基于RF-LSTM的鸡舍恶臭气体预测研究

以鸡舍氨气为研究对象,对鸡舍氨气预测模型进行了研究.首先,利用随机森林算法（RF）对影响鸡舍氨气浓度的环境变量进行重要性排序,选取温度、湿度、光照、气象温度、降雨量作为模型的输入变量;在此基础上,构建了基于长短时记忆神经网络（LSTM）的鸡舍氨气浓度预测模型,并将提出的预测模型应用于江苏省宜兴市某养鸡场的氨气浓度预测中,并与LSTM模型、RF-Elman模型和RF-BP模型进行了对比实验,结果表明,基于RF-LSTM模型的预测效果最好,其平均绝对误差（MAE）、平均绝对百分误差（MAPE）和均方根误差（RMSE）分别为0.9183、4.9637%和1.4262;同时,为了验证该模型的性能,本文还实现了不同时间尺度的鸡舍氨气浓度预测,提前2h、3h、4h、5h氨气预测的平均绝对误差（MAE）分别为1.6218、2.1991、2.8553和3.0677.本文提出的预测模型提高了鸡舍氨气浓度的预测精度,可为减少鸡舍恶臭气体排放提供科学依据.     

我国热带地区胶—茶—鸡农林复合系统物质循环研究

以海南省文昌市典型的胶—茶—鸡农林复合模式为研究对象 ,以单作胶园、胶茶间作园为对照 ,采用定量研究与定性分析相结合的方法 ,研究其物质循环规律。结果表明 ,胶—茶—鸡农林复合系统的氮、磷输入、输出量最大 ,系统外物质投入率比另两系统明显减少 ,系统内养分循环量增加 ,因此 ,该系统具有较合理的物质循环结构。     

硝基芳烃化合物诱发细胞微核的比较研究

利用蚕豆根法细胞和人在淋巴细胞的微核试验对６种硝基芳烃化合物的致突变性进行比较研究，结果表明，邻二硝基苯、间二硝基苯、２，４－二硝基甲苯，２，６－二硝基甲苯，对硝基氯苯和对硝基溴苯均能诱发两咱细胞的微核率增加，除了对硝基溴苯外均具有明显剂量一效应关系。引进标准剂量概念比较５种硝基芳烃人合物的致突变性，结果表明，致突变性强弱依次为２，４－二硝基甲苯〉对硝基氯苯〉间二硝基苯〉２，６－二硝基甲苯〉邻二硝     

Effect of fipronil on biogas production performance during anaerobic digestion of chicken manure and corn straw

Fipronil is a broad-spectrum insecticide that has a good control effect on pests of commercial poultry. Although many studies have reported the environmental fate of fipronil, the influence of residual fipronil in poultry waste on biogas production has not been further explored yet. In this article, an experimental comparative study on anaerobic digestion (AD) of chicken manure (CM) and corn straw (CS) with different fipronil concentrations (FCs) was carried at 8% of total solid (TS) and mid-temperature (35?±?1)°C. The results showed that fipronil had a significant effect on biogas production during AD of CM and CS. When the FC is at a low level (≤10?mg·kg^?1), the biogas production rate is increased and the digestion period was shortened, while higher FC (≥ 20?mg·kg^?1) showed an inhibitory effect. During the monitoring of enzyme activity, low FC showed no significant effect on cellulase and saccharase, but the urease activity increased in the early stage. High FC showed inhibition of activity of cellulase and urease, but the saccharase activity was significantly inhibited until FC reached 40?mg·kg^?1. This study also confirms that the environment in anaerobic digester is favorable for the degradation of fipronil, and its half-life is about 15.83?days.     

Immunotoxicity of bisphenol a to Carassius auratus Lymphocytes and macrophages following in vitro exposure

Bisphenol A （BPA） is the monomer component of polycarbonate plastics and classified as an endocrine disrupting chemical （EDC）. The reproductive toxicity of BPA has been extensively studied in mammals; however, relatively little information is available on the immunotoxic responses of fish to BPA. In this study, we investigated the effects of BPA on the immune functions of lymphocytes and macrophages in Carassius auratus. The effects of BPA were compared with those of two natural steroid hormones, estradiol and hydrocortisone. Proliferation of the two types of cells in response to PHA was measured using colorimetric MTT assay. Macrophage respiratory burst stimulated by Con A was measured using chemiluminescence assay. Results showed that BPA （0.054-5.4 mg/L）, estradiol （0.0002-2.0 mg/L） and hydrocortisone （5-50 mg/L） significantly induced Carassius auratus lymphocyte proliferation while higher doses of hydrocortisone （500-5000 mg/L） appeared to be inhibitory. BPA （0.005-50 mg/L）, estradiol （0.005-800 mg/L） and hydrocortisone （0.005-500 mg/L） markedly enhanced macrophage proliferation, whereas higher doses of BPA （500-1000 mg/L） appeared to inhibit cell proliferation. Furthermore, higher dosage of BPA （50 mg/L） and hydrocortisone （50 and 500 mg/L） suppressed the macrophages respiratory burst while estradiol is stimulative all the doses tested （0.05-500 mg/L）. In conclusion, BPA could have immunotoxicity to Carassius auratus and functional changes of lymphocyte and macrophage in Carassius auratus may be different between low and high dosages.     

Micronucleus frequency in sheep lymphocytes after in vitro exposure to fungicide tolylfluanid

The fungicide tolylfluanid (N - dichlorofluoromethylthio-N′, N - dimethyl - N - p - tolylsulfamide), was investigated by cytokinesis-block micronucleus assay. Tolylfluanid at the lowest concentration (1 × 10^{? 6}mol L^{? 1})did not influence significantly the frequency of micronuclei in sheep lymphocyte cultures in comparison with control (32.33 ± 3.51/1000 binucleated cells versus 30.33 ± 2.82/1000 binucleated cells in dimethylsulfoxide (DMSO) control, P = 0.44). Higher tolylfluanid concentrations (1 × 10^{? 4} and, 1 × 10^{? 5} mol L^{? 1}) resulted in a significant dose-dependent increase in the number of micronuclei in comparison with control (74.00 ± 13.00/1000 binucleated cells and 52.67 ± 10.12/1000 binucleated cells versus 30.33 ± 2. 82/1000 binucleated cells in DMSO control, P = 0.005 and 0.02, respectively, ANOVA followed by Tukey test P < 0.05). Many of the treated cells also possessed multiple micronuclei. Tolylfluanid did not affect the nuclear division index at all treatment concentrations. Our in vitro results thus demonstrate that tolylfluanid had a significant genotoxic effect at only the highest concentration tested.     

Comparative effect of thymol or its glucose conjugate,thymol-β-d-glucopyranoside,on Campylobacter in avian gut contents

Campylobacter jejuni is an important human food-borne pathogen that can contaminate meat and poultry during processing. Consequently, strategies are sought to reduce the carriage of C. jejuni in food animals before they arrive at the abattoir. Thymol is a natural product that reduces survivability of Campylobacter in vitro, but its rapid absorption from the proximal alimentary tract limits its bactericidal efficacy in vivo. Thymol-β-d-glucopyranoside is more resistant to absorption than free thymol, but its administration to chickens has not been reported. In the present studies, 1 mM thymol-β-d-glucopyranoside was shown to exhibit near equal anti-Campylobacter activity as 1 mM thymol when incubated anaerobically in avian crop or cecal contents in vitro, resulting in reductions of 1.10–2.32 log₁₀ colony forming units mL^?1 in C. jejuni concentrations after 24 h incubation. In a follow-up live animal study, oral administration of thymol-β-d-glucopyranoside, but not free thymol, significantly lowered (>10-fold) recovery of Campylobacter from the crop of market-aged broilers when compared to placebo-treated controls (n = 6 broilers/treatment). Neither thymol-β-d-glucopyranoside nor thymol affected recovery of Campylobacter from cecal contents of the treated broilers. These results indicate that rapid absorption or passage of free thymol from the crop precluded its anti-Campylobacter activity at this site and throughout the entire gastrointestinal tract. Conversely, lower recovery of Campylobacter from the crop of birds treated with thymol-β-d-glucopyranoside indicates this conjugate was retained and able to be hydrolyzed to biologically active free thymol at this site as intended, yet was not sufficiently protected to allow passage of efficacious amounts of the intact glycoside to the lower gut. Nevertheless, these results warrant further research to see if higher doses or encapsulation of thymol-β-d-glucopyranoside or similar glycosides may yield an efficacious additive to reduce carriage of Campylobacter as well as other pathogens throughout the avian gut.     

Chicken manure enhanced yield and quality of field-grown kale and collard greens

Organic matter and nutrients in municipal sewage sludge (SS) and chicken manure (CM) could be recycled and used for land farming to enhance fertility and physical properties of soils. Three soil management practices were used at Kentucky State University Research Farm, Franklin County, to study the impact of soil amendments on kale (Brassica oleracea cv. Winterbar) and collard (Brassica oleracea cv. Top Bunch) yields and quality. The three soil management practices were: (i) SS mixed with native soil at 15 t acre^?1, (ii) CM mixed with native soil at 15 t acre^?1, and (iii) no-mulch (NM) native soil for comparison purposes. At harvest, collard and kale green plants were graded according to USDA standards. Plants grown in CM and SS amended soil produced the greatest number of U.S. No. 1 grade of collard and kale greens compared to NM native soil. Across all treatments, concentrations of ascorbic acid and phenols were generally greater in kale than in collards. Overall, CM and SS enhanced total phenols and ascorbic acid contents of kale and collard compared to NM native soil. We investigated the chemical and physical properties of each of the three soil treatments that might explain variability among treatments and the impact of soil amendments on yield, phenols, and ascorbic acid contents of kale and collard green grown under this practice.     

Evaluation of protective effects of sulforaphane on DNA damage caused by exposure to low levels of pesticide mixture using comet assay

The objective of the study was to evaluate the potential risk of DNA damage due to exposure to a mixture of the most widely used pesticides, namely endosulfan, chlorpyriphos and thiram at an environmentally relevant concentration (5 μM each) and the DNA protective capacity of sulforaphane (SFN) (10–30 μg/mL). DNA damage in human lymphocytes was ascertained with Single Cell Gel Electrophoresis (SCGE), also called Comet Assay. For positive control, H₂O₂ at 100 mM was used. The pesticide mixture produced DNA damage at the concentration used in the lymphocytes. SFN was able to offer a statistically significant (P < 0.01), concentration-dependant protection to DNA damage between 10–20 μg/mL in both the pre-incubation and co-incubation strategies. The results indicate that exposure to low levels of these pesticide mixtures can induce DNA damage, and the presence of SFN in diet may reduce the incidence of genetic damage, especially in farm workers. However, it is not clear whether SFN is involved in quenching of the free radicals generated by the pesticide mixture or it is involved in DNA repair mechanism.     

Microwave extraction of incurred salinomycin from chicken tissues

Abstract

A rapid, accurate, environmentally friendly and cost‐effective microwave extraction technique was developed for the extraction of spiked and incurred salmomycin from chicken tissues (kidney, liver, muscle, ovarian yolk and fat). Extraction of salinomycin from various tissues was achieved by irradiating the sample in absolute ethanol and 2‐propanol (15+2) for 9 sec. in a common household microwave oven. The extract was analysed without further cleanup by HPLC on a C₁₈ column (5 μm) and detected at 592 nm via post‐column reaction with 4‐dimethylaminobenzaldehyde (DMABA) in a heated reactor coil at 86° C. Recoveries of salinomycin from spiked tissues at 30 ng/g level ranged between 87 and 100%. The limit of quantitation was found to be 10 ng/g. The developed method was applied for the analysis of incurred tissues and ovarian yolk of laying chickens given sodium salinomycin in feed at different levels for 14 consecutive days followed by withdrawal periods. Residues were detected in all tissues and ovarian yolk at 0 withdrawal time but declined during the withdrawal period. Highest residue were found in fat and ovarian yolk.