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除草剂苯噻草胺污染对多食鞘氨醇杆菌(Sphingobacterium multivolum)抗氧化酶和ATP酶的影响
引用本文:叶央芳,闵航,吕镇梅.除草剂苯噻草胺污染对多食鞘氨醇杆菌(Sphingobacterium multivolum)抗氧化酶和ATP酶的影响[J].环境科学学报,2006,26(1):151-156.
作者姓名:叶央芳  闵航  吕镇梅
作者单位:1. 宁波大学生命科学与生物工程学院,宁波,315211;浙江大学生命科学学院,杭州,310029
2. 浙江大学生命科学学院,杭州,310029
基金项目:浙江省自然科学基金 , 中国科学院资助项目 , 宁波大学校科研和教改项目
摘    要:研究了暴露于苯噻草胺中的多食鞘氨醇杆菌Y1菌株中超氧化物歧化酶(SOD)、过氧化物酶(CAT)和ATP酶活性在短期内的变化.结果表明,苯噻草胺对细菌SOD活性诱导作用明显,尤其对处于对数生长期的细菌所产生的影响大于稳定生长期的细菌.苯噻草胺对CAT也有强烈的诱导作用,对处于不同生长阶段细菌的影响类似于对SOD的影响结果.苯噻草胺能激活ATP酶,但在细菌生长阶段影响较小.抗氧化酶和ATP酶相结合作为该类化合物对土壤污染胁迫的敏感生物指示物具有一定的可行性.

关 键 词:苯噻草胺  分子生态  超氧化物歧化酶(SOD)  过氧化物酶(CAT)  ATP酶
文章编号:0253-2468(2006)01-0151-06
收稿时间:2005/5/30 0:00:00
修稿时间:2005年5月30日

Effect of herbicide mefenacet pollution on antioxidant enzyme and ATPase of Sphingobacterium multivorum Y1
YE Yangfang,MIN Hang and L&#; Zhenmei.Effect of herbicide mefenacet pollution on antioxidant enzyme and ATPase of Sphingobacterium multivorum Y1[J].Acta Scientiae Circumstantiae,2006,26(1):151-156.
Authors:YE Yangfang  MIN Hang and L&#; Zhenmei
Institution:1. College of Life Science and Biotechnology, Ningbo University, Ningbo 315211 2. College of Life Science, Zhejiang University, Hangzhou 310029,College of Life Science, Zhejiang University, Hangzhou 310029 and College of Life Science, Zhejiang University, Hangzhou 310029
Abstract:The effect of exposed to mefenacet in short term on the activities of superoxide dismutase (SOD), catalase (CAT) and ATPase of Sphingobacterium multivorum Y1 capable of degrading mefenacet was studied. The results showed that mefenacet induced obviously SOD activity in S. multivorum Y1, especially to those in exponential phase, and also greatly stimulated CAT activity. The stimulation process of CAT activity was similar to that of SOD activity at different growth phases in S. multivorum Y1 by mefenacet. The level of ATPase activity increased after application of mefenacet, but there existed no good correlation with the growth phase of bacteria. It was feasible definitely to use antioxidant enzyme combined with ATPase as a biomarker for pollution stress of these chemicals in soil environment.
Keywords:mefenacet  molecular ecology  superoxide dismutase (SOD)  catalase (CAT)  ATPase
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