针铁矿强化剩余污泥与烟草废弃物厌氧发酵的研究

以剩余污泥和烟草废弃物为研究对象，探究了针铁矿对有机物厌氧消化产甲烷的影响.针铁矿投加能提高消化产气率至359.4mL/g，显著高于空白对照组.针铁矿有助于混合气体中甲烷含量的升高并减少CO₂的体积分数.针铁矿利于剩余污泥和烟草废弃物共消化过程有机物溶出，纤维素减量，从而为产甲烷古菌提供充足的消化底物.针铁矿利于酸化代谢过程，在针铁矿投加组中挥发性脂肪酸的最大产量仅为56.2mg/g，显著低于空白组，此外针铁矿促进丙酸的合成.针铁矿促进乙酸降解，在消化第1d，针铁矿投加实验组中乙酸降解率高达65.5%，较高于空白组中的56.6%.酶活性分析表明针铁矿促进水解，酸化及产甲烷过程关键酶.Methanothrix和Methanobacterium是产甲烷阶段重要微生物，454高通量测序表明针铁矿提高Methanothrix和Methanobacterium的相对丰度至59.6%和23.5%，显著高于空白对照组.

Enhanced anaerobic fermentation of waste activated sludge and tobacco waste using Goethite

The effect of goethite on methane production by anaerobic digestion of organic matter was studied with waste activated sludge and tobacco waste as research objects. The addition of goethite increased digestive gas production rate to 359.4mL/g, which was significantly higher than that in the blank. Goethite contributed to the increase of methane content in mixed gases and reduced the volume fraction of CO₂. Goethite was conducive to the dissolution of organic matter during co-digestion of waste activated sludge and tobacco waste, and cellulose decreases to provide sufficient digestive substrate for methanogenesis Archaea. Goethite was beneficial to acidification metabolism. The maximum yield of volatile fatty acids in goethite group was only 56.2mg/g, which was significantly lower than that in blank. In addition, goethite promoted the synthesis of propionic acid. Goethite promoted the degradation of acetic acid. On the first day of digestion, the degradation rate of acetic acid reached 65.5% in the goethite group, which was higher than 56.6% in the blank. Enzyme activity analysis indicated that goethite promoted the key enzymes of hydrolysis, acidification and methanogenesis. Methanothrix and Methanobacterium are the important microorganisms in methanogenesis stage, and 454high-throughput sequencing showed that goethite increased the relative abundance of Methanothrix and Methanobacterium to 59.6% and 23.5%.