Optical and thermal characteristics of carbonaceous aerosols measured at an urban site in Gwangju,Korea, in the winter of 2011

Carbonaceous components (organic carbon [OC] and elemental carbon [EC]) and optical properties (light absorption and scattering) of fine particulate matter (aerodynamic diameter <2.5 μm; PM_2.5) were simultaneously measured at an urban site in Gwangju, Korea, during the winter of 2011. OC was further classified into OC1, OC2, OC3, and OC4, based on a temperature protocol using a Sunset OC/EC analyzer. The average OC and EC concentrations were 5.0 ± 2.5 and 1.7 ± 0.9 μg C m^?3, respectively. The average single-scattering albedo (SSA) at a wavelength of 550 nm was 0.58 ± 0.11, suggesting that the aerosols observed in the winter of 2011 had a local warming effect in this area. During the whole sampling period, “stagnant PM” and “long-range transport PM” events were identified. The light absorption coefficient (b_abs) was higher during the stagnant PM event than during the long-range transport PM event due to the existence of abundant light-absorbing OC during the stagnant PM event. In particular, the OC2 and OC3 concentrations were higher during the stagnant PM event than those during the long-range transport event, suggesting that OC2 and OC3 might be more related to the light-absorbing OC. The light scattering coefficient (b_scat) was similar between the events. On average, the mass absorption efficiency attributed to EC (σ_EC) was 9.6 m² g^?1, whereas the efficiency attributed to OC (σ_OC) was 1.8 m² g^?1 at λ = 550 nm. Furthermore, the σ_EC is comparable among the PM event days, but the σ_OC for the stagnant PM event was significantly higher than that for the long-range transport PM event (1.7 vs. 0.5).

Implications: Optical and thermal properties of carbonaceous aerosol were measured at Gwangju, and carbonaceous aerosol concentration and optical property varied between “stagnant PM” and “long-range transport PM” events. More abundant light absorbing OC was observed during the stagnant PM event.     

Development of competitive immunoassays to hydroxyl containing fungicide metabolites

This paper describes the isolation of monoclonal antibodies and the development of competitive immunoassays to pesticide metabolites of the fungicides imazalil, carbendazim and thiabendazole. The metabolite specific hydroxyl residues were used as the reactive group with which to link the metabolite to the carrier proteins Keyhole Limpet Haemocyanin (KLH) and Bovine Serum Albumin (BSA). In each case immune responses in mice were raised and monoclonal antibodies were produced. Antibodies were developed into competitive ELISAs to the appropriate metabolite. The antibody raised to a metabolite of imazalil was optimised into a competitive ELISA format which had an assay IC50 of 7.5 μg/L and a limit of detection (LOD) of 1.1 μg/L. A single antibody isolated against the metabolite of carbendazim had assay IC50s of 3.2 and 2.7 μg/L for the metabolites of carbendazim and thiabendazole respectively with an LOD of 0.38 μg/L for both. These sensitive immunoassays may have application in the monitoring of human exposure to these fungicide residues either by occupational or non-occupational routes.     

Biodegradation and phytotoxicity assessment of phenanthrene by biosurfactant-producing Bacillus pumilus 1529 bacteria

ABSTRACT

Phenanthrene is a toxic and mutagenic pollutant that can cause severe environmental and human health issues. The bioremediation of these polyaromatic hydrocarbons (PAHs) is possible with a biosurfactant by enhancing hydrophobicity. In this study, the production of a biosurfactant by Bacillus pumilus 1529 and its effects on the phenanthrene biodegradation pathway were examined. Biosurfactant production was determined using hemolytic activity, emulsification index, and surface tension. For phenanthrene metabolite detection, samples at 0, 7, 14, and 21 incubation days were analysed by gas chromatography-mass (GC-mass) spectrometry. The results showed that Bacillus pumilus 1529 can reduce surface tension to 22.83?±?1.1?mN?m^?1. Furthermore, the GC-mass spectrometry analysis showed that 1-hydroxy-2-naphthoic acid, benzaldehyde, o-phthalic acid, and phenylacetic acid were notable phenanthrene metabolites produced during phenanthrene biodegradation. Biodegraded phenanthrene and its metabolites have a less toxic effect on the germination of safflower seeds than non-biodegraded phenanthrene. The IC50 of phenanthrene on seed germination after biodegradation was increased to approximately 113?mg?L^?1. In general, biodegradation aided by biosurfactant producing bacteria contributed to turning the toxic phenanthrene into less harmful metabolites with lower phytotoxicity effects, indicating that its application in the bioremediation of PAHs is promising.     

Assessing the Impact of Areal Precipitation Input on Streamflow Simulations Using the SWAT Model1

Masih Ilyas, Shreedhar Maskey, Stefan Uhlenbrook, and Vladimir Smakhtin, 2011. Assessing the Impact of Areal Precipitation Input on Streamflow Simulations Using the SWAT Model. Journal of the American Water Resources Association (JAWRA) 47(1):179‐195. DOI: 10.1111/j.1752‐1688.2010.00502.x Abstract: Reduction of input uncertainty is a challenge in hydrological modeling. The widely used model Soil Water Assessment Tool (SWAT) uses the data of a precipitation gauge nearest to the centroid of each subcatchment as an input for that subcatchment. This may not represent overall catchment precipitation conditions well. This paper suggests an alternative – using areal precipitation obtained through interpolation. The effectiveness of this alternative is evaluated by comparing its simulations with those based on the standard SWAT precipitation input procedure. The model is applied to mountainous semiarid catchments in the Karkheh River basin, Iran. The model performance is evaluated at daily, monthly, and annual scales by using a number of performance indicators at 15 streamflow gauging stations each draining an area in the range of 590‐42,620 km². The comparison suggests that the use of areal precipitation improves model performance particularly in small subcatchments in the range of 600‐1,600 km². The modified areal precipitation input results in increased reliability of simulated streamflows in the areas of low rain gauge density. Both precipitation input methods result in reasonably good simulations for larger catchments (over 5,000 km²). The use of areal precipitation input improves the accuracy of simulated streamflows with spatial resolution and density of rain gauges having significant impact on results.     

Development of competitive immunoassays to hydroxyl containing fungicide metabolites

This paper describes the isolation of monoclonal antibodies and the development of competitive immunoassays to pesticide metabolites of the fungicides imazalil, carbendazim and thiabendazole. The metabolite specific hydroxyl residues were used as the reactive group with which to link the metabolite to the carrier proteins Keyhole Limpet Haemocyanin (KLH) and Bovine Serum Albumin (BSA). In each case immune responses in mice were raised and monoclonal antibodies were produced. Antibodies were developed into competitive ELISAs to the appropriate metabolite. The antibody raised to a metabolite of imazalil was optimised into a competitive ELISA format which had an assay IC50 of 7.5 μg/L and a limit of detection (LOD) of 1.1 μg/L. A single antibody isolated against the metabolite of carbendazim had assay IC50s of 3.2 and 2.7 μg/L for the metabolites of carbendazim and thiabendazole respectively with an LOD of 0.38 μg/L for both. These sensitive immunoassays may have application in the monitoring of human exposure to these fungicide residues either by occupational or non-occupational routes.     

Climate trends and impacts on crop production in the Koshi River basin of Nepal

Understanding crop responses to climate is essential to cope with anticipated changes in temperature and precipitation. We investigated the climate–crop yield relationship and the impact of historical climate on yields of rice, maize and wheat in the Koshi basin of Nepal. The results show significant impact of growing season temperature and precipitation on crop production in the region. Rice, maize and wheat cultivated at altitudes below 1,100, 1,350 and 1,700 m amsl (above mean sea level), respectively, suffer from stress due to higher temperatures particularly during flowering and yield formation stages. Responses of crop yields to a unitary increment in growing season mean temperature vary from ?6 to 16 %, ?4 to 11 % and ?12 to 3 % for rice, maize and wheat, respectively, depending on the location and elevation in the basin. In most parts of the basin, we observe warming trends in growing season mean temperatures of rice, maize and wheat over the last few decades with clear evidence of negative impacts on yields. However, at some high-elevation areas, positive impacts of warming are also observed on rice and maize yields. If the observed trends in temperature continue in future, the impact is likely to be mostly negative on crop production in the basin. However, crop production may gain from the warming at relatively higher altitudes provided other conditions, e.g., water availability, soil fertility, are favorable.     

Source identification of particulate matter collected at underground subway stations in Seoul,Korea using quantitative single-particle analysis

Subway particle samples collected at four underground subway stations in Seoul, Korea were characterized by a single-particle analytical technique, low-Z particle electron probe X-ray microanalysis. To clearly identify indoor sources of subway particles, four sets of samples collected in tunnels, at platforms, near ticket offices, and outdoors were investigated. For the samples collected in tunnels, Fe-containing particles predominate, with relative abundances of 75–91% for the four stations. The amounts of Fe-containing particles decrease as the distance of sampling locations from the tunnel increases. In addition, samples collected at the platform in subway stations with platform screen doors (PSDs) that limit air-mixing between the platform and the tunnel showed marked decreases in relative abundances of Fe-containing particles, clearly indicating that Fe-containing subway particles are generated in the tunnel. PM₁₀ mass concentration levels are the highest in the tunnels, becoming lower as the distance of sampling locations from the tunnel increases. The extent of the decrease in PM₁₀ in stations with PSDs is also larger than that in stations without PSDs. The results clearly indicate that Fe-containing particles originating in tunnels predominate in the indoor microenvironment of subway stations, resulting in high indoor PM₁₀ levels, and that PSDs play a significant role in reducing Fe-containing particles at platforms and near ticket offices.     

Nondestructive characterization of municipal-solid-waste-contaminated surface soil by energy-dispersive X-ray fluorescence and low-Z (atomic number) particle electron probe X-ray microanalysis

The long-term environmental impact of municipal solid waste (MSW) landfilling is still under investigation due to the lack of detailed characterization studies. A MSW landfill site, popularly known as Dhapa, in the eastern fringe of the metropolis of Kolkata, India, is the subject of present study. A vast area of Dhapa, adjoining the current core MSW dump site and evolving from the raw MSW dumping in the past, is presently used for the cultivation of vegetables. The inorganic chemical characteristics of the MSW-contaminated Dhapa surface soil (covering a 2-km stretch of the area) along with a natural composite (geogenic) soil sample (from a small countryside farm), for comparison, were investigated using two complementary nondestructive analytical techniques, energy-dispersive X-ray fluorescence (EDXRF) for bulk analysis and low-Z (atomic number) particle electron probe X-ray microanalysis (low-Z particle EPMA) for single-particle analysis. The bulk concentrations of K, Rb, and Zr remain almost unchanged in all the soil samples. The Dhapa soil is found to be polluted with heavy metals such as Cu, Zn, and Pb (highly elevated) and Ti, Cr, Mn, Fe, Ni, and Sr (moderately elevated), compared to the natural countryside soil. These high bulk concentration levels of heavy metals were compared with the Ecological Soil Screening Levels for these elements (U.S. Environment Protection Agency) to assess the potential risk on the immediate biotic environment. Low-Z particle EPMA results showed that the aluminosilicate-containing particles were the most abundant, followed by SiO2, CaCO3-containing, and carbonaceous particles in the Dhapa samples, whereas in the countryside sample only aluminosilicate-containing and SiO2 particles were observed. The mineral particles encountered in the countryside sample are solely of geogenic origin, whereas those from the Dhapa samples seem to have evolved from a mixture of raw dumped MSW, urban dust, and other contributing factors such as wind, precipitation, weather patterns, farming, and water logging, resulting in their diverse chemical compositions and the abundant observation of carbonaceous species. Particles containing C and P were more abundant in the Dhapa samples than in the countryside soil sample, suggesting that MSW-contaminated soils are more fertile. However, the levels of particles containing potentially toxic heavy metals such as Cr, Mn, Ni, Cu, Zn, and/or Pb in the Dhapa samples were significant, corroborated by their high bulk concentration levels (EDXRF), causing deep concern for the immediate environment and contamination of the food chain through food crops.