| 微囊藻毒素分离及鉴定 |
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| 引用本文： | 余婷,谢平,戴明,梁高道.微囊藻毒素分离及鉴定[J].环境科学与技术,2010,33(1). |
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| 作者姓名： | 余婷 谢平 戴明 梁高道 |
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| 作者单位： | 中国科学院水生生物研究所,湖北,武汉,430072 |
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| 基金项目： | 国家自然科学基金项目(30530170) |
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| 摘 要： | 微囊藻毒素是一类由蓝藻产生的具有肝毒性的生物毒素。微囊藻毒素标准品是开展为微囊藻毒素相关研究的必需的实验材料,文章以滇池天然水华蓝藻为原料,建立了以5%乙酸和75%的甲醇溶液提取,通过优化提取、分离和制备条件,制备了一定量的微囊藻毒素高纯度样品,经HPLC鉴定分析,纯度可达90%以上。文章在相同的质谱条件下分别对MC-LR和Dha7]MC-LR等毒素进行了质谱检测,一级质谱结果表明,MC-LR和Dha7]MC-LR的一价电离离子峰分别为995和981,MC-RR的二价电离离子峰为520,并分析它们的二级质谱裂解特征,确定三种毒素化学结构MC-RR为环(Ala-Arg-MeAsp-Arg-Adda-Glu-Mdha)、MC-LR为环(Ala-Leu-MeAsp-Arg-Adda-Glu-Mdha)、Dha7]MC-LR为环(Ala-Leu-MeAsp-Arg-Adda-Glu-Dha)。改良后的检测方法同样应用于检测受微囊藻毒素污染的武汉东湖水样。
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| 关 键 词： | 微囊藻毒素 提取 制备 高效液相色谱-质谱检测 |
Isolation and Identification of Microcystins |
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| YU Ting,XIE Ping,DAI Ming,LIANG Gao-dao.Isolation and Identification of Microcystins[J].Environmental Science and Technology,2010,33(1). |
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| Authors: | YU Ting XIE Ping DAI Ming LIANG Gao-dao |
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| Institution: | Institute of Hydrobiology;Chinese Academy of Sciences;Wuhan 430072;China |
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| Abstract: | Microcystins are a kind of hepatotoxic cyanotoxin.Extraction of intracellular Microcystin variants is required to characterize and quantify all microcystins present in a sample.The method was established to isolate and purify Microcystins from natural cyanobacterial bloom dominated by Microcystis aeruginosa in Dianchi Lake,Yunnan Province,by the main steps of extraction with 75% aqueous methanol,5% acetic acid solution through semi-prep-LC and ODS column with purity over 90% .The study was to develop an app... |
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| Keywords: | Microcystins extraction preparation high performance liquid chromatography-mass spectrometry |
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