重金属Cd、Pb对褐菖鲉肝脏组织中HSPs基因表达的影响

为了解重金属污染对海洋鱼类热休克蛋白(HSPs)基因表达的影响,将褐菖鲉(Sebastiscus marmoratus)分别暴露于1.6、8、40、200、500μg·L~(-1)Cd、Pb溶液中,用环介导等温扩增技术(loop-mediated isothermal amplification,LAM P)定量检测褐菖鲉肝脏HSP60、HSP70、HSP90、HSC70 mRNA表达量。结果表明:Pb仅在40μg·L-1时显著抑制HSP60、HSP90、HSC70 mRNA表达量,8μg·L~(-1)时即可显著抑制HSP70 m RNA表达量,并在40μg·L~(-1)时达到最小值;Cd对HSP60、HSP90、HSC70的诱导不明显,但能显著诱导HSP70,并在500μg·L~(-1)时达到最大值。相比之下,褐菖鲉肝HSP70基因对重金属Cd、Pb污染较为敏感,有潜力成为监测海洋重金属污染的预警分子。

The Effects of Cd and Pb on the Levels of Hepatic HSPs mRNA in Sebastiscus marmoratus

In order to understand the effects of heavy metal pollution on the gene expression of heat shock proteins (HSPs) in marine fish, Sebastiscus marmoratus were exposed to different concentrations (i.e., 1.6, 8, 40, 200, 500μg?L￣1) of Cd or Pb for 5 days. A quantitative loop￣mediated isothermal amplification (LAMP) method was used to analyze the hepatic mRNA levels of HSP60, HSP70, HSP90, and HSC70. Under the Pb exposure, the mRNA levels of hepatic HSP60, HSP90, HSC70 were not significantly reduced except in the 40μg?L￣1 treatment, whereas the mRNA levels of HSP70 were significantly decreased by 8μg?L￣1 Pb, and reached the minimum when Pb was 40 μg?L￣1 . Under the Cd exposure, the mRNA levels of hepatic HSP60, HSP90, HSC70 were not significantly af￣fected;in contrast, mRNA levels of HSP70 were significantly increased and peaked at 500 μg?L￣1 Cd. In conclu￣sion, the hepatic HSP70 was relatively more sensitive to heavy metal exposure than the other HSPs, and thus had the potential to be the early warning for heavy metal pollution in marine environment.