应用生物酶法提取黄姜皂素的清洁工艺研究

文章采用生物复合酶法对薯蓣皂素进行提取研究,使黄姜淀粉和纤维素得到充分降解,促进葡萄糖与皂素的分离,从而有利于黄姜皂素的提取.通过正交实验进行试验确定复合酶处理黄姜皂素的工艺,即最适酶解条件为温度55℃,pH值4.0,时间6 h,每克黄姜酶用量为0.03 g,通过该条件进行试验发现,黄姜皂素收率提高26%.同时该工艺与...

Application of Complex Enzyme in Extraction of Yam Sapogenins

Application of complex enzyme in extraction of Dioscorea sapogenins was studied.The starch and cellulose in yam were completely hydrolyzed and easily separated for the extraction of diosgenin from glucose catabolizing solution.Technical parameters of the complex enzyme extraction by the orthogonal test were determined,with the optimal parameter for extraction as hydrolysis temperature 55 ℃,pH of solution 4.0,time of enzymatic hydrolysis 6 h and amount of enzymatic hydrolysis 0.03 g per Dioscorea zingiberensis.Results showed that diosgenin extraction ratio of such method was raised by 26%.Compared with the traditional method,acid quantity decreased by 74.7%,wastewater quantity and COD concentration declined by 64.1% and 89.6% respectively,with high melting point for diosgenin product.Results showed that the technology is featured by gentle function condition,higher efficiency,less pollution and energy saving,which would remarkably decrease the sewage quantity and successfully solve environment pollution in yam diosgenin production.The technology is a cleaner process and has a bright prospective.