Trophoblast-like cells sorted from peripheral maternal blood using flow cytometry: A multiparametric study involving transmission electron microscopy and fetal dna amplification

Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast-like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y-specific sequences from mothers carrying male fetuses. At the ultra-structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter-villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y-specific sequences in two out of three samples of sorted TLCs. These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non-invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities.     

Successful DNA-based prenatal exclusion of juvenile neuronal ceroid lipofuscinosis

A family with two siblings, 10 and 8 years old, both with clinical and ultrastructural evidence of juvenile neuronal ceroid lipofuscinosis is described. The family was found to be informative for the restriction fragment length polymorphisms (RFLPs) detected by the probes pCJ52–95Ml (locus D16S148) and pCJ52-94Tl (locus D16S159) flanking the juvenile neuronal ceroid lipofuscinosis locus, CLN3. The parents were both heterozygous using these probes, while their two children with juvenile neuronal ceroid lipofuscinosis were both homozygous. Chorionic villi analysis showed that the fetus was heterozygous and had inherited the one allele of the mother which was not found in the two siblings. This suggested that the fetus had derived one healthy allele from the mother, the risk for a double crossing-over being less than 1 per cent. Electron microscopy showed no fingerprint inclusions in chorionic villi. The child was investigated at 6 months of age and found to be healthy, as new fingerprint inclusions were found at electron microscopy and no vacuolated lymphocytes were found in the blood smear. Due to the risk of heterogeneity, both DNA-based analysis and electron microscopy on chorionic villi are recommended for prenatal examination for juvenile neuronal ceroid lipofuscinosis.     

电子废弃物的特点

电子废弃物资源化研究是一项新型而紧迫的课题，它具有数量多、危害大、潜在价值高、回收利用困难的特点，必须对电子废弃物予以高度关注。     

电子束法烟气净化工艺主要因素分析

电子束法烟气净化技术是近年来发展较快的一项烟气净化技术。在清华大学核能技术设计研究院的电子束法烟气净化装置的实验基础上，通过对大量实验数据的分析，找出工艺中各种因素对脱硫率与脱硝率的影响。辐照剂量[D]、烟气温度[T]、含湿量[H]、注氨当量[α]、SO2的初始浓度[CSO2]和NOx的初始浓度[CNOx]是影响烟气脱硫脱硝的基本因素。文章采用相关分析、回归分析的方法，找出主要的因素，建立回归经验方程，以优化系统工艺，简化系统结构，预测实验结果。     

烧结烟气应用电子束脱硫脱硝技术之利弊

介绍了电子束辐照烟气脱硫技术的原理、工艺流程、主要设备及其作用;中国工程物理研究院1.2万m^3/h电子束脱硫脱硝试验装置及试验结论;成都热电厂30万m^3/h脱硫示范装置的设计参数、运行情况;综合分析了烧结烟气脱硫应用电子束辐照烟气脱硫脱硝技术之利弊,认为烧结烟气脱硫应用电子束辐照烟气脱硫技术不失为一个可供选择且可行的技术.     

混凝土简支梁设计基准期内的可靠度

根据统计资料对不同类型建筑在不同防火措施下发生火灾的概率研究,得出了各类建筑达到轰燃的概率.基于蒙特卡罗随机有限元方法,引入材料高温本构关系、截面尺寸和计算模型系数等的变异性,按照ISO标准升温曲线升温,给出了单构件轰燃下的失效概率计算方法.最后,将设计基准期内建筑物达到轰燃的概率与单构件轰燃下的失效概率组合,给出了设计基准期内建筑构件在火灾下的失效概率公式.     

Proposing of new building scheme and composite towards global warming mitigation for Malaysia

The building sector has been regarded as a potential sector where there is large capacity to reduce the climate change effect. This study has proposed solutions to mitigate environmental impacts and achieve low CO₂ emission from residential sector. Therefore, full life cycle assessment (LCA) has been run to assess the CO₂ emission and its effect on the atmosphere and climate change. Based on the result, timber scheme is the best choice due to releasing less CO₂ emissions to the atmosphere. However, house builders in Malaysia have almost completely neglected timber as a building material, with timber use as building components reduced to 5%. In this study, LCA Software was used to assess CO₂ emissions from different wall construction. The alternative building scheme has been made by reinforce steel stud, wooden beam and timber wall (S8) to improve the scheme deficiency while releasing less CO₂ emissions compared to other schemes. Therefore, S8 has a decreased CO₂ effect by 85% less than precast concrete frame and 90% less than brick over their lifetime. (S8) increased the load bearing compared to conventional timber beam. Thus, new scheme S8 could be replaced by current scheme and promote more adjustable scheme for Malaysian housing.     

Sulfur cycle as an electron mediator between carbon and nitrate in a constructed wetland microcosm

• Fe(III) accepted the most electrons from organics, followed by NO₃^‒, SO₄^2‒, and O₂. • The electrons accepted by SO₄^2‒ could be stored in the solid AVS, FeS₂-S, and S⁰. • The autotrophic denitrification driven by solid S had two-phase characteristics. • A conceptual model involving electron acceptance, storage, and donation was built. • S cycle transferred electrons between organics and NO₃^‒ with an efficiency of 15%. A constructed wetland microcosm was employed to investigate the sulfur cycle-mediated electron transfer between carbon and nitrate. Sulfate accepted electrons from organics at the average rate of 0.84 mol/(m³·d) through sulfate reduction, which accounted for 20.0% of the electron input rate. The remainder of the electrons derived from organics were accepted by dissolved oxygen (2.6%), nitrate (26.8%), and iron(III) (39.9%). The sulfide produced from sulfate reduction was transformed into acid-volatile sulfide, pyrite, and elemental sulfur, which were deposited in the substratum, storing electrons in the microcosm at the average rate of 0.52 mol/(m³·d). In the presence of nitrate, the acid-volatile and elemental sulfur were oxidized to sulfate, donating electrons at the average rate of 0.14 mol/(m³·d) and driving autotrophic denitrification at the average rate of 0.30 g N/(m³·d). The overall electron transfer efficiency of the sulfur cycle for autotrophic denitrification was 15.3%. A mass balance assessment indicated that approximately 50% of the input sulfur was discharged from the microcosm, and the remainder was removed through deposition (49%) and plant uptake (1%). Dominant sulfate-reducing (i.e., Desulfovirga, Desulforhopalus, Desulfatitalea, and Desulfatirhabdium) and sulfur-oxidizing bacteria (i.e., Thiohalobacter, Thiobacillus, Sulfuritalea, and Sulfurisoma), which jointly fulfilled a sustainable sulfur cycle, were identified. These results improved understanding of electron transfers among carbon, nitrogen, and sulfur cycles in constructed wetlands, and are of engineering significance.     

Effect of acid solutions on plants studied by the optical beam deflection method

The optical beam deflection method was applied to study the effects of acid solution on both a terrestial and aquatic plants Egeria and Cerastium, which are common aquatic plant and terrestial weed respectively. A probe beam from a He-Ne laser was passed through a vicinity of a leaf of the plants, which were put in culture dishes filled with acid solutions. Deflection signals of the probe beam were monitored and compared for acid solutions with different pH values. The results of Egria showed that the deflection signals changed dramatically when pH values of acid solutions were 2.0 and 3.0, while little at pH of 4.0 and 5.0. For Cerastium when pH were below 3.0, deflection signals changed greatly with time at the begining. After a certain period of time, deflection signals changed little with time. When pH value was above 4.0, deflection signals of Cerastium were still changing with time even after 20 hours. The results suggested that the damage threshold of pH was between 3.0 and 4.0 for both the land and aquatic plants.