| 不同TOC/NH4+-N对厌氧氨氧化脱氮效能的影响 |
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| 引用本文： | 王凡,刘凯,林兴,周正,李祥,黄勇.不同TOC/NH4+-N对厌氧氨氧化脱氮效能的影响[J].环境科学,2017,38(8):3415-3421. |
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| 作者姓名： | 王凡 刘凯 林兴 周正 李祥 黄勇 |
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| 作者单位： | 苏州科技大学环境科学与工程学院, 苏州 215009;苏州科技大学环境生物技术研究所, 苏州 215009,苏州科技大学环境科学与工程学院, 苏州 215009;苏州科技大学环境生物技术研究所, 苏州 215009,苏州科技大学环境科学与工程学院, 苏州 215009;苏州科技大学环境生物技术研究所, 苏州 215009,苏州科技大学环境科学与工程学院, 苏州 215009;苏州科技大学环境生物技术研究所, 苏州 215009,苏州科技大学环境科学与工程学院, 苏州 215009;苏州科技大学环境生物技术研究所, 苏州 215009,苏州科技大学环境科学与工程学院, 苏州 215009;苏州科技大学环境生物技术研究所, 苏州 215009 |
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| 基金项目： | 国家重点研发计划项目（2016YFC0401103）；国家自然科学基金项目（51478284，51408387）；江苏省特色优势学科二期项目；江苏省水处理技术与材料协同创新中心项目 |
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| 摘 要： | 采用SBR厌氧氨氧化反应器,研究了不同TOC与NH_4~+-N比值对厌氧氨氧化反应器的脱氮效能的长短期影响.结果表明,在有机物短期影响时,反应器所能承受的最大TOC/NH_4~+-N为1.4,总氮去除速率可达0.26 kg·(m~3·d)~(-1).长期影响下,在TOC/NH_4~+-N小于0.4时,反应器可获得最高脱氮效能,总氮去除率为0.34 kg·(m~3·d)~(-1),TOC/NH_4~+-N大于0.4后,反应器脱氮效能持续降低,并且短期内厌氧氨氧化菌难以迅速恢复活性.利用q PCR(定量PCR)技术对长期影响前后反应器内菌种群落变化做定量分析,结果表明随着有机物的增加,反应器中的ANAMMOX菌数量从2.9×10~(11)copies·mL~(-1)减少至3.15×10~(10)copies·mL~(-1),在TOC/NH_4~+-N大于1.6的环境中,NH_4~+-N未能由厌氧氨氧化菌去除,厌氧氨氧化菌不能表现出生物活性.此时测得反硝化菌数量为3.0×10~9copies·mL~(-1),反应器中的NO_2~--N绝大部分由反硝化去除,虽然反硝化菌数量远少于ANAMMOX菌,但能表现出远超ANAMMOX菌的活性.
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| 关 键 词： | 厌氧氨氧化 反硝化 有机物 TOC/NH4+-N 定量PCR |
| 收稿时间： | 2017/1/21 0:00:00 |
| 修稿时间： | 2017/2/24 0:00:00 |
Effect of Different TOC to NH4+-N Ratios on Nitrogen Removal Efficiency in the ANAMMOX Process |
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| WANG Fan,LIU Kai,LIN Xing,ZHOU Zheng,LI Xiang and HUANG Yong.Effect of Different TOC to NH4+-N Ratios on Nitrogen Removal Efficiency in the ANAMMOX Process[J].Chinese Journal of Environmental Science,2017,38(8):3415-3421. |
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| Authors: | WANG Fan LIU Kai LIN Xing ZHOU Zheng LI Xiang and HUANG Yong |
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| Institution: | School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China,School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China,School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China,School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China,School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China and School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China |
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| Abstract: | The effect of the TOC to NH4+-N ratio on nitrogen removal rate in an ANAMMOX sequencing batch reactor was studied for the short term and long term. Short-term results showed the maximum TOC/NH4+-N that ANAMMOX could afford was 1.4. At the same time, the rate of nitrogen removal was 0.26 kg·(m3·d)-1. The maximum nitrogen removal rate of 0.34 kg·(m3·d)-1 was obtained when TOC/NH4+-N was less than 0.4 in the long-term experiment. Whereas the nitrogen removal rate of the reactor decreased continuously when TOC/NH4+-N exceeded 0.4, and it was difficult to recover the activity of the ANAMMOX bacteria in the short term. qPCR results showed that the amount of ANAMMOX bacteria dropped from 2.9×1011 copies·mL-1 to 3.15×1010 copies·mL-1 with the increase of organics. When TOC/NH4+-N exceeded 1.6, NH4+-N could hardly be removed by the ANAMMOX bacteria, which were severely inhibited. The number of denitrifying bacteria was 3.0×109 copies·mL-1; meanwhile, most of the NO2--N in the reactor was removed via denitrification. |
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| Keywords: | ANAMMOX denitrification organic matter TOC/NH4+-N quantitative PCR |
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