ISOLATION AND DETECTION OF GIARDIA CYSTS FROM WATER USING DIRECT IMMUNOFLUORESCENCE1

ABSTRACT: A water-sampling apparatus used for the isolation and detection of Giardia cysts in water has been designed and tested. The sampling apparatus uses one of a variety of pumps or waterline pressure to move water through a filter. Two of the optional pumps are lightweight enough to make the apparatus portable and thus suitable for sampling in remote areas. This technique of sample processing produces good cyst recovery in much less time than is required with previously established methods. Giardia cysts are identified using direct immunofluorescence.     

Pleistocene diversification of the Pomacentrus coelestis species complex (Pisces: Pomacentridae): historical biogeography and species boundaries

Pleistocene eustatic changes in sea level are often invoked to explain genetic divergence among marine organisms. However, molecular phylogenies have revealed relatively few examples of speciation events dating to the Pleistocene. We present a species-level hypothesis of the timing of evolution for the Pomacentrus coelestis species complex (Pomacentridae), based on the nuclear S7 intron and the mitochondrial Cytb gene, and reconstruct ancestral range distributions across the timetree. Ancestral range reconstruction suggests the complex originated in the Coral Triangle and East Indian Ocean, with subsequent range expansion outward from this region. We suggest that land barriers to dispersal (e.g., Indo-Pacific barrier) may be responsible for the divergence between Indian (P. alleni, P. similis, P. caeruleopunctatus, and P. caeruleus) and Pacific (P. micronesicus, P. auriventris, and P. coelestis) species groups, and subsequent isolation by Pleistocene sea-level fluctuations in certain areas of the Coral Triangle (glacial refugia) may play an important role in the diversification of this species complex. Additionally, our analyses show cryptic lineages within P. micronesicus and highlight the need for comprehensive sampling within and among species to reveal recent speciation events.     

Enhanced anaerobic bioremediation in a DNAPL residual source zone: Test Area North case study

An Interstate Technology and Regulatory Council (ITRC) forum was recently held that focused on case studies in which bioremediation of dense nonaqueous‐phase liquids (DNAPLs) was performed. This first case study, the Test Area North (TAN) site of the Idaho National Engineering and Environmental Laboratory, involves a trichloroethene (TCE) residual source area in a deep, fractured basalt aquifer that has been undergoing enhanced bioremediation since January 1999. Complete dechlorination from TCE to ethene was documented within nine months of operation, and sodium lactate injections were shown to enhance TCE mass transfer from the residual source. Since that time, optimization of injection strategies has maintained efficient dechlorination while demonstrating accelerated cleanup at a lower cost by changing to a whey powder amendment that solubilizes DNAPL. © 2006 Wiley Periodicals, Inc.     

Activity-dependent labeling of oxygenase enzymes in a trichloroethene-contaminated groundwater site

A variety of naturally occurring bacteria produce enzymes that cometabolically degrade trichloroethene (TCE), including organisms with aerobic oxygenases. Groundwater contaminated with TCE was collected from the aerobic region of the Test Area North site of the Idaho National Laboratory. Samples were evaluated with enzyme activity probes, and resulted in measurable detection of toluene oxygenase activity (6-79% of the total microbial cells). Wells from both inside and outside contaminated plume showed activity. Toluene oxygenase-specific PCR primers determined that toluene-degrading genes were present in all groundwater samples evaluated. In addition, bacterial isolates were obtained and possessed toluene oxygenase enzymes, demonstrated activity, and were dominated by the phylotype Pseudomonas. This study demonstrated, through the use of enzymatic probes and oxygenase gene identification, that indigenous microorganisms at a contaminated site were cometabolically active. Documentation such as this can be used to substantiate observations of natural attenuation of TCE-contaminated groundwater plumes.     

Employee behaviors before and after stress management

A quasi-experiment was used to compare employee behaviors before and after stress management training. Organizational records on employee absenteeism, performance ratings, equipment accidents, and work injuries were obtained for highway maintenance workers who received biofeedback (BIO=21) or muscle relaxation (MR=16) training. Similar data were gathered for a comparison group of employees who did not volunteer for training (n = 80). The pre-training period (time 1) consisted of 2 1/2 years. The post-training period was divided into the year immediately after training (time 2) and the following 1/2 year (time 3). Multiple regression analyses indicated that the MR (but not BIO) group variable explained unique variance in time 2 absenteeism (p < 0.05) but not in performance ratings, equipment accidents, nor work injuries (p > 0.05). Neither group entered predictive models for any measure at time 3 (p > 0.05). The results provide limited support for relaxation training offered as a prevention activity in work settings.     

A Study of Wood Stove Particulate Emissions

Particulate emission factors for two wood stove models have been determined for two types of fuel and a range of operating conditions. The emission factors range from 1 g/kg (fuel) to 24 g/kg. A model is presented which represents the emission factor as a simple function of the ratio of fuel load to combustion rate, or the length of time between refueling. This model is felt to be appropriate for evaluating the impact of wood-based residential space heating on ambient air concentrations of particulate matter If certain assumptions can be made about stove operating conditions. An application of the emission factor model to a typical community suggests that the contribution of wood stoves to ambient particulate levels might reach 100 μg/m³ if the entire heating load were carried by wood.

Preliminary analyses of the particulate matter Indicate that benzene extractables range from 42% of the total particulate mass at short refuel times to 67% at longer refuel times. About 45% of the mass of benzene extractables appeared in the neutral fraction of acid base extractions. Polycyclic aromatic hydrocarbons are expected to be included in this neutral fraction.     

Sexual selection in the northern pintail (Anas acuta): the importance of female choice versus male-male competition in the evolution of sexually-selected traits

We experimentally studied the relative importance of plumage, dominance status, and courtship behavior in determining male pairing success in the northern pintail Anas acuta and assessed whether these traits function in female choice, male-male competition or both. In an experiment (experiment IA) that eliminated the confounding effects of male-male competition and social courtship, females chose males with pure white breasts and colorful scapular feathers. When the same group of birds were free to interact (experiment 1B), male behavior was more important: females chose males that courted them intensely and were attentive to them, although preferred males again had whiter breasts and more colorful scapulars. In a second experiment (experiment 2), testing the effect of age on pairing success, females showed a significant preference for 2-year-old males over yearlings: 2-year-old males courted more and were more attentive to the female than yearlings; they were also more colorful than yearlings in a number of plumage measurements. Although males (in both experiments 1B and 2) were aggressive to one another while courting the female and dominant males were sometimes able to exclude subordinates from social courtship, contrary to expectation, we found no relationship between initial dominance rank and pairing success or dominance rank and age. In addition, dominance was not correlated with any of the morphological traits measured. Once chosen, however, subordinate males typically initiated fights with the higher-ranked male(s) and quickly achieved dominance. These results suggest that (1) females choose males based on a suite of morphological and behavioral characteristics, (2) male dominance relationships do not constrain active female choice, (3) a male's position in a dominance hierarchy is largely a result rather than a cause of female choice, and (4) female choice plays a more significant role than male-male competition in the evolution of several secondary sexual traits in male northern pintails.     

The role of figural organization in city imageability: An information processing analysis

The purpose of this study was to apply experimental methods and theoretical formulations from the information processing metaphor in experimental psychology to explore some of the underlying theoretical premises in Lynch's conception of imageability. Specifically, the study attempted to separate and assess the effects of the salience and organization of schematic maps' path networks on the imageability of the maps. Findings support Lynch's contention that organization is critical to a highly imageable urban environment. At the same time, our results go beyond Lynch by suggesting that identifiability or visual emphasis alone, when unorganized, may detract from rather than aid city legibility. Results are interpreted in terms of limitations in cognitive processing capacity and the cognitive resources required by the map learning task.