一株纤维素分解茵利用枳实废渣产酶条件的研究

以枳实废渣为碳源，对产纤维素酶G2茵进行固体发酵，通过测定FPA、CMC、C1酶活的大小，对其产酶条件进行优化研究。结果表明产酶最适条件为：枳实在发酵前需进行酸处理；氮源为硫酸铵；含水率为70％；初始pH为5～6；发酵周期为6天。在以上最适条件下，G2菌酶活力为FPA：31．8IU／ml，CMC：11．86IU／ml，C1：15．79IU／ml。     

玉米乳酸菌发酵营养品

本品是以玉米为主要原料，加入奶粉、少量辅料和乳酸菌发酵制成。方法包括：①选取玉米、奶粉混合均匀后，加入１０倍～１５倍纯净水，搅拌，高温灭菌，冷却至４０℃～４５℃，成玉米乳酸菌饮品培养基；②在培养基中缓慢接入菌种发酵剂，用量为培养基的８％～１５％，培养时间不少于８ｈ；③在上述饮品中加入适量的柠檬酸、糖、食用香精混合均匀后磨浆，高压均质成玉米乳酸菌发酵营养品玉米乳酸菌发酵营养品     

紫甘薯酒发酵工艺条件研究

探讨了紫甘薯酒的主要发酵工艺,采用正交试验法对紫甘薯酒的发酵条件进行了研究。结果表明,发酵液pH和酵母接种量是影响主发酵的主要因素,温度为次要因素;在最优发酵参数发酵液pH 3.8、酵母接种量3%、发酵温度18℃的条件下,可得到澄清透亮、口感较好的紫甘薯酒。     

一株产黄色素海洋细菌的筛选、鉴定及其发酵条件优化

从威海近海采得的藻类植物经过破碎和分离筛选,得到一株产生黄色素的海洋细菌,编号为SS16.通过对SS16菌株的菌落形态观察和16SrRNA基因序列测定,分析结果显示该菌株为拉塞尔佐贝尔氏菌(Zobellia russellii).为了提高SS16菌株黄色素产量,采用单因素实验设计对SS16菌株进行发酵条件优化.结果表明,当发酵条件为蛋白胨和牛肉膏作为碳、氮源,pH6.78,盐度低于1％,以占摇瓶1/5的培养量在28℃条件下发酵培养48h,海洋细菌SS16所产黄色素的量达到最大.     

江津润湿时间的温度分布研究

采用直接读取、4点统计、24点统计三种方法研究了江津试验站1998年的润湿时间的温度分布,研究表明,可以利用全年每天2、8、14、20点的4个数据统计年润湿时间的温度分布;江津地区润湿时间的温度分布特征主要为：(1)99％的年润湿时间是在5℃至30℃温度范围,每月润湿时间的温度波动一般为15℃;(2)在(0～5)℃、(5～10)℃、(10～15)℃、(15～20)℃、(20～25)℃、(25～30)℃、(30～35)℃区间内,4点统计法各区间润湿时间的百分比分别为0.34％、13．4％、24．5％、18．3％、26．0％、17．4％、0．0％。     

一株纤维素分解菌利用枳实废渣产酶条件的研究

以枳实废渣为碳源,对产纤维素酶G2菌进行固体发酵,通过测定FPA、CMC、C1酶活的大小,对其产酶条件进行优化研究.结果表明产酶最适条件为:枳实在发酵前需进行酸处理;氮源为硫酸铵;含水率为70%;初始pH为5～6;发酵周期为6天.在以上最适条件下,G2菌酶活力为FPA:31.8 IU/ml,CMC:11.86 IU/ml,C1:15.79 IU/ml.     

一株新型饲料用酶菌株的筛选及发酵条件研究

从土壤中筛选出20余株产中性植酸酶活性的菌株,对一株产中性植酸酶活性较高的绿脓假单胞菌(Phy10)进行了发酵条件优化.该菌株在pH值6.0、温度30℃、装液量100.0mL、接种量7.0mL、碳源为可溶性淀粉和氮源为NaNO2条件下产酶活性最大,优化后酶活可达到231.2U/mL,比未优化前提高了2.16倍.     

技术市场

制造生物活性颗粒饵料的方法本方法以现有蛋白质和农副产品饲料进行复配混合,其特征是在含水５０％～９０％的条件下造粒,接种可食性霉菌属并在室温条件下发酵１２ｈ～４８ｈ。本方法的特点是：原材料来源极为广泛,尤其是能选择和利用本地资源多的农副产品生产出营养全...     

碱法草浆造纸黑液厌氧生物降解研究

采用中温(34±2℃)全混合式厌氧反应器处理蒽酸和硫化烧碱草浆造纸黑液。对比厌氧发酵结合物化前或后处理两种方法进行试验,厌氧生物降解COD_(er)去除率分别为61.2～75.3％和34.9～46.2％,COD_(er)总去除率分别为80.0～87.6％和68.4～75.8％。在厌氧发酵稳态运行条件下,对发酵污泥中主要微生物类群和数量进行了研究。     

快速溶剂萃取-水浴氮吹-高效液相色谱法检测土壤16种多环芳烃

建立了快速溶剂萃取-水浴氮吹-高效液相色谱法检测土壤样品中16种多环芳烃（PAHs）的方法。分别对比了快速溶剂萃取与索式抽提、旋转蒸发浓缩与水浴氮吹浓缩、硅酸镁固相萃取柱与硅胶固相萃取柱净 化的预处理效果,经实验对比后,确认优化预处理条件为：快速溶剂萃取温度120℃,静态萃取时间16min,萃取次数两次,水浴氮吹浓缩,氮吹温度40℃,浓缩液经硅酸镁固相萃取柱净化。方法检出限和测定下限分别为0.21～0.47μg/kg和0.84～1.88μg/kg。在优化条件下,加标水平为0.5mg/kg时,目标物加标回收率为72.66％～113.90％,替代物加标回收率为69.25％,108.63％,相对标准偏差（n＝7）为0.69％～11.8％,适合实际土壤样品中PAHs的检测。     

海洋细菌产淀粉酶条件的研究

采用3,5-二硝基水杨酸显色法测定淀粉酶的酶活,研究碳源、氮源、各种理化因素等培养条件对细菌产淀粉酶的影响.结果表明,最佳碳源为麸皮,最佳氮源为蛋白胨,最适初始PH值为7.90-8.20,培养温度31℃,在装液量为100mL三角瓶中装液40mL.通过对发酵条件的优化,酶活比未优化前提高了2.71倍.     

Methane fermentation of dung from litter-free reared domestic animals — an optimisation study

Data on the quantity (27 453 tons from litter-free reared animals in Bulgaria, only) and the chemical and energy characteristics of dung produced in intensive management farms for domestic animals suggests that technologies combining biogenic elements recycling with energy utilistation and dung decontamination are expedient to be applied on these types of farms. To this effect a fermenter was designed and a mathematical model (a Chen-Hashimoto model based computer programme) was applied, as a result of which the optimum methane fermentation parameters were determined. The technological methane output (Y_v) — indicator of biogas production efficiency (output/dm³ fermentor volume) showed an optimum at temperature 55°C and period of exchange 6 days. The methane output per unit mineralised organic matter in the substrate (B) — assumed as an indicator of ecological efficiency (maximum organic matter degradation) exhibited an optimum at 33°C for 15 days period of exchange.     

发酵工业企业的清洁生产审计

本文以发酵工业企业为例,介绍了清洁生产的评价标准和审计程序,从发酵工业的特点出发提出了具体需要审计的内容,并以此说明清洁生产审计工作的重要性和需要加强的重点环节今后努力的方向。     

Cost analysis in laccase production

In this paper the cost of producing the enzyme laccase by the white-rot fungus Trametes pubescens under both submerged (SmF) and solid-state fermentation (SSF) conditions was studied. The fungus was cultured using more than 45 culture medium compositions. The cost of production was estimated by analyzing the cost of the culture medium, the cost of equipment and the operating costs. The cost of the culture medium represented, in all cases, the highest contribution to the total cost, while, the cost of equipment was significantly low, representing less than 2% of the total costs. The cultivation under SSF conditions presented a final cost 50-fold lower than the one obtained when culturing under SmF conditions at flask scale. In addition, the laccase production under SSF conditions in tray bioreactors reduced the final cost 4-fold compared to the one obtained under SSF conditions at flask scale, obtaining a final price of 0.04 cent €/U.     

UASB处理酒精废液颗粒污泥形成过程与运行效果的研究

本试验采中中温ＵＡＳＢ反应器处理酒精废液，通过启动、运行、颗粒污泥不同时期形成过程的探索，成功地培养出了颗粒污泥，取得了有机负荷高达１３－２２ｇＣＯＤ／ｌ．ｄ，ＨＲＴ１５．６－２２．３ｈｒ，ＣＯＤｃｒ去除率达８３．９－９２．２％，沼气产气率５．１－８．７１／ｌ．ｄ，ＣＨ４含量５５－７２％的良好效果。     

抗生素药生产废水处理工艺改进研究

企业抗生素废水经原有工艺处理后污染物指标偏高,且不稳定。工艺通过采用增加初沉池和二沉池、污泥回用等改进措施,COD、氨氮等排放浓度下降且稳定排放,改进效果明显。     

Recent progress in bioethanol production from lignocellulosic materials: A review

Natural energy sources like petrol and diesel are going to be diminished in the coming future which will lead to increase in the prices and demands of fossil fuels. Therefore, it is important to find a sustainable alternate of fossil fuels. Bioethanol is one of the alternatives, which is produced from different feedstocks including sugar-based, starch-based and lignocellulose-based materials through fermentation. Since sugar-based (sugar cane and sugar beet) and starch-based (corn) materials are sources of staple food, therefore, research on lignocellulosic materials for bioethanol production is a subject of recent studies. Ethanol production from lignocellulosic materials involves different steps, such as pretreatment, hydrolysis, followed by fermentation process and finally ethanol purification. In this review, we have summarized the recent progresses in bioethanol production and processing from lignocellulosic materials.     

城市生活垃圾发酵制肥

本文以国内外的工程实践为基础,从技术和经济角度介绍城市生活垃圾中的可腐有机物发酵制肥的基本原理、好氧和厌氧发酵技术的工艺特点、适应条件、建设投资、资源回收和环境影响等。建议在我国经济发达的城市推广应用厌氧发酵制肥技术。     

Biohydrogen production by Clostridium butyricum and Rhodopseudomonas palustris in Co-cultures

This study evaluated biohydrogen production by co-culture of Clostridium butyricum and Rhodopseudomonas palustris. C. butyricum and R. palustris were grown separately and together as batch cultures. Hydrogen production, growth, NH₄-N, total volatile fatty acid production, and sucrose degradation were monitored. The hydrogen production of the co-culture produced 562 ml, R. palustris 426 ml, and C. butyricum 333 ml. The co-culture produced 2.16 mol H₂/mol sucrose, C. butyricum and R. palustris produced 1.77 and 1.64 mol H₂/mol sucrose, respectively. The co-culture was more efficient in the hydrogen production. Therefore, the co-culture is a good method for biohydrogen fermentation.