共查询到19条相似文献,搜索用时 62 毫秒
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采用环氧苯乙烯和血红蛋白中羧酸基和巯基位的加合物同时测定的方法,对大鼠血红蛋白SO加的进行了体外实 腹腔注射环氧苯乙烯和苯乙烯的体内实验。结果表明在血红蛋白中所分析的3种加合物(SG,1-PE,2-PE)均随着SO剂量而增加。 相似文献
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共生细菌SB_1接种在0.3%聚乙烯醇(PVA)培养基中,30℃摇床振荡培养(500mL三角瓶)或发酵罐(1000L)通气培养时,PVA氧化酶产生的高峰在48h或40h.离心收集的上清液采用40%-50%饱和度的硫酸铵分级沉淀DEAE-Sephadex离子交换柱和Cibacron蓝-Sepharose4B亲和柱层析等纯化步骤,PVA氧化酶收得率30%,比活力提高31倍,该酶经凝胶电泳后,用硝基四氮唑蓝进行活性染色,呈现四条谱带,PVA氧化酶与PVA作用,可释放H_2O_2,同时伴随着粘度降低,PVA氧化酶反应的最适pH为7.0,最适温度为40℃,该酶置-5℃贮存较稳定,贮存500天,其活力仍保留近50%,在所测试的金属离子和化学试剂中,Pb ̄(2+)和水杨酸可使PVA氧化酶活力完全丧失。而Ba ̄(2+)与NaN_3则对酶活力有较强的激活作用。 相似文献
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用苯及其生物代谢物酚、醌处理大鼠各器官中DNA加合物的形成和分布 总被引:1,自引:0,他引:1
用苯及其在生物体内代谢物酚,醌处理大鼠,24h后取其肝、肾、肺、脾诸组织,用~(32)P后标记方法测4种器官中DNA加合物含量,其结果是:在上述各器官中均发现有DNA加合物的形成,4种器官中总加合物量值对苯、酚、醌依次为9.18─89.13、20.9─243.8、53.1─308.9加合物/10~8正常核酸,3种系列化合物对大鼠4种器官中DNA加合物形成能力依次为醌>酚>苯。而大鼠中4种器官对同一化合物的加合物形成能力依次为肝>肾>肺>脾。这种结果较好地反映出3种系列化合物的代谢过程和生态毒理效应的差异性。 相似文献
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Analysisofstyreneoxide-hemoglobinadductsatdiferentbindingsitesJinZuliang,LiuShufenTheResearchCentreforEcoEnvironmentalScien... 相似文献
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To explore potentially selective growth conditions for fetal cells in cultures from the blood of pregnant women, we investigated if fetal and adult erythroid progenitors with different hemoglobin expression programs are differentially responsive to erythropoietin (EPO). Co-cultures of clonogenic cells from 12-week fetal and adult peripheral blood were established, and the development of erythropoietic cells was monitored using flow cytometric profiles of correlated cellular contents of fetal and adult hemoglobin (HbF and HbA, respectively). Adult nucleated red cells were classified as F+A−, F+A+ or F−A+. All fetal cells were F+A−. The population of F+A− cells was flow-sorted and fetal cells were identified by fluorescence in situ hybridization (FISH) using chromosome-specific probes. Delayed EPO addition revealed that all types of erythroid cells entered the EPO-dependent phase with similar kinetics, beginning at about Day 4. The data suggest that fetal and adult erythroid stem/progenitor cells have the same initial maturation kinetics in culture independent of their hemoglobin chain expression program. Fetal and adult cells with different hemoglobin profiles also showed similar EPO dose–response curves, determined for different intervals during the first 2 weeks of culture. Thus, the kinetics of entry into the phase of EPO dependence, as well as the sensitivity to EPO at various stages of development, are essentially the same for erythropoietic progenitor cells derived from adult and early fetal blood, which rules out the possibility of using the timing or concentration of EPO for the selective growth of fetal cells from the blood of pregnant women. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
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Goonnapa Fucharoen Warunee Tungwiwat Thawalwong Ratanasiri Kanokwan Sanchaisuriya Supan Fucharoen 《黑龙江环境通报》2003,23(5):393-396
In order to provide a noninvasive prenatal diagnosis of the hemoglobin E (Hb E) related disorder, we have evaluated the possibility of identifying the fetal βE-globin gene in maternal plasma. The analysis was performed during 8 to 18 weeks of gestation using DNA extracted from 200 µL of plasma from pregnant women whose husbands carried Hb E. The βE-globin mutation in maternal plasma was detected by a nested PCR amplification followed by the Mnl I restriction analysis. The result was compared with that of routine analysis of the CVS specimens. Among the five pregnant women examined, the fetal βE-globin gene was identified in maternal plasma in three of them and the result was completely concordant with the conventional CVS analysis. This simple noninvasive prenatal detection of the fetal βE-globin gene should prove useful in a prevention and control program of Hb E/β-thalassemia in countries where the βE-globin gene is prevalent. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
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生物电催化方法处理三氯乙酸的研究 总被引:5,自引:2,他引:3
利用吸附法将血红蛋白(Hb)固定在碳纳米管修饰电极表面,研究了Hb在碳纳米管修饰电极的直接电化学行为.固载Hb碳纳米管修饰电极在pH=7.0的PBS缓冲溶液中于-0.300V(vsSCE)处有一对相当可逆的循环伏安还原氧化峰,为Hb血红素辅基Fe(Ⅲ)/Fe(Ⅱ)电对的特征峰.利用循环伏安法和恒电位电解法研究了固载Hb的碳纳米管修饰电极对有机氯模型污染物三氯乙酸的电催化还原脱氯,并通过鉴定中间产物探讨了其催化还原机理.结果表明,固载Hb的碳纳米管修饰电极对三氯乙酸的还原具有很高的催化活性,三氯乙酸是按照三氯乙酸→二氯乙酸→一氯乙酸→乙酸的途径分布还原脱氯.将固载Hb的碳纳米管修饰电极组装成三维填充床电解反应器,进行了三氯乙酸连续流动电解还原的初步研究.-0.6V(vs.SCE)电解180min,三氯乙酸去除率为40.13%. 相似文献
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Low levels (ng/g) of musk ketone (MK),used as a fragrance additive in the formulation of personal care products,are frequently detected in the water and other environment.Thus,aquatic organisms can be continuously exposed to MK.In this study,kinetics and dose-response assessments of 2-amino-MK (AMK) metabolite,bound to cysteine-hemoglobin (Hb) in rainbow trout,formed by enzymatic nitro-reduction of MK have been demonstrated.Trout were exposed to a single exposure of 0.010,0.030,0.10,and 0.30 mg MK/g fish... 相似文献
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Effects of two sludge application on fractionation and phytotoxicity of zinc and copper in the soil 总被引:1,自引:0,他引:1
HE Miao-miao TIAN Guang-ming LIANG Xing-qiang YU Yi-tong WU Jian-yang ZHOU Gen-di 《环境科学学报(英文版)》2007,19(12)
The potential harm of heavy metals is a primary concern in application of sludge to the agricultural land. A pot experiment was conducted to evaluate the effect of two sludges on fractionation of Zn and Cu in soil and their phytotoxicity to pakchoi. The loamy soil was mixed with 0%, 20%, 40%, 60% and 80% (by weight) of digested sewage sludge (SS) and composted sludge (SC). The additions of both sludges caused a significant raise in all fractions, resulting in that exchangeable (EXCH) and organic bound (OM) became predominance of Zn and organic bound Cu occupied the largest portion. There was more available amount of Zn and Cu in SS treatments than SC treatments. During the pot experiment, the concentration of Zn in EXCH, carbonate (CAR) and OM and Cu in EXCH and OM fractions decreased in all treatments, so their bioavailability reduced. Germination rate and plant biomass decreased when the addition rate was high and the best yield appeared in 20% mixtures at the harvest of pakchoi. The two sludges increased tissue contents of Zn and Cu especially in the SS treatments. Zn in pakchoi was not only in relationship to ΔEXCH and ΔCAR forms but also in ΔOM forms in the sludge-soil mixtures. Tissue content of Cu in pakchoi grown on SC-soils could not be predicted by ΔEXCH. These correlation rates between Zn and Cu accumulation in pakchoi and variation of different fractions increased with time, which might indicate that sludges represented stronger impacts on the plant in long-term land application. 相似文献