苯酚对厌氧氨氧化工艺耦合反硝化的启动及脱氮性能的影响

研究了ANAMMOX耦合异养反硝化反应器的启动过程,考察了苯酚浓度对耦合反应器脱氮性能的影响.接种2L(占反应器有效容积的20%)挥发性悬浮固体(MLVSS)为6000mg/L的ANAMMOX颗粒污泥,在pH7.8、温度为25℃、HRT为1.5h的条件下经过86d的培养,ANAMMOX耦合异养反硝化启动成功.实验结果表明,在稳定运行阶段,NH4+-N、NO2--N和TN平均去除率分别为85.4%、86.1%和79.9%,TN平均容积负荷和TN平均去除负荷分别为2.63,2.10kg/(m3·d);ANAMMOX颗粒污泥外面包裹着苯酚反硝化菌;系统内异养反硝化与ANAMMOX存在协同和竞争关系.当苯酚浓度≥0.3mmol/L时,ANAMMOX菌的活性受到很大抑制,苯酚浓度的升高加剧了苯酚反硝化菌与ANAMMOX菌之间的竞争;从脱氮效果及系统稳定两方面综合考虑,当苯酚浓度为0.2mmol/L时,耦合效果最好,消耗的NH4+-N、NO2--N与生成的NO3--N之比为1:1.52:0.11.

Effect of phenol on startup and nitrogen removal performance of ANAMMOX coupling denitrifying

The startup of ANAMMOX coupling heterotrophic denitrifying reactor and the influence of concentration of phenol on the nitrogen removal performance of coupled reactor were investigated. Cultivated with 2L (20% of effective volume of reactor) anaerobic ammonia oxidation granular sludge that the volatile suspended solids (MLVSS) was 6000mg/L, anaerobic ammonia oxidation coupling heterotrophic denitrifying was started up successfully for 86days under the condition of pH 7.8, water temperature 25℃ and hydraulic detention time (HRT) 1.5h. The steady phase, the average removal rates of NH4+-N、NO2--N and TN were 85.39%,86.12% and 79.85% respectively, the average volumetric load of total nitrogen and the average removal load of total nitrogen were 2.63kg/(m3·d) and 2.10kg/(m3·d) respectively. ANAMMOX granular sludge was coated with phenol denitrifying bacteria. The relationship between heterotrophic denitrification and anammox was cooperative and competitive in the system. The activity of ANAMMOX bacteria was suppressed strongly when the concentration of phenol30.3mmol/L, the competition between phenol denitrifying bacteria and ANAMMOX bacteria was intensified as the increasing of concentration of phenol.When the concentration of phenol was 0.2mmol/L, the denitrification effect of system is best considering from denitrification and the stability of system. The ratio of removed NH4+-N, reomved NO2--N and generated NO3--N was 1:1.52:0.11.