an on-farm biopurification system was evaluated. After three successive CARB applications, the CARB dissipation efficiency was high; the efficiency of dissipation was 87%, 94% and 96% after each application, respectively. Although microbial enzymatic activity was affected significantly by CARB application, it could recover after each CARB pulse. Likewise, the numbers of cultivable bacteria, fungi and actinomycetes (as measured in CFUs) were slightly affected by the addition of CARB, but the inhibitory effect of the pesticide application was temporary. Denaturing gradient gel electrophoresis (DGGE) and Biolog Ecoplate assays demonstrated that the microbial populations remained relatively stable over time when compared to the control. The results obtained herein therefore demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system. 相似文献
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Hoffmann Nicolás Fincheira Paola Tortella Gonzalo Rubilar Olga 《Environmental science and pollution research international》2022,29(55):82619-82631
Environmental Science and Pollution Research - Anaerobic digestion (AD) is the most widely used technology for organic matter treatment. However, multiple types of research have reported on... 相似文献
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Currently there is a growing interest in developing novel bioproducts and biomaterials derived from renewable sources that can reduce the dependence on fossil fuel feedstock. In this study a protein concentrate from microalgae Botryococcus braunii residual biomass (MPC) from a biorefinery process was used as a biopolymer to develop ultrafine fibers by electrospinning. Experiments were designed to study the effect of different formulations of MPC, poly(ethylene oxide) and pH on morphology and diameter of fibers. The results indicated MPC fibers from acidic solutions prepared at pH 1 had smoother and smaller diameter than those fibers from alkaline solutions (pH 12). Moreover, under the conditions studied, it was conclude that pH and the concentration of MPC were the most significant factors in determining the diameter and morphology of the fibers obtained. Fourier transform infrared analysis showed there is a slight frequency shift for the secondary structure of MPC as induced by change in pH of the polymer solutions. Likely this change in the protein structure improved the physical chain entanglement in the polymer blend. The results of this work revealed a potential to develop fibers from MPC from residual biomass by a promising technique that may find many end-use applications. 相似文献
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Rodrigo Navia Olga Rubilar M Cristina Diez Karl-Heinz Schmidt Gerhard Behrendt Karl E Lorber 《Waste management & research》2007,25(2):119-129
The volcanic soils of southern Chile have demonstrated a high capacity to adsorb environmental pollutants, but for an industrial application, a stable solid material is necessary. The objective of this work was to produce a stable ceramic material through a process involving volcanic soil-polyurethane foam produced with recycled polyethylene terephthalate (PET)-polyols, and further thermal treatment. The selected foam formulation with 35.4% volcanic soil (< 63 microm) seems to be the most suitable for thermal treatment, with temperature steps at 700, 850, 1000 and 1200 degrees C. The porous ceramic material obtained has a stable solid form and an improved chlorophenols adsorption capacity (comparable to natural zeolites) that makes it suitable for advanced wastewater treatment and landfill leachate depuration. 相似文献
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Catalina T. Pastor-de-Ward Tamara Rubilar María E. Díaz-de-Vivar Ximena Gonzalez-Pisani Erica Zarate Marina Kroeck Enrique Morsan 《Marine Biology》2007,151(1):205-217
The starfish Cosmasterias lurida was sampled from the pier piles of Almirante Storni in the Golfo Nuevo, northern Patagonia, from November 2001 to November
2003. In this anthropogenically modified site there is an unusually abundant food supply of mussels and large aggregations
of starfishes. The reproductive cycle was studied by organ indices and histological examination of gonads. The highest gonad
index (GI) values were found in the summer (February–March), followed by a drop in GI values, indicating spawning. Histological
analyses indicated that gametogenesis takes place from June to January, sexual maturity occurred during the summer and spawning
occurred in April. The GI and pyloric caeca index (PCI) tended to have an inverse relationship, but this was only statistically
significant in males. However, biochemical analyses of the starfish indicated no transfer of nutrients between pyloric caeca
and gonads in either sex. In fact, simultaneous accumulation of carbohydrates was found in the pyloric caeca and gonads of
both sexes. 相似文献
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A dark and complex salified organic polymeric mixture, named lignimerin, was for the first time recovered from Kraft cellulose mill wastewater (KCMW) and characterized by chemical, spectroscopic and relative molecular weight (RMW) analysis. Lignimerin proved to be composed of polyphenols (57.00%), carbohydrates (22.26%) and proteins (7.42%). It also contained metals (6.93%), mainly Ca and, to much lower extent, Mg, Na, Al, Fe, K, Mn, Zn and Cu, bound to the carboxylate and phenate groups. The distribution of lignimerin RMW was assessed to be approximately between 1000 and 8600Da, as well as to consist of lignin and tannin, protein and polysaccharide moieties, strongly aggregated each other. H-lignimerin, its acid derivative, revealed a chemical composition and a RMW distribution very close to that of lignimerin, but a marked metal cations decreasing (1.60%) with respect to lignimerin (6.93%). The humic acid-like nature of both the polymers was assessed. Their potential use as bio-adsorbents of heavy metals is briefly discussed. 相似文献
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