| 生活垃圾厌氧堆肥产甲烷及古细菌多样性分析 |
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| 引用本文： | 闫江,江娟.生活垃圾厌氧堆肥产甲烷及古细菌多样性分析[J].环境科学与技术,2006,29(4):9-10,18. |
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| 作者姓名： | 闫江 江娟 |
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| 作者单位： | 1. 华中科技大学生命科学与技术学院
2. 华中科技大学环境科学与工程学院,武汉,430074 |
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| 基金项目： | 湖北省武汉市科技攻关项目 |
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| 摘 要： | 通过厌氧堆肥试验,对厌氧堆肥产甲烷的基本特征进行了研究,结果表明:在厌氧堆肥开始阶段,气体中只有8%的甲烷,二氧化碳产率是甲烷产率的4倍左右;而随着反应的进行,二氧化碳产率呈下降趋势,甲烷产率逐渐升高,并于3个月时达到最高值45%;此后二氧化碳及甲烷产率都逐渐降低。对3个月时的垃圾堆肥渗出液取样,提取总DNA,对古细菌片段进行限制性片段长度多样性分析(RFLP),在60个随机选出的古细菌rDNA克隆子中,可以划分15个不同的谱型。对深入了解产甲烷厌氧微生物过程,加快垃圾稳定化具有重要意义。
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| 关 键 词： | 生活垃圾 厌氧 堆肥 甲烷 古细菌 RFLP分析 |
| 文章编号： | 1003-6504（2006）04-0009-03 |
| 收稿时间： | 2005-03-31 |
| 修稿时间： | 2005-03-312005-08-23 |
Features of Methane Produced in Anaerobic Digestion and Analysis of Archaea Diversity |
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| YAN Jiang,JIANG Juan.Features of Methane Produced in Anaerobic Digestion and Analysis of Archaea Diversity[J].Environmental Science and Technology,2006,29(4):9-10,18. |
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| Authors: | YAN Jiang JIANG Juan |
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| Abstract: | Bench-scale composting experiment was made to study the features of methane produced in anaerobic digestion. Results indicated that with the processing of reaction, the output of CO2 decreased while methane increased to the maximum of 45% by the end of 3 months. RFLP analysis was also used to analyze Archaeal 16S rDNA genes that were selectively amplified from total genomic DNA extracted from microbial community in the leachate of compost for 3 months. |
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| Keywords: | municipal solid waste anaerobic digestion composting methane Archaea RFLP analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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