Isolation and characterization of 1,2,4-trichlorobenzene mineralizing Bordetella sp. and its bioremediation potential in soil

A soil which has been polluted with chlorinated benzenes for more than 25 years was used for isolation of adapted microorganisms able to mineralize 1,2,4-trichlorobenzene (1,2,4-TCB). A microbial community was enriched from this soil and acclimated in liquid culture under aerobic conditions using 1,2,4-TCB as a sole available carbon source. From this community, two strains were isolated and identified by comparative sequence analysis of their 16S-rRNA coding genes as members of the genus Bordetella with Bordetella sp. QJ2-5 as the highest homological strain and with Bordetella petrii as the closest related described species. The 16S-rDNA of the two isolated strains showed a similarity of 100%. These strains were able to mineralize 1,2,4-TCB within two weeks to approximately 50% in liquid culture experiments. One of these strains was reinoculated to an agricultural soil with low native 1,2,4-TCB degradation capacity to investigate its bioremediation potential. The reinoculated strain kept its biodegradation capability: (14)C-labeled 1,2,4-TCB applied to this inoculated soil was mineralized to about 40% within one month of incubation. This indicates a possible application of the isolated Bordetella sp. for bioremediation of 1,2,4-TCB contaminated sites.     

THE INFLUENCE OF DRAW-DOWN ON RECREATION ON THE TRENT CANAL RESERVOIR-LAKES1

ABSTRACT: Water-based outdoor recreation constitutes an important benefit in many multiple-use projects. One problem frequently encountered, however, is the fluctuation of the water-level in a reservoir, especially if draw-downs occur during the summer recreation season. The Trent Canal in Ontario, Canada, forms a unique recreation waterway of some 240 miles of canal and navigable lakes and rivers. For the control of the water in the canal, the Canadian Federal government manages 44 reservoir-lakes as water-storage sites to augment the flows in the canal. While most disputes in water allocation for recreation generally involve recreation uses versus other, non-recreation uses, in the case of the Trent Canal and its reservoir-lakes the dispute in water allocation exists between two competing recreation-uses: for recreation boating on the Canal and its waterway, and for recreation use of the reservoir-lakes. Each use has its own duty-of-water in terms of requirements, or water conditions, for its optimum use. On the reservoir-lakes, by far the largest recreation user-group, is made up of the owners of private shoreline summer cottages (cabins). Draw-downs on the reservoir-lakes, in some cases as much as 13 feet, hence constitute a negative externality imposed by the canal, especially since water-level lowering takes place continuously throughout the summer. In-depth interviews conducted with over 100 cottage-owners indicate four main conclusions. First, the previous user assumptions of the extent of the negative effect of water-level fluctuation on recreation had been exaggerated. Only in extreme draw-downs during infrequent dry years would draw-down constitute a serious imposition. Second, water-level fluctuation does constitute a form of inconvenience to the private riparian recreation property owners, but it does not generally reduce the quantity of recreation use of the reservoir-lakes. Third, there is evidence that reservoir-lake users have learned to adapt successfully to water-level draw-downs. Fourth, the shoreline physiography of the location of a riparian owner strongly influences the way in which draw-down affects attitudes and opinions. The complex jurisdictional split of the land and water resources of the region points to the need to establish a Trent Canal Authority, to oversee the proper use and development of the canal, its waterway, and the reservoir-lakes.     

Wireless charging and shared autonomous battery electric vehicles (W+SABEV): synergies that accelerate sustainable mobility and greenhouse gas emission reduction

Mitigation and Adaptation Strategies for Global Change - Emerging technologies play important roles in shaping future mobility systems and impacting sustainability performance of the transportation...     

Teil I: Braucht die Landwirtschaft Umweltmanagementsysteme?

The farming community is today faced with requests by various interest groups to implement environmental management systems (EMS). The series of items shows principle theoretical questions of the method and the advantages of EMS (part I and II), presents the practical outcome (part III) and give a recommendation for potential users of EMS in agricultural systems (part IV). The study conducted outlines the method of the implementing an EMS according to the Environmental Management and Audit System (EMAS) and ISO 14001. Further advantages and disadvantages for the agricultural industry are addressed. Even though terms such as site can not easily be defined or problems with open material cycles and structural particularity of agricultural farms there appears to be a benefit to implement. The transparency and documentation of the material flow as well as the economical benefits of an optimized organization count towards the EMS. Further EMS can be a means to strengthen the argumentative position for example with the public, authorities, banks or insurance. This concurs with minimizing company liabilities and obtaining governmental privileges (deregulation).     

Stakeholder attitudes on Carbon Capture and Storage—An international comparison

This paper presents results from a survey of stakeholder attitudes towards Carbon Capture and Storage (CCS). The survey is the first to make a global comparison across three major regions: North America, Japan, and Europe. It is based on a 30-question survey which targeted individuals working at stakeholder organizations that seek to shape, and will need to respond to, policy on CCS, including electric utilities, oil and gas companies, CO₂-intensive industries and non-governmental organizations (NGOs). The paper reports results from the original survey carried out in 2006 and from a recent follow up on key CCS questions (April 2009).The results show generally small differences across the regions and between the different groups of stakeholders. All believed that the challenge of significant reductions in emissions using only current technologies was severe. There was a widespread belief that CCS as well as renewable technologies such as solar power will achieve major market entry into the electricity sector within the next 10–20 years, whereas there is more scepticism about the role of hydrogen and especially nuclear fusion in the next 50 years. All groups were generally positive towards renewable energy. Yet, there were some notable areas of disagreement in the responses, for example, as expected, NGOs considered the threat of climate change to be more serious than the other groups. North American respondents were more likely to downplay the threat compared to those of the other regions. The Japanese were more concerned about the burden that would be placed on industry in the coming decade as a result of emissions constraints and NGOs were more likely to believe that the burden imposed would be light or very light. NGO respondents also believed CCS to be far more attractive than nuclear power (fission) but much less than renewables. As expected, the risk for leakage from reservoirs was ranked number one of the risk options given. The follow-up study generally confirmed the results of the original study with a few notable differences. As expected, the results of the follow-up shows that respondents consider CCS to play an increased role in the national climate debate. In Japan, there was an increased fraction of respondents who claimed that their organization has a clear position on CCS.