The advent of automated vehicles is already taking place and will significantly disrupt the motor insurance industry. The shift from the human driver to the system as the driver cannot be reflected in the current insurance risk assessment. This call for an amendment of the insurance underwriting was discussed with German experts from both the primary insurance and reinsurance sector with their professional background on motor insurance. Based on the findings, we propose an alternative method to underwrite automated vehicles of level 4 & 5 using an enhanced telematics approach which considers new risk categories such as systems used and the transformed role of the driver as the general user of the automated vehicle.
There have been a number of studies investigating metal uptake in plants on contaminated landfill sites, but little on their role as biomarkers to identify metal mobility for continuous monitoring purposes. Vegetation can be used as a biomonitor of site pollution, by identifying the mobilisation of heavy metals and by providing an understanding of their bioavailability. Plants selected were the common nettle (Uritica Dioica), bramble (Rubus Fruticosa) and sycamore (Acer Pseudoplatanus). A study of the soil fractionation was made to investigate the soil properties that are likely to influence metal mobility and a correlation exercise was undertaken to investigate if variations in concentration of metals in vegetation can reflect variations in concentration of the metals in soil. The soil was digested using aqua regia in a microwave closed vessel. The vegetation was digested using both microwave and a hydrogen peroxide-nitric acid mixture, refluxed on a heating block and a comparison made. The certified reference materials (CRMs) used were Standard Reference Material (SRM) 1547, peach leaves for vegetation (NIST) and for soil CRM 143R, sewage sludge-amended soil (BCR). The relative standard deviations (RSDs) were 2-6% for the analyses. Our findings show evidence of phytoextraction by some plants, (especially bramble and nettle), with certain plants, (sycamore) exhibiting signs of phytostabilisation. The evidence suggests that there is a degree of selectivity in metal uptake and partitioning within the plant compartments. It was also possible to correlate mobility phases of certain metals (Pb, Cu and Zn) using the soil and plant record. Zn and Cu exhibited the greatest potential to migrate from the roots to the leaves, with Pb found principally in the roots of ground vegetation. Our results suggest that analysis of bramble leaves, nettle leaves and roots can be used to monitor the mobility of Pb in the soil with nettle, bramble and sycamore leaves to monitor Cu and Zn. 相似文献
In July 2002, a multi-sensor campaign was conducted in southern France to investigate the hypothesized connection between
gravity waves and optical turbulence. A generalized scidar (GS) was mounted on the 1.93 m diameter telescope at l’Observatoire
de Haute-Provence (OHP). The GS provides continuous profiles of optical turbulence with 300 m vertical resolution from telescope
altitude up to 25 km. Thermosondes, which provide in situ measurement of optical turbulence by measuring temperature variance,
were launched at the OHP site and at a site approximately 20 km west-northwest of OHP. Gravity wave activity was deduced from
temperature and wind velocity measured by radiosondes, which are part of the thermosonde system. In this paper, gravity waves
were analyzed using techniques for simple two-dimensional mountain waves, with only fair results. Mesoscale models were run
at moderately high resolution for the period. The forecasts were analyzed for wave activity, and a post processor model was
used to diagnose the optical turbulence. Mountain waves were evident in the forecast, but quantitative comparison showed the
forecast to be inadequate in predicting wave strength. The forecast optical turbulence was in fair agreement with measurements
with notable exceptions.
This paper is declared a work of the U.S. Government and is not subject to copyright protection in the United States. The
U.S. Government’s right to retain a non-exclusive, royalty-free license in and to any copyright is acknowledged.
Presented at the Turbulence and Waves in Stably Stratified Atmospheric Shear Flows: Measurements, High Resolution Simulations
and Numerical Prediction Challenges Conference, 13–15 September 2004, Lighthill Institute of Mathematical Sciences, University
College London, London, UK. 相似文献
Bottom-feeding fish such as flounder and killifish have been widely used in monitoring hepatic monooxygenase induction in polluted water bodies. While channel catfish are often utilized in tissue monitoring of fresh and estuarine water bodies, few data are available on their use in environmental monitoring of hepatic monooxygenase activity. In this project, the presence of CYP1A protein was verified in channel catfish through recognition by Mab 1-12-3, an antibody which recognizes the CYP1A homologue in a variety of teleost species. CYP1A protein levels and 7-ethoxyresorufin-o-deethylase (EROD) activity in laboratory control and benzo-a-pyrene (BaP)-challenged channel catfish were compared to those in feral channel catfish from Back River, an urban estuarine tributary to Chesapeake Bay. Though more variable, mean CYP1A protein levels in the field-collected fish were similar to those of the BaP-induced laboratory fish. However, EROD activity of the Back River fish was less than one half that observed in the BaP-induced laboratory fish. When normalized to CYP1A protein levels, EROD activity was slightly lower in the Back River fish than either the laboratory control or BaP-treated fish. This finding may indicate possible inhibition or inactivation of the CYP1A protein in the feral fish. 相似文献
Dozens of schemes have been developed for assessing the hazards and implicit risks posed by waste disposal sites in the United States. Effective hazard-assessment processes guide decision makers, policy makers, and the public toward more appropriate risk management solutions. Just as in quantitative risk assessment, however, the utility of a hazard assessment scheme depends upon the applicability and totality of the parameters, as well as upon the validity of the assumptions and logic underlying that process. Examination of the range of hazard assessment methods that have been employed reveals some common limitations and a universe of applicable parameters. Understanding the scope of United States efforts is vital to the effective transfer of hazard assessment technology to waste site stakeholders in other industrialized countries. A useful set of modeling criteria is provided as a foundation for future application of the hazard assessment concept at the thousands of waste sites worldwide where hazards are yet to be investigated. 相似文献
There is a widely acknowledged need for a single composite index that provides a comprehensive picture of the societal impact of disasters. A composite index combines and logically organizes important information policy‐makers need to allocate resources for the recovery from natural disasters; it can also inform hazard mitigation strategies. This paper develops a Disaster Impact Index (DII) to gauge the societal impact of disasters on the basis of the changes in individuals’ capabilities. The DII can be interpreted as the disaster impact per capita. Capabilities are dimensions of individual well‐being and refer to the genuine opportunities individuals have to achieve valuable states and activities (such as being adequately nourished or being mobile). After discussing the steps required to construct the DII, this article computes and compares the DIIs for two earthquakes of similar magnitude in two societies at different levels of development and of two disasters (earthquake and wind storm) in the same society. 相似文献
Herein we present a campaign dedicated to the detection and the characterization of Gravity Waves (GW) in the Earth’s atmosphere
in relation to the generation of Optical Turbulence (OT). The observations took place in France from 17 to 24 July 2002 at
the Haute Provence Observatory (OHP) and simultaneously at the Sirene Observatory, some 20 km apart. From both sites, several
balloons were launched that measured the classical PTU-Wind profiles and additionally the structure constant of the temperature
field vertical profiles. A Generalized Scidar (GS) technique was implemented at the 1.93 m-diameter OHP telescope, providing profiles every minute. From our observations, a significant amount of GW activity was observed at both sites, but without
clear evidence of correlation between the two sites. It seems from our observations that a wide spectrum of GW is present
at a given altitude and that this could result in a lack of correlation between observations made from two sites 20 km apart.
Most GW are non-stationary with long horizontal wavelengths (λ ∼ 100–200 km), kilometric vertical wavelengths (λ ~ 0.5–2 km)
and long intrinsic period (T ~ 2–15 h). They belong in the category of “hydrostatic rotating or non-rotating waves”. Layers of optical turbulence detected
by balloons and the Scidar technique correlate well with regions of GW activity.
The U.S. Government’s right to retain a non-exclusive, royalty-free license in and to any copyright is acknowledged. 相似文献
In response to increasing concerns regarding the potential of chemicals to interact with the endocrine system of humans and wildlife, various national and international programs have been initiated with the aim to develop new guidelines for the screening and testing of these chemicals in vertebrates. Here, we report on the validation of an in vitro assay, the H295R steroidogenesis assay, to detect chemicals with the potential to inhibit or induce the production of the sex steroid hormones testosterone (T) and 17??-estradiol (E2) in preparation for the development of an Organization for Economic Cooperation and Development (OECD) test guideline.
Methods
A previously optimized and pre-validated protocol was used to assess the potential of 28 chemicals of diverse structures and properties to validate the H295R steroidogenesis assay. These chemicals are comprised of known endocrine-active chemicals and ??negative?? chemicals that were not expected to have effects on the targeted endpoints, as well as a number of test chemicals with unknown modes of action at the level of the steroidogenic pathway. A total of seven laboratories from seven countries participated in this effort. In addition to effects on hormone production, confounding factors, such as cell viability and possible direct interference of test substances with antibody-based hormone detection assays, were assessed. Prior to and during the conduct of exposure experiments, each laboratory had to demonstrate that they were able to conduct the assay within the margin of predefined performance criteria.
Results
With a few exceptions, all laboratories met the key quality performance parameters, and only 2% and 7% of all experiments for T and E2, respectively, were excluded due to exceedance of these parameters. Of the 28 chemicals analyzed, 13 and 14 tested affected production of T and E2, respectively, while 11 and 8 did not result in significant effects on T and E2 production, respectively. Four and six chemicals produced ambiguous results for effects on T and E2 production, respectively. However, four of these cases each for T and E2 were associated with only one laboratory after a personnel change occurred. Significant interference of test chemicals with some of the antibody-based hormone detection systems occurred for four chemicals. Only one of these chemicals, however, significantly affected the ability of the detection system to categorize the chemical as affecting E2 or T production.
Discussion and conclusions
With one exception, the H295R steroidogenesis assay protocol successfully identified the majority of chemicals with known and unknown modes of interaction as inducers or inhibitors of T and E2 production. Thus it can be considered a reliable screen for chemicals that can alter the production of sex steroid hormones. One of the remaining limitations associated with the H295R steroidogenesis assay protocol is the relatively small basal production of E2 and its effect on quantifying the decreased production of this hormone with regard to the identification of weak inhibitors. An initial comparison of the data produced in this study with those from in vivo studies from the literature demonstrated the potential of the H295R steroidogenesis assay to identify chemicals affecting hormone homeostasis in whole organisms. Particularly promising was the lack of any false negatives during the validation and the very low number of false positives (1 out of 28 chemicals for each T and E2).
Perspectives
Based on the results obtained during this validation study and the accordingly revised test protocols, an OECD draft test guideline was developed and submitted to the OECD working group of the national coordinators of the test guidelines program (WNT) for comments in December 2009. 相似文献