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1.
Fragile X syndrome is the most common cause of familial mental retardation. The most common mutation is expansion of a triplet (CGG)n repeat in the 5′ untranslated region of the FMR1 gene on Xq27.3. The expansion is refractory to PCR due to preferential amplification of the smaller allele in heterozygous cells and the high GC content of the repeat and surrounding sequences. Direct detection of the normal parental alleles in preimplantation embryos has been used for preimplantation genetic diagnosis (PGD) of this disorder. However, this approach is only suitable for approximately 63% of couples due to the heterozygosity of the repeat in the normal population. As an alternative we investigated the use of polymorphic markers flanking the mutation to track the normal and premutation carrying maternal chromosomes in preimplantation embryos. Using a panel of 11 polymorphisms, six (CA)n repeats and five single nucleotide polymorphisms, diagnosis was developed for 90% of referred couples. Multiplex amplification of informative markers was tested in 300 single buccal cells from interested couples with efficiency and allele drop out (ADO) rates ranging from 69% to 96% and 6% to 18%, respectively. Use of this approach is accurate and applicable to a larger number of patients at risk of transmitting fragile X to their offspring. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
2.
长江流域的昆虫病毒资源及其开发利用 总被引:1,自引:0,他引:1
在长江流域已发现约130种昆虫病毒,其中多数属杆状病毒科。昆虫病毒作为杀虫剂在本地区广泛应用,防治棉花、森林、茶树、蔬菜和牧草上的害虫,克服了化学农药的一些缺点,取得了良好的生态效益和经济效益。近年来还进行了杆状病毒表达载体的研究,该系统的研制已用于高水平表达具有生物活性的外源基因产物,获得医药产品,或者组建更有效的基因工程病毒杀虫剂。 相似文献
3.
Alan R. Thornhill John A. McGrath Robin A. J. Eady Peter R. Braude Alan H. Handyside 《黑龙江环境通报》2001,21(6):490-497
Single cell polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD) requires high efficiency and accuracy. Allele dropout (ADO), the random amplification failure of one of the two parental alleles, remains the most significant problem in PCR-based PGD testing since it can result in serious misdiagnosis for compound heterozygous or autosomal dominant conditions. A number of different strategies (including the use of lysis buffers to break down the cell and make the DNA accessible) have been employed to combat ADO with varying degrees of success, yet there is still no consensus among PGD centres over which lysis buffer should be used (ESHRE PGD Consortium, 1999 ). To address this issue, PCR amplification of three genes (CFTR, LAMA3 and PKP1) at different chromosomal loci was investigated. Single lymphocytes from individuals heterozygous for mutations within each of the three genes were collected and lysed in either alkaline lysis buffer (ALB) or proteinase K/SDS lysis buffer (PK). PCR amplification efficiencies were comparable between alkaline lysis and proteinase K lysis for PCR products spanning each of the three mutated loci (ΔF508 in CFTR 90% vs 88%; R650X in LAMA3 82% vs 78%; and Y71X in PKP1 91% vs 87%). While there was no appreciable difference between ADO rates between the two lysis buffers for the LAMA3 PCR product (25% vs 26%), there were significant differences in ADO rates between ALB and PK for the CFTR PCR product (0% vs 23%) and the PKP1 PCR product (8% vs 56%). Based on these results, we are currently using ALB in preference to PK/SDS buffer for the lysis of cells in clinical PGD. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
4.
Cardiac anomalies may occur in isolation or can be part of a genetic syndrome. In this article, we describe some of the genetic syndromes commonly associated with cardiac anomalies where there are other sonographic features that may aid accurate prenatal diagnosis. Copyright © 2004 John Wiley & Sons, Ltd. 相似文献
5.
Henrietta Nittby Bengt Widegren Morten Krogh Gustav Grafström Henrik Berlin Gustav Rehn Jacob L. Eberhardt Lars Malmgren Bertil R. R. Persson Leif G. Salford 《The Environmentalist》2008,28(4):458-465
We have earlier shown that radio frequency electromagnetic fields can cause significant leakage of albumin through the blood–brain
barrier of exposed rats as compared to non-exposed rats, and also significant neuronal damage in rat brains several weeks
after a 2 h exposure to a mobile phone, at 915 MHz with a global system for mobile communications (GSM) frequency modulation,
at whole-body specific absorption rate values (SAR) of 200, 20, 2, and 0.2 mW/kg. We have now studied whether 6 h of exposure
to the radiation from a GSM mobile test phone at 1,800 MHz (at a whole-body SAR-value of 13 mW/kg, corresponding to a brain
SAR-value of 30 mW/kg) has an effect upon the gene expression pattern in rat brain cortex and hippocampus—areas where we have
observed albumin leakage from capillaries into neurons and neuronal damage. Microarray analysis of 31,099 rat genes, including
splicing variants, was performed in cortex and hippocampus of 8 Fischer 344 rats, 4 animals exposed to global system for mobile
communications electromagnetic fields for 6 h in an anechoic chamber, one rat at a time, and 4 controls kept as long in the
same anechoic chamber without exposure, also in this case one rat at a time. Gene ontology analysis (using the gene ontology
categories biological processes, molecular functions, and cell components) of the differentially expressed genes of the exposed
animals versus the control group revealed the following highly significant altered gene categories in both cortex and hippocampus:
extracellular region, signal transducer activity, intrinsic to membrane, and integral to membrane. The fact that most of these
categories are connected with membrane functions may have a relation to our earlier observation of albumin transport through
brain capillaries. 相似文献
6.
Wayfinding with a GPS-based mobile navigation system: A comparison with maps and direct experience 总被引:2,自引:0,他引:2
Toru Ishikawa Hiromichi Fujiwara Osamu Imai Atsuyuki Okabe 《Journal of environmental psychology》2008
This study examined the effectiveness of a Global Positioning System (GPS)-based mobile navigation system in comparison to paper maps and direct experience of routes, by focusing on the user's wayfinding behavior and acquired spatial knowledge. Based on information received from one of these three media, participants walked six routes finding the way to goals. Results showed that GPS users traveled longer distances and made more stops during the walk than map users and direct-experience participants. Also, GPS users traveled more slowly, made larger direction errors, drew sketch maps with poorer topological accuracy, and rated wayfinding tasks as more difficult than direct-experience participants. Characteristics of navigation with these three learning media and possible reasons for the ineffectiveness of the GPS-based navigation system are discussed. 相似文献
7.
水安全危机是人类进入新世纪以来在生存及发展方面所面临的最严重挑战之一。研究科学合理的水安全战略成为区域可持续发展的重大课题。针对区域水战略问题涉及众多因素且各因素之间动态关联的特点,提出了基于水战略方案优选的兼容度极大化动态组合评价模型(CMM—DCEM),并将其成功地应用于我国广东省北江下游及其三角洲地区水安全战略方案优选评价。评价结果及模型基于不确定性的敏感性分析结果证明:CMM—DCEM实现了主、客观赋权方法以及单一评价模型的融合,使用实码加速遗传算法求解目标函数,克服了传统的组合评价方法计算繁琐的不足,评价过程更加科学合理。 相似文献
8.
9.
针对河流水污染应急响应过程中污染源排放历史迟知、未知的问题,结合多种群遗传算法和自适应遗传算法,利用一维河流水质模型和水质监测数据,研究建立了基于改进遗传算法的河流水污染定量源反演方法,实现了对河流污染源排放历史的识别与重构.将该方法应用于美国特拉基河流的3个不同流量下的示踪剂实验中,对示踪剂排放历史进行定量源反演分析.结果表明:IGA算法对高、中、低不同流量下的3次示踪剂实验均可以很好的重构和识别示踪剂排放历史,对于实际河流水污染源反演分析的误差均在可接受范围内.IGA算法在河流水污染源反演分析中具有一定的可靠性和稳定性,可为河流水污染精准溯源与治理提供科学的技术支撑. 相似文献
10.
为研究机动车道路行驶过程中轮胎磨损排放的颗粒物理化特性,利用轮胎轮廓仿真磨耗仪,对国内主流17种轮胎胎面进行仿真磨耗实验,获得颗粒物样品,提取并检测其中18种元素和20种多环芳烃(PAHs)的含量.结果显示,元素和PAHs含量因轮胎品牌和速度等级的不同而差异显著.18种元素平均含量为(99.04±68.43)mg/g,占样品总重的9.90%,其中Si(88.97±67.85)mg/g、Zn(6.77±1.64)mg/g和Na(1.05±0.75)mg/g的平均含量均超过1mg/g,Cd的含量最低,为(0.43±0.31)μg/g.20种PAHs含量之和(∑20PAHs)在12.13~433.64 μg/g,平均为(94.13±110.18)μg/g,PY的平均含量最高(30.98±31.27)μg/g,其次是CHR、BaP、FA、PHE和BghiP,平均含量最低的是AC(0.58±0.2)μg/g;从环数看,以4环PAHs为主(占∑20PAHs的45.03%~67.93%),其次为3环(平均含量为15.45%)和5环(平均含量为12.62%).总体来说,国外品牌轮胎样品中元素和PAHs含量略高于国内品牌,而主要PAHs环数略低于国内品牌. 相似文献