尼克酰胺与核黄素提高红串红球菌USTB-03对4,6-二甲基二苯并噻吩脱硫活性研究

主要考察了尼克酰胺和核黄素提高红串红球菌USTB-03对4,6-二甲基二苯并噻吩(4,6-DMDBT)脱硫活性的效应。研究结果表明,在培养基中添加初始浓度为10 mmol/L的尼克酰胺,脱硫比活性由原来302.4 mmol 4,6-DMDBT/(kg细胞干重·h)增加到476.4 mmol 4,6-DMDBT/(kg细胞干重·h),提高了57.5%。添加初始浓度为25μmol/L的核黄素,脱硫比活性增加到了465.8 mmol 4,6-DMDBT/(kg细胞干重·h),提高了54%。无论是采用微量的尼克酰胺或核黄素都可以大幅度提高红串红球菌USTB-03对4,6-DMDBT的脱硫活性,在此研究领域尚未发现有研究报道。尼克酰胺和核黄素分别是NAD和FMN的主要成分,可以促进脱硫关键辅酶FMNH_2的生物合成,因此,大幅度提高了红串红球菌USTB-03对4,6-DMDBT的脱硫活性。

Increasing biodesulfurization activity of 4,6-dimethyldibenzothiophene of <>iRhodococcus erythropolis USTB-03 using nicotinamide and riboflavin

Effects of nicotinamide and riboflavin on the sulfur specific activity ( SA) of 4,6-dimethyl-dibenzothiophene (4, 6-DMDBT) biodesulfurization by Rhodococcus erythropolis USTB-03 were investigated. When R. erythropolis USTB-03 was grown on medium containing nicotinamide of 10 mmol/L, SA was raised 57.5% from 302.4 to 476.4 mmol 4,6-DMDBT/( kg-dry cells · h). When R. erythropolis USTB-03 was grown on medium containing riboflavin of 25 /tmol/L, SA increased to 465. 8 mmol 4,6-DMDBT/( kg-dry cells · h) , which was 54% higher than that of control. Both nicotinamide and riboflavin were found to enhance expression of SA, which was not previously reported. It is suggested that the biological synthesis of FMNH_2, an essential coen-zyme for the activities of biodesulfurization enzyme, might be enhanced by nicotinamide and riboflavin, which was responsible for the increased SA of R. erythropolis USTB-03.