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Alga-lysing bioreactor and the dominant bacteria strain
Authors:PEI Hai-yan  HU Wen-rong  MU Rui-min  LI Xiao-cai
Abstract:Alga-lysing bacteria have been paid much attention to in recent years. In this study, the alga-lysing strain P05 which was isolated from an immobilizing biosystem was immobilized by coke and elastic filler, forming two biological reactors. The removal efficiencies of algae, NH3-N and organic matter using the two reactors were studied. The results showed that strain P05 was an ideal algal-lysing bacteria strain because it was easy to be immobilized by coke and elastic filler which are of cheap, low biodegradability and the simple immobilization procedure. After 7 d filming, the biological film could be formed and the reactors were used to treat the eutrophic water. These two reactors were of stability and high effect with low cost and easy operation. The optimal hydraulic retention time (HRT) of each reactor was 4 h. The algae removal rates were 80.38% and 82.1% (in term of Chl-a) of coke reactor and filler reactor, respectively. And that of NH3-N were 52.3% and 52.7%. The removal rates of CODMn were 39.03% and 39.64%. The strain P05 was identified as Bacillus sp. by PCR amplification of the 16S rRNA gene, BLAST analysis, and comparison with sequences in the GenBank nucleotide database.
Keywords:alga-lysing  algae removal  immobilization  16S rRNA  Bacillus sp  bacteria strain  comparison  sequences  GenBank  nucleotide  database  BLAST  analysis  amplification  rRNA  gene  Bacillus sp  CODMn  term  algae removal  rates  reactor  optimal  hydraulic retention time  stability
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