| PCR-酶切技术在石油烃降解菌筛选鉴定中的应用 |
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| 引用本文： | 郝旭光,孙寓姣,王红旗.PCR-酶切技术在石油烃降解菌筛选鉴定中的应用[J].环境工程学报,2010,4(2):449-452. |
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| 作者姓名： | 郝旭光 孙寓姣 王红旗 |
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| 作者单位： | 北京师范大学水科学研究院,教育部水沙科学重点实验窒,北京,100875 |
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| 基金项目： | 国家高技术研究发展计划（863）项目（2007AA06Z308） |
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| 摘 要： | 将以原油驯化所得的纯培养微生物菌株作为模板,采用PCR(聚合酶链式反应)法,用扩增细菌16S rDNA的一对通用引物(8 f,1492 r)对模板扩增,并用限制性内切酶R saI和M spI对PCR产物进行ARDRA多态性分析。聚类去除重复菌株后60株菌株可分为11种不同分类操作单元(OTU),同时得出每个分类操作单元的数量。实验结果表明,此方法可快速去除重复菌株并反映出菌株间的系统进化关系;同时实验数据可构建成库,使后续分离菌株的筛选工作只需比对数据即可完成。
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| 关 键 词： | 聚合酶链式反应（PCR） 限制性内切酶 ARDRA 石油降解菌 16S rDNA |
Application of PCR-ARDRA technology in screening and identifiying oil degrading strains |
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| Hao Xuguang,Sun Yujiao and Wang Hongqi.Application of PCR-ARDRA technology in screening and identifiying oil degrading strains[J].Techniques and Equipment for Environmental Pollution Control,2010,4(2):449-452. |
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| Authors: | Hao Xuguang Sun Yujiao and Wang Hongqi |
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| Institution: | Key Laboratory for Water and Sediment Sciences, Ministry of Education, College of Water Sciences, Beijing Normal University, Beijing 100875, China,Key Laboratory for Water and Sediment Sciences, Ministry of Education, College of Water Sciences, Beijing Normal University, Beijing 100875, China and Key Laboratory for Water and Sediment Sciences, Ministry of Education, College of Water Sciences, Beijing Normal University, Beijing 100875, China |
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| Abstract: | In this study,pure culture microbial strains,which were acclimated by crude oil,were used as templates.A way of screening and identifying of oil-degrading bacteria was established,using PCR technique(premier 8f,1492r) and ARDRA patterns which used enzyme digestion(RsaI and MspI) of cloned 16S rDNA gene sequences.The results showed that 60 strains could be divided into 11 operational axonomic unit(OTU).And the ecological characteristics of these cloning communities could be analyzed by this method.The experi... |
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| Keywords: | PCR restriction endonuclease ARDRA oil degrading strains 16S ribosomal DNA |
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