长梗黄精主根与须根多糖的提取工艺研究

对长梗黄精主根与须根多糖的提取工艺进行了研究.在对乙醇浓度、浸提温度、浸提时间和粉碎粒度等进行单因素试验的基础上,通过正交试验确定了长梗黄精主根与须根多糖提取的最佳工艺条件,即对长梗黄精主根多糖的提取最佳条件为乙醇浓度80％、浸提温度80℃、浸提时间50min、颗粒大小40目,此时多糖提取率为18.754％;而对长梗黄精须根多糖的提取最佳条件为乙醇浓度70％、浸提温度60℃、浸提时间30min、颗粒大小80目,此时多糖提取率为16.652％.须根多糖含量与主根含量差异性不显著,故须根的利用价值也很高,有待开发利用.     

苯酚-硫酸法测定好氧颗粒污泥胞外聚合物中多糖浓度的研究

以葡萄糖为对照，优选苯酚-硫酸法测定胞外聚合物中多糖含量的显色条件，对此法精密度进行研究。结果表明，此测定方法显色稳定，重复性好，但回收率欠佳，测得的多糖含量要大于实际值。     

黄丝郁金不同采收期的产量和质量研究

目的:研究黄丝郁金不同采收期对产量和质量的影响,以确定适宜的采收时期。方法:设定10月、11月、12月、1月2、月5个不同采收时间,定期采收,测产,测试挥发油和姜黄素含量。结果:10—12月郁金产量有明显增加,12月至次年2月产量无明显差异;10—12月郁金的姜黄素和挥发油含量显著增加,12月至次年2月含量无明显差异。结论:总体看来,黄丝郁金的采收期以12月底为宜。     

人工湿地中氮代谢微生物的共存策略及机理

人工湿地中氮元素的脱除主要依靠多种微生物的联合作用，因此氮代谢微生物的和谐共存成为人工湿地高效脱氮的基础。本文分析回顾了氨氧化细菌、亚硝酸盐氧化菌、反硝化细菌及厌氧氨氧化菌等主要氮代谢微生物的共存策略及反应机理。     

雅砻江两河口水电站人工鱼类产卵场工程设计

两河口水电站工程建设改变了工程河段的自然生态环境,导致原有鱼类产卵场被部分淹没。修建人工鱼类产卵场是水生生态生境修复的重要措施之一。两河口人工鱼类产卵场位于库尾和平乡河段,总面积1.6hm2。根据流场数值模拟,生境适宜度分析成果,产卵场分成AB两个大区,其中A区为裂腹鱼类产卵场,B区为鮡科鱼类、鳅科鱼类产卵场。A区裂腹鱼类产卵场分为Ⅰ区,Ⅱ区,产卵对象分别为短须裂腹鱼、长丝裂腹鱼等裂腹鱼类以及厚唇裸重唇鱼与软刺裸裂尻鱼;B区鮡科鱼类、鳅科鱼类产卵场分为Ⅲ区与Ⅳ区,产卵对象分别为鮡科鱼类及鳅科鱼类。     

无花果曲霉菌丝球对活性艳蓝KN-R的脱色研究

生物吸附法是染料废水处理中很有前途的一种方法。本文将培养的无花果曲霉菌丝球用于蒽醌染料活性艳蓝KN-R的脱色，研究了培养时间、温度、转速、pH值及盐浓度、不同碳源、不同氮源对菌丝球脱色的影响；比较了活菌与死菌的脱色效果；探讨了菌丝球重复利用对脱色率的影响。结果表明：培养时间为72h，温度为33℃，转速为150rpm，pH值为6．0，盐浓度为0.5％，以乳糖为碳源、硝酸钠为氮源时，菌丝球对活性艳蓝KN-R的脱色效果最好：活菌对染料的吸附性能比死菌好；菌丝球在重复利用了四次后，脱色率仍达85．7％。     

桑金钱菌的纯化培养及多糖获取

采用组织分离法,从桑树上分离到一菌种,鉴定为担子菌亚门层菌纲伞菌目口蘑科冬菇属真菌类。经培养,该菌种适宜生长温度为22℃,适宜生长培养基为马铃薯培养基。在液体培养基中震荡培养得到大量菌丝,经冷冻干燥、微波提取后得粗多糖,含量为5.36%左右。     

青霉菌X5对活性艳蓝KN-R脱色研究

采用梯度平板筛选法，从染布厂废水池污泥中分离到一株对蒽醌染料KN－R有较强脱色能力的青霉菌X5（Penicillium sp.)，并考察该菌在不同温度，pH，培养时间，染料浓度，菌量及不同碳源氮源条件下对活性艳蓝KN－R的脱色情况，结果表明,在20－40℃温度范围内该菌对活性艳蓝KN－R的脱色率均在95％以上，其最佳脱色温度为25℃，pH为4．0，培养时间为48h，染料浓度，菌量对染料脱色有一定影响，该菌对碳源、氮源具有较宽适应范围，在最佳条件下，该菌对染料的脱色率达99．3％。     

采收前软枣猕猴桃“魁绿”果实理化性质变化研究

为了了解猕猴桃果实生长发育的规律,以软枣猕猴桃"魁绿"果实为材料,探究软枣猕猴桃"魅绿"的果实糖分、有机酸含量和果实形态的动态变化。结果显示:软枣猕猴桃"魅绿"的果实重量、纵横径积累呈"S"型曲线增长,果实淀粉积累时间短,在盛花期(DAA) 90天时达到最大值9. 75g/100gFW,果糖、葡萄糖和干物质含量呈线性增长。软枣猕猴桃"魅绿"的蔗糖和总可溶性糖从105DAA开始急剧上升,采收时生成少量麦芽糖。在45DAA前,软枣猕猴桃"魅绿"的VC含量持续增加,而柠檬酸和苹果酸浓度保持相对稳定,含量分别为4. 08g/100gFW、6. 17g/100gFW;柠檬酸在45—120DAA浓度保持持续增加,采收前轻微下降,而VC、苹果酸和奎宁酸浓度则随着果实发育而逐渐减低。     

海星多糖对小鼠酒精性脂肪肝损伤的预防研究

以多棘海盘车体壁为材料提取海星多糖,探讨多糖对小鼠酒精肝损伤的预防作用。采用水提法提取多糖,硫酸—苯酚法进行测定。以正常小鼠作空白对照,将酒精肝小鼠分为酒精肝模型组,海星多糖低、中、高剂量组(20mg/kg、40mg/kg、80mg/kg)进行灌胃,测定小鼠生理指标。结果显示,与模型组相比,海星多糖组小鼠血清中ALT、AST含量降低(P<0.05),TG、MDA含量也降低(P<0.05),GSH-PX、SOD活性升高(P<0.05),肝脏脂肪变性明显减轻,说明海星多糖对酒精性肝脏有较明显的保护作用。     

Conversion of temperate forests into heaths: Role of ecosystem disturbance and ericaceous plants

Fire and logging in nutrient-poor temperate forests with certain ericaceous understory plants may convert the forests into heaths. The process of disturbance-induced heath formation is documented by using examples ofCalluna in western Europe,Kalmia in Newfoundland, andGaultheria (salal) in coastal British Columbia. In a cool, temperate climate, rapid vegetative growth ofCalluna, Kalmia, and salal following disturbance results in increasing organic accumulation (paludification), nutrient sequestration, soil acidification, and allelochemicals. These are thought to be the main reasons to conifer regeneration failure in disturbed habitats. If continuation in forest is a land-use objective, then temperate forests with an ericaceous understory should not be logged unless effective silvicultural methods are devised to control the ericaceous plants and restore forest regeneration. Preharvest vegetation control may be considered as an option. Failure to control the understory plants may lead to a long-term vegetation shift, from forest to heathland, particularly in nutrient-poor sites. Successful methods of controllingKalmia andGaultheria, however, have yet to be developed. While theKalmia- andGaultheria- dominated heathlands are undesirable in Canada and the Pacific Northwest, a wide range ofCalluna heathlands of western Europe are being conserved as natural and seminatural vegetation.     

BIOLOGICAL INDICATORS AND BIOLOGICAL EFFECTS OF ESTUARINE/COASTAL POLLUTION1

ABSTRACT: Sustained interest in and concern about the health status of the aquatic environment has resulted in extensive research focused on (1) effects of pollution on survival, growth, and reproduction of resource species at all life stages; (2) diseases of fish and shellfish, as they may be related to pollution and as they may serve as indicators of environmental stress; and (3) contaminant body burdens in fish and shellfish - their effects on the aquatic animals and their potential effects on humans. Effects, lethal and sublethal, of pollutants on life history stages of fish and shellfish have been documented, as have impacts on local stocks in badly degraded habitats, but as yet there has been no adequate quantitative demonstration of effects on entire aquatic species - probably because of the difficulty in sorting out relative effects of the many environmental factors that influence abundance. Sublethal effects, especially those that result in disease, have been examined intensively, and some diseases and disease syndromes have been associated statistically with pollution. Other pollution indicators (biochemical, physiological, genetic, behavioral, and ecological) have also received some attention, as have body burdens of contaminants in aquatic species. Research, especially that conducted during the past decade, has done much to clarify the many pathways and toxic effects of contaminants on aquatic animals, and has also helped to identify mechanisms for survival of fish and shellfish in the presence of environmental changes caused by human activities.     

Using Paleo Reconstructions to Improve Streamflow Forecast Lead Time in the Western United States

In water stressed regions, water managers are exploring new horizons that would help in long‐range streamflow forecasts. Oceanic‐atmospheric oscillations have been shown to influence streamflow variability. In this study, long‐lead time streamflow forecasts are made using a multiclass kernel‐based data‐driven support vector machine (SVM) model. The extended streamflow records based on tree ring reconstructions were used to provide a longer time series data. Reconstructed data were used from 1658 to 1952 and the instrumental record was used from 1953 to 2007. Reconstructions for oceanic‐atmospheric oscillations included the El Niño‐Southern Oscillation, Pacific Decadal Oscillation, Atlantic Multidecadal Oscillation, and North Atlantic Oscillation. Streamflow forecasts using all four oscillations were made with one‐year to five‐year lead times for 21 gages in the western United States. This is the first study that uses both instrumental and reconstructed data of oscillations in SVM model to improve streamflow forecast lead time. SVM model was able to provide “satisfactory” to “very good” forecasts with one‐ to five‐year lead time for the selected gages. The use of all the oscillation indices helped in achieving better predictability compared to using individual oscillations. The SVM modeling results are better when compared with multiple linear regression model forecasts. The findings are statistical in nature and are expected to be useful for long‐term water resources planning and management.     

Journalists' views of advertiser pressures on agricultural news

All major journalism ethical codes explicitly state that journalists should protect editorial copy from undue influence by outside sources. However, much of the previous research on agricultural information has concentrated on what information various media communicate (gatekeeping studies) or communication's role in increasing innovation adoption (diffusion studies). Few studies have concentrated specifically on organizational and structural constraints that might adversely affect agricultural journalists' ethical standards; those that have, focus largely on farm magazines. A study of newspaper reporters who cover agricultural news found that the most pressing ethical concern is the effect of advertiser (agri-business) pressure on editorial copy, and that their concerns in general parallel those of farm magazine writers and editors. The majority reported being in situations in which they might be exposed to advertiser pressure, including pressures to change or withhold editorial copy. Large minorities suggested that advertising pressures affect the overall environment in which agricultural journalists work, and more than one in ten said they allow advertiser pressures to influence editorial decisions. The newspaper reporters who cover agricultural beats showed slightly more resistance to advertiser pressure than did farm magazine editors in a parallel study.     

Definition Procedures Have Little Effect on Performance of Environmental Classifications of Streams and Rivers

Mapped environmental classifications are defined using various procedures, but there has been little evaluation of the differences in their ability to discriminate variation in independent ecological characteristics. We tested the performance of environmental classifications of the streams and rivers of France that had been defined from the same environmental data using geographic regionalization and numerical classification of individual river valley segments. Test data comprised invertebrate assemblages, water chemistry, and hydrological indexes obtained from sites throughout France. Classification performance was measured by analysis of similarity (ANOSIM). Geometric regions defined by a regular grid and without regard to environmental variables and a posteriori classifications based on clustering the test datasets defined lower and upper bounds of performance for a given number of classes. Differences in classification performances were generally small. The ANOSIM statistics for the a posteriori classifications were around twice that of all environmental classifications, including geometrically defined regions. The hydro-ecoregions performed slightly better for the invertebrate data and the network classification performed slightly better for the chemistry and hydrological data. Our results indicate that environmental classifications that are defined using different procedures can be comparable in terms of their ability to discriminate variation of ecological characteristics and that alleged differences in performance arising from different classification procedures can be small relative to unexplained variation. We conclude that definition procedures might have little effect on the performance of large-scale environmental classifications and decisions over which procedures to use should be based primarily on pragmatic considerations.     

Urban ecological systems: scientific foundations and a decade of progress

Urban ecological studies, including focus on cities, suburbs, and exurbs, while having deep roots in the early to mid 20th century, have burgeoned in the last several decades. We use the state factor approach to highlight the role of important aspects of climate, substrate, organisms, relief, and time in differentiating urban from non-urban areas, and for determining heterogeneity within spatially extensive metropolitan areas. In addition to reviewing key findings relevant to each state factor, we note the emergence of tentative "urban syndromes" concerning soils, streams, wildlife and plants, and homogenization of certain ecosystem functions, such as soil organic carbon dynamics. We note the utility of the ecosystem approach, the human ecosystem framework, and watersheds as integrative tools to tie information about multiple state factors together. The organismal component of urban complexes includes the social organization of the human population, and we review key modes by which human populations within urban areas are differentiated, and how such differentiation affects environmentally relevant actions. Emerging syntheses in land change science and ecological urban design are also summarized. The multifaceted frameworks and the growing urban knowledge base do however identify some pressing research needs.     

Neighbourhood characteristics and urban gardens in the Toledo metropolitan area: staffing and voluntarism,food production,infrastructure, and sustainability practices

Urban gardens are important sources of sustenance for communities with limited access to food. Hence, this study focuses on food production in gardens in the Toledo metropolitan area in Northwest Ohio. We administered surveys to 150 garden managers from November 2014 to February 2015 in our attempt to better understand how neighbourhood racial composition and poverty levels are related to staffing and voluntarism, food production and distribution, the development of infrastructure, and the adoption of sustainability practices in urban gardens. The results from 30 gardens are presented in this paper. We used Geographic Information Systems to map the gardens and overlay the map with 2010 census data so that we could conduct demographic analyses of the neighbourhoods in which the gardens were located. Though the gardens were small – two acres or less – up to 46 varieties of food were grown in a single garden. Gardens also operated on small budgets. Food from the gardens was gifted or shared with friends, family, and neighbourhood residents. Gardens in predominantly minority neighbourhoods tended to have fewer institutional partners, less garden infrastructure, and had adopted fewer sustainable practices than gardens in predominantly White neighbourhoods. Nonetheless, residents of predominantly minority and high-poverty neighbourhoods participated in garden activities and influenced garden operations. Volunteering and staffing were racialised and gendered.     

Predicting deer-vehicle collisions in an urban area

Collisions with deer and other large animals are increasing, and the resulting economic costs and risks to public safety have made mitigation measures a priority for both city and wildlife managers. We created landscape models to describe and predict deer-vehicle collision (DVCs) within the City of Edmonton, Alberta. Models based on roadside characteristics revealed that DVCs occurred frequently where roadside vegetation was both denser and more diverse, and that DVCs were more likely to occur when the groomed width of roadside right-of-ways was smaller. No DVCs occurred where the width of the vegetation-free or manicured roadside buffer was greater than 40 m. Landscape-based models showed that DVCs were more likely in more heterogeneous landscapes where road densities were lower and speed limits were higher, and where non-forested vegetation such as farmland was in closer proximity to larger tracts of forest. These models can help wildlife and transportation managers to identify locations of high collision frequency for mitigation. Modifying certain landscape and roadside habitats can be an effective way to reduce deer-vehicle collisions.     

Effect of Light on Biodegradation of Estrone, 17β‐Estradiol,and 17α‐Ethinylestradiol in Stream Sediment

Biodegradation of [A‐ring ¹⁴C] Estrone (E1), 17β‐estradiol (E2), and 17α‐ethinylestradiol (EE2) to ¹⁴CO₂ was investigated under light and dark conditions in microcosms containing epilithon or sediment collected from Boulder Creek, Colorado. Mineralization of the estrogen A‐ring was observed in all sediment treatments, but not epilithon treatments. No difference in net mineralization between light and dark treatments was observed for ¹⁴C‐E2. Net mineralization of ¹⁴C‐E1 and ¹⁴C‐EE2 was enhanced in light treatments. Extents of ¹⁴CO₂ accumulation and rates of mineralization were significantly greater for E2 than E1 under dark conditions, but were comparable under light conditions. These results indicate substantial differences in the uptake and metabolism of E1 and E2 in the environment and suggest biorecalcitrance of E1 relative to E2 in light‐limited environments. The extent of ¹⁴CO₂ accumulation and rate of mineralization for EE2 in dark treatments were less than half of that observed for E2 and generally lower than for E1, consistent with previous reports of EE2 biorecalcitrance. However, ¹⁴CO₂ accumulation and rates of mineralization were comparable for EE2, E2, and E1 under light conditions. These results indicate photoactivation and/or phototransformation/photodegradation processes can substantially enhance heterotrophic biodegradation of estrogens in sunlit environments and may play an important role in estrogen transport and attenuation.     

MANGANESE CYCLE IN IMPOUNDMENT WATER

Manganese entering impounded water will undergo a cyclic transformation. Oxidation precipitates manganese from top waters. At the bottom of the lake, biological activity will render manganese soluble by both intracellular as well as extracellular activity. The study was made to demonstrate that biological reduction and transformation, organometallic complexation, together with the presence of carbon dioxide and the lowering of pH all have some effect on the solubility of manganese. Dissolved manganese may be brought back to top waters during the lake overturn and therefore deteriorates the water quality.