Chorionic villus sampling for prenatal diagnosis in wales using DNA probes—5 years' experience

Chorionic villi were sampled from 125 women who requested prenatal diagnosis, either for genetic disorders or because of advanced maternal age. Of these, 105 samples were obtained by the transcervical route and 20 were obtained by the transabdominal approach. The sampling success rate was 97 per cent (122/125). The mean maternal age of the patients was 31 years (range 17–44) and the mean gestational age at which the chorionic villus sampling was performed was 10 weeks (range 7–13 weeks). Seventy-four of these diagnoses involved the use of DNA markers. The minimal size of the sample used for DNA diagnosis was 5 mg. Maternal contamination was detected in two samples. A diagnosis was provided on all but two samples. The fetal loss rate was high initially but fell to 1·9 per cent in 1988.     

Correlations between in situ denitrification activity and nir-gene abundances in pristine and impacted prairie streams

Denitrification is a process that reduces nitrogen levels in headwaters and other streams. We compared nirS and nirK abundances with the absolute rate of denitrification, the longitudinal coefficient of denitrification (i.e., K_den, which represents optimal denitrification rates at given environmental conditions), and water quality in seven prairie streams to determine if nir-gene abundances explain denitrification activity. Previous work showed that absolute rates of denitrification correlate with nitrate levels; however, no correlation has been found for denitrification efficiency, which we hypothesise might be related to gene abundances. Water-column nitrate and soluble-reactive phosphorus levels significantly correlated with absolute rates of denitrification, but nir-gene abundances did not. However, nirS and nirK abundances significantly correlated with K_den, as well as phosphorus, although no correlation was found between K_den and nitrate. These data confirm that absolute denitrification rates are controlled by nitrate load, but intrinsic denitrification efficiency is linked to nirS and nirK gene abundances.     

Energetics of mixing for the filling box and the emptying-filling box

The mixing efficiency of a plume in a filling box and an emptying-filling box is calculated for both transient and steady states. The mixing efficiency of a plume in a filling box in an asymptotic state is 1/2, independent of the details of this state or how the plume is modelled. The mixing efficiency of a plume in an emptying-filling box in steady state is \(1 - \xi \), where \(\xi = h/H\), the depth of the ambient layer h normalised by the height of the box H. A deeper mixed layer therefore corresponds to a higher mixing efficiency. These results shed light on the interpretation of mixing efficiencies of open and closed systems.

     

Trends in anecdotal fox sightings in Tasmania accounted for by psychological factors

There has been little evaluation of anecdotal sightings as a means to confirm new incursions of invasive species. This paper explores the potential for equivocal information communicated by the media to account for patterns of anecdotal reports. In 2001, it was widely reported that red foxes (Vulpes vulpes) had been deliberately released in the island state of Tasmania (Australia), although this claim was later revealed to be baseless. Regardless, by 2013 a total of 3153 anecdotal fox sightings had been reported by members of the public, which implied their distribution was wide. For each month in 2001–2003, we defined a monthly media index (MMI) of fox‐related media coverage, an index of their relative seasonal abundance (abundance), and a factor denoting claims of fox evidence (claimed evidence) regardless of its evidentiary quality. We fitted a generalized linear model with Poisson error for monthly totals of anecdotal sightings with factors of year and claimed evidence and covariates of MMI, abundance, and hours of darkness. The collective effect of psychological factors (MMI, claimed evidence, and year) relative to biophysical factors (photoperiod and abundance) was highly significant (χ² = 122.1, df = 6, p < 0.0001), whereas anticipated changes in abundance had no significant influence on reported sightings (p = 0.15). An annual index of fox media from 2001 to 2010 was strongly associated with the yearly tally of anecdotal sightings (p = 0.018). The odds ratio of sightings ranked as reliable by the fox eradication program in any year decreased exponentially at a rate of 0.00643 as the total number of sightings increased (p < 0.0001) and was indicative of an observer‐expectancy bias. Our results suggest anecdotal sightings are highly susceptible to cognitive biases and when used to qualify and quantify species presence can contribute to flawed risk assessments.     

Quantitative assessment of medical waste generation in the capital city of Bangladesh

There is a concern that mismanagement of medical waste in developing countries may be a significant risk factor for disease transmission. Quantitative estimation of medical waste generation is needed to estimate the potential risk and as a basis for any waste management plan. Dhaka City, the capital of Bangladesh, is an example of a major city in a developing country where there has been no rigorous estimation of medical waste generation based upon a thorough scientific study. These estimates were obtained by stringent weighing of waste in a carefully chosen, representative, sample of HCEs, including non-residential diagnostic centres. This study used a statistically designed sampling of waste generation in a broad range of Health Care Establishments (HCEs) to indicate that the amount of waste produced in Dhaka can be estimated to be 37 ± 5 ton per day. The proportion of this waste that would be classified as hazardous waste by World Health Organisation (WHO) guidelines was found to be approximately 21%. The amount of waste, and the proportion of hazardous waste, was found to vary significantly with the size and type of HCE.     

Beyond sustainability indicators

Sustainability indicators are an increasingly popular tool for the identification of policies and monitoring of progress towards sustainable development. The need for indicators is clearly set out in Agenda 21 and has been taken up by the Commission for Sustainable Development. Devising alternative measures of progress to gross national product has been the subject of much research in the past few years. There are many local sustainability indicator initiatives now under way, co-ordinated by local authorities and involving local communities. However useful these exercises have been (not least to those engaged in them) there is little evidence, so far, that sustainability indicators are leading to substantial shifts in policy at national or local level. Evidence points, in fact, to substantial barriers to progress in several key areas: for example, the necessity for the greater integration of environmental, social and economic policy, the tackling of inequality and poverty and the encouragement of greater public participation in action on sustainable development. In order for indicators to make any progress in surmounting these barriers there is a need to address issues of trust and to examine existing institutional structures and practices. In parallel with the development of indicators, national, and particularly local, government will need to experiment with new and creative techniques for community participation in decision making, engage in dialogue with new cultural networks and implement practical initiatives to improve the quality of life in particular communities.     

What do significance tests really tell us about the environment?

Routine application of significance tests does not extract the maximum information from environmental data and can lead to misleading conclusions. Reasons leading to this are: a significant result can often be reached merely by collecting enough samples; a statistically significant result is not necessarily practically significant; and reports of the presence or absence of statistically significant differences for multiple tests are not comparable unless identical sample sizes are used. These problems are demonstrated by application to pH data for grazed and retired fields, and by discussion of significance tests used in recent US regulations for groundwater quality. The advantages of equivalence tests, where the tester must state the difference of practical difference, are discussed and applied to the field pH problem. We recommend that environmental managers and scientists pay more attention to statistical power and decide on what is a practical difference. Confidence intervals for the size of the differences, accompanied where necessary by equivalence tests, are the preferred means of addressing the question: “is there a difference of practical significance?”     

A national critical loads framework for atmospheric deposition effects assessment: II. Defining assessment end points,indicators, and functional subregions

The United States Environmental Protection Agency, with support from the US Department of Energy and the National Oceanographic and Atmospheric Administration, has been evaluating the feasibility of an effects-based (critical loads) approach to atmospheric pollutant regulation and abatement. The rationale used to develop three of the six steps in a flexible assessment framework (Strickland and others, 1992) is presented along with a discussion of a variety of implementation approaches and their ramifications. The rationale proposes that it is necessary to provide an explicit statement of the condition of the resource that is considered valuable (assessment end point) because: (1) individual ecosystem components may be more or less sensitive to deposition, (2) it is necessary to select indicators of ecosystem condition that can be objectively measured and that reflect changes in the quality of the assessment end point, and (3) acceptable status (i.e., value of indicator and quality of assessment end point at critical load) must be defined. The rationale also stresses the importance of defining the assessment regions and subregions to improve the analysis and understanding of the indicator response to deposition. Subregional definition can be based on a variety of criteria, including informed judgment or quantitative procedures. It also depends on the geographic scale at which exposure and effects models are accurate and on data availability, resolution, and quality. The research described in this article has been funded by the US Environmental Protection Agency. This document has been prepared at the EPA Environmental Research Laboratory in Corvallis, Oregon, through contract #68-C8-0006 with ManTech Environmental Technology, Inc., and Interagency Agreement #1824-B014-A7 with the US Department of Energy and at Oak Ridge National Laboratory managed by Martin Marietta Energy Systems, Inc., under Contract DE-AC05-84OR21400 with the US Department of Energy. Environmental Sciences Division Publication No. 3903. It has been subjected to the agency’s peer and administrative review and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.     

Place and personal identity in old age: Observations from Appalachia

This paper explores the phenomenon of attachment to place in old age. Three complementary dimensions of attachment—physical, social and autobiographical insideness—are identified from a three year in-depth study of elderly residents of an Appalachian community. It is argued that autobiographical insideness may be particularly important to old people in sustaining a sense of personal identity and may be adaptive in old age. The paper develops the hypothesis that the increasing mobility of current elderly generations is resulting in changing manifestations of attachment to place. Whereas the old-old (persons over 75 years of age) remain strongly attached to their proximate physical environment, the young-old (those under 75 years of age) appear to be developing identifications with places that involve greater emphasis upon vicarious involvement in displaced settings.