| 甲醛降解细菌的分离鉴定和降解效果测定 |
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| 引用本文： | 杨郁,金志华,李宁慧,金飞玲,黄翔和.甲醛降解细菌的分离鉴定和降解效果测定[J].环境工程学报,2009,3(8):1529-1531. |
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| 作者姓名： | 杨郁 金志华 李宁慧 金飞玲 黄翔和 |
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| 作者单位： | 1. 浙江大学宁波理工学院生物与化学工程分院,宁波,315100
2. 浙江省诸暨市教育局,诸暨,311800 |
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| 基金项目： | 宁波市自然科学基金项目（2006A610072） |
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| 摘 要： | 从某化工厂污水处理系统的活性污泥中分离纯化得到一株能降解甲醛的革兰氏阳性菌，对其进行形态及生理生化特性测定，提取并纯化其基因组DNA，通过PCR扩增菌株的16S rDNA，测序结果表明，该菌株和苏云金芽孢杆菌的同源性为100%。在含1.2 μL/mL的甲醛的培养基上培养该菌36 h后，甲醛降解去除率达到72％以上。
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| 关 键 词： | 甲醛 分离鉴定 16S rDNA PCR |
Isolation,identification and degradation effect of a formaldehyde-degrading bacteria |
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| Yang Yu,Jin Zhihu,Li Ninghui,Jin Feiling and Huang Xianghe.Isolation,identification and degradation effect of a formaldehyde-degrading bacteria[J].Techniques and Equipment for Environmental Pollution Control,2009,3(8):1529-1531. |
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| Authors: | Yang Yu Jin Zhihu Li Ninghui Jin Feiling and Huang Xianghe |
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| Institution: | 1.Department of Biotechnology and Chemical Engineering;Ningbo Institute of Technology;Zhejiang University;Ningbo 315100;China;2.Bureau of Education of Zhuji Zhejiang;Zhuji 311800;China |
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| Abstract: | A gram positive bacteria strain that can degrade formaldehyde was isolated from activated sludge of wastewater treatment plant from a chemical corporation.The strain was observed through structure and physio-logical and biochemical characteristics.Molecule identification methods including distilled DNA.PCR amplifiea-tion,production sequence showed that it was similar to Bacillus and 16S rDNA sequence shared 100%homology with that of Bacillus thuringiensis.Formaldehyde(1.2 μL/mL)was degraded above 72% with 36 h by this strain. |
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| Keywords: | 16S rDNA PCR formaldehyde isolation and identification 16S rDNA PCR |
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