| 高氯酸盐降解菌的分离鉴定及特性研究 |
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| 引用本文： | 吴春笃,郭静,许小红.高氯酸盐降解菌的分离鉴定及特性研究[J].生态环境,2010,19(2):281-285. |
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| 作者姓名： | 吴春笃 郭静 许小红 |
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| 作者单位： | 1. 江苏大学环境学院,江苏,镇江212003;扬州环境资源职业技术学院,江苏,扬州,225002
2. 江苏大学环境学院,江苏,镇江212003 |
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| 基金项目： | 国家自然科学基金项目，江苏大学高级人才基金资助项目 |
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| 摘 要： | 研究降解高氯酸盐环境污染物微生物的形态特征、分子系统特征、生长特性和降解活性。利用PCA选择性培养基富集分离高氯酸根降解菌,观察其形态,进行生理生化测定,用分子克隆技术获得菌株的16SrDNA基因并测序,并对菌株的16S rDNA基因序列进行比对和系统发育分析,并用培养技术和离子色谱法检测菌株对底物的利用和降解情况。从镇江江滨和镇江新区污水处理厂的活性污泥中分别分离到一株能降解高氯酸根的菌株JD14和JD125。此菌株在24～30℃条件下较快地降解底物高氯酸盐,其中在24℃下,15d内可以将初始质量浓度为1600mg·kg-1的高氯酸根降解（86±6.5）%。对两菌株的16S rDNA基因进行了克隆和测序,并进行系统发育分析,结果表明菌株JD14和Dechloromonas sp.SIUL相似度高达100%,JD125和Dechlorospirillum相似度达97%,最后鉴定JD14和JD125属于高氯酸盐降解菌。此前国内并无降解高氯酸根微生物的报道,对今后在环境污染防治中开发利用此类细菌具有指导意义。
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| 关 键 词： | 高氯酸盐还原降解菌 16S rDNA基因 生长曲线 生物降解 |
Isolation and identification of perchlorate-degrading bacteria and its characteristics |
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| WU chundu,GUO jing,XU Xiaohong.Isolation and identification of perchlorate-degrading bacteria and its characteristics[J].Ecology and Environmnet,2010,19(2):281-285. |
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| Authors: | WU chundu GUO jing XU Xiaohong |
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| Institution: | WU chundu1,2,GUO jing1,XU Xiaohong1 1.School of environment,JiangsuUniversity,Zhenjiang,Jiangsu 212013,China,2.Yangzhou vocational College of environment , resources,Yangzhou,Jiangsu 225002 |
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| Abstract: | Objective It was to study the morphological characteristics,molecular phylogeny of microbes,growth characteristics and catabolic capability degrading environmental pollutants such as perchlorate.Methods a selective PCA medium was used to isolate Perchlorate-Reducing bacterium,observing their morphology,routine physiological and biochemical experiment,molecular cloning technique was used to obtain the 16S rDNA gene then they were sequenced,aligned the relative 16S rDNA gene sequences and constructed the phyl... |
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| Keywords: | Perchlorate-Reducing bacterium 16S rDNA gene growth curve biodegradation |
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