我国安全生产法制对策研究

分析了国内外安全生产法制现状，总结提出了我国存在的职业安全与健康分离、没有系统和规划、适用和衔接性差等特点和问题。阐述了安全生产法规体系建设的指导思想、基本原则以及基本框架，并提出了定期制定安全生产法规规划、梳理与评估法规体系实施效果、制定统一的劳动安全与健康法、编制安全生产计划、健全职业健康配套规定、保险与安全生产互动、以明确综合监管和专项监管以及安全生产规划、完善隐患治理、安全使用机械、健全企业自主管理体制等为重点对《安全生产法》进行修订的对策。     

Extended mutualism between termites and gut microbes: nutritional symbionts contribute to nest hygiene

All higher eukaryotes have established symbiotic relationships with diverse microorganisms. One of the most well-characterized symbiotic systems is that of termites and their intestinal microorganisms, which digest cellulose. Recently, diverse types of symbioses between gut microbes and host organisms including humans have received growing attention for various features of their complex interactions beyond nutrition. In termites, researchers are beginning to explore such function of gut symbionts, but only the contribution to internal immunity against entomopathogen is known in a few species. Here, we report that gut symbionts of the dampwood termite Zootermopsis nevadensis protect nests from the spread of the commensal bacterium Serratia marcescens, which has pathogenic potential. Defaunated termites dispersed S. marcescens in the surrounding environment by feeding on the bacteria, which then survived passage through their alimentary tracts, while non-defaunated termites did not. Loss of gut symbionts caused a significant reduction in intestinal acetate, which is an important carbon source for termites. Culture experiments showed that acetate had significant inhibitory effects on S. marcescens at a concentration as low as 12 mM, which indicated that the intestinal acetate of non-defaunated termites (40–130 mM) was capable of suppressing this bacterium. These results suggest that digestive derivatives produced by intestinal symbionts play an essential role in nest hygiene in addition to their nutritional function for termites. Our study provides a better understanding of the multifunctionality of symbiotic relationships in diverse organisms beyond nutrition.     

Spatial Assessment of the Alder Tree in Kushiro Mire, Japan using Remotely Sensed Imagery – Effects of the Surrounding Land Use on Kushiro Mire

The relationship between alder (Alnus japonica) distribution and surrounding land use in Kushiro Mire was spatially assessed using remotely sensed imagery. From the result, it was found out that the expanding area of alder trees in Kushiro Mire was affected by the agricultural land area in the upper course of the river basin and flooding in the lower course of the river. The soil sediments flowing into the Kushiro Mire from the agricultural land resulted in heavy sedimentation that favors the growth of alder trees. Consequently, the number and density of alder trees has increased. The future distribution of alder trees was predicted based on the mechanism of expansion of the alder-tree area in Kushiro Mire, and it was found that large vegetation areas in Kushiro Mire will be changed to areas with alder trees.     

抚顺城区大气悬浮颗粒物污染与呼吸系统疾病死亡率相关性的定量分析

为了定量分析大气总悬浮颗粒物(TSP)对居民呼吸系统疾病死亡率的影响,采用单因素泊松(Poisson)回归分析法,研究了辽宁省抚顺市城区TSP浓度变化与市区1999～2003年呼吸系统疾病死亡率的相关关系.结果发现,在消除了时间、气象等因素影响后,滞后4日TSP浓度每增加50μg·m-3,呼吸系统疾病总人群组死亡率相对危险度(OR)增加1.02195(95%CI=1.00195～1.04245),即滞后4日TSP浓度变化造成总人群组呼吸系统疾病死亡率明显增加的超额死亡率为2.195%.抚顺城区大气TSP污染可能是市区居民呼吸系统疾病死亡率增加的原因之一.     

Biodegradation of synthetic surfactants by river microorganisms

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Genetic influence on caste determination underlying the asexual queen succession system in a termite

The question of how reproductives and sterile workers differentiate within eusocial groups has long been a core issue in the study of social insects. Recent studies have shown that not only environmental factors but also genetic factors affect caste differentiation. In the termite Reticulitermes speratus, queens produce their replacements (neotenics) asexually but use normal sexual reproduction to produce other colony members. Here, we demonstrate a genetic influence on caste determination underlying the asexual queen succession system in this termite species. Thelytoky in termites is accomplished by automixis with terminal fusion, yielding almost completely homozygous offspring; thus, parthenogenetically and sexually produced offspring profoundly differ in heterozygosity. An analysis of the relationship between the reproductive dominance of female neotenics obtained from experimentally orphaned colonies and their genotypes at five microsatellite loci showed that homozygosity at two loci influenced the developmental priority and/or reproductive quality of neotenics. These results suggest the existence of a multi-locus system affecting the queen fecundity and explain why parthenogens have genetic priority to become neotenics in this termite species.     

Ultraweak photon emission from herbivory-injured maize plants

Following perception of herbivory or infection, plants exhibit a wide range of inducible responses. In this study, we found ultraweak photon emissions from maize leaves damaged by Helicoverpa armigera (Noctuidae). Interestingly, mechanically damaged maize leaves treated with caterpillar regurgitants emitted the same intensity and pattern of photon emissions as those from maize leaves damaged by caterpillars. Furthermore, two-dimensional imaging of the leaf section treated with the oral secretions clearly shows that photon emissions were observed specifically at the lip of the wound exposed to the secretions. These results suggest that the direct interaction between maize leaf cells and chemicals contained in caterpillar regurgitants triggers these photon emissions.     

Removal of pentachlorophenol from contaminated soil by catalytic oxidation with iron(III)-porphyrin complexes and potassium monopersulfate

Pentachlorophenol (PCP) in contaminated soil was removed by treatment with aqueous solutions of iron(III)-porphyrin complexes as catalysts and potassium monopersulfate (KHSO₅) as the oxygen donor. The contaminated soils were artificially prepared by spiking PCP to the kaolin and ando soils. Three types of iron(III)-porphyrin complexes, tetra(?p-sulfophenyl) porphineiron(III) (Fe(III)-TPPS), tetra(N-methyl-4-pyridil)porphineiron(III) (Fe(III)-TMPyP) and heme, were examined, and Fe(III)-TPPS was found to be the most effective for removing PCP. Although the sequential addition of KHSO₅ was examined, in an attempt to improve the efficiency of PCP removal, it was not effective. In a preliminary test of various aqueous solutions, the addition of humic acid (HA), with a lower degree of humification, led to a significant enhancement in PCP removal. When HA was added to the soil system, the percentages of PCP removal were increased by up to 10% compared to the absence of HA. Therefore, the addition of HA to the catalytic system was useful in enhancing PCP removal from contaminated soil.     

Industrial waste management simulation game and its educational effect

The purposes of this study were to develop the Industrial Waste Game and to examine the validity of this game as a tool for environmental education. The aim of this game is to enable players to understand the social dilemma between individual interest of hazardous dumping and the social cost of purifying pollution, and to find a solution to the social dilemma by providing the monitoring and sanction system for illegal dumping or other efficient systems. The game was played by 213 undergraduate students who were divided into 40 groups of 5 to 6 people. One of 4 combinations of monitoring and penalty conditions was assigned to each group. The players were asked to answer questionnaires concerning their interests and understanding of the industrial waste issue before and after the game to evaluate educational effects. The results indicated that the players increased their awareness of industrial waste problems and came to understand that these problems were caused not by psychological factors such as immorality of the illegal dumper but mainly by social structural factors like the social dilemma. Through playing the game and participating in post-game discussion, players were able to obtain interesting experience and gain motivation to learn more.     

餐厨垃圾发酵制备生物燃料丁醇的研究

餐厨垃圾含丰富有机质,是潜在的廉价优质生物质资源;丁醇是继乙醇后的一种极具潜力的新型生物燃料。探索了以餐厨垃圾糖化液为原料,通过微生物发酵制取生物燃料丁醇。首先从实验室现有5株产丁醇梭菌中,优选出Clostridium beijerinckii NCIMB 8052作为丁醇生产菌,并考察在未添加任何营养物质,且不调节pH的条件下(简称非调控状态),以糖化液作为底物进行丁醇发酵的可行性。结果表明:非调控状态发酵的丁醇产量仅为5.96 g/L,并产生"酸崩"抑制;添加0.3%(w/V)Ca CO_3提高了糖化液pH缓冲能力,可解决"酸崩"问题,并将丁醇产量、总溶剂产量及丁醇生产速率分别提高了57.8%、53.1%和90.6%。这证明餐厨垃圾做为原料用于燃料丁醇生产是完全可行的。