The rhizosphere microbiome plays critical roles in plant growth and is an important interface for resource exchange between plants and the soil environment. Crops at various growing stages, especially the seedling stage, have strong shaping effects on the rhizosphere microbial community, and such community reconstruction will positively feed back to the plant growth. In the present study, we analyzed the variations of bacterial and fungal communities in the rhizosphere of four crop species: rice, soybean, maize, and wheat during successive cultivations (three repeats for the seedling stages) using 16S rRNA gene and internal transcribed spacer (ITS) high-throughput sequencing. We found that the relative abundances of specific microorganisms decreased after different cultivation times, e.g., Sphingomonas, Pseudomonas, Rhodanobacter, and Caulobacter, which have been reported as plant-growth beneficial bacteria. The relative abundances of potential plant pathogenic fungi Myrothecium and Ascochyta increased with the successive cultivation times. The co-occurrence network analysis showed that the bacterial and fungal communities under maize were much more stable than those under rice, soybean, and wheat. The present study explored the characteristics of bacteria and fungi in crop seedling rhizosphere and indicated that the characteristics of indigenous soil flora might determine the plant growth status. Further study will focus on the use of the critical microorganisms to control the growth and yield of specific crops.
Abstract There are three major factors affecting the coastal environment in Taiwan: tidal current, long-shore current and land subsidence. in Taiwan, most industrial areas are located on the southwestern coast. Most of the pollutants from the factories are discharged directly to the ocean, harbours or rivers. the pollutants in harbours or rivers will eventually be delivered to coastal waters. There, the tidal current is the major force dispersing the pollutants nearshore. the polluted water extends to an area about 5 km on both sides from the discharge source, and within about 3–4 km off-shore. in this study, Hsien-Da Harbour in southwestern Taiwan is cited to explain the effects of tidal current on the coastal environment. the long-shore current induced by breaking waves is the major force shaping the coastal morphology. Along the western coast of Taiwan, the long-shore current mainly flows northward along the southern section, and southward along the northern section. in the last one hundred years, in general, the coast has been eroded along the northern and southern sections of western coast, and some reclaimed lands were formed in the middle section. Recently, erosion in some coastal zones is quite serious because of excess groundwater extraction, especially along the southwestern coast. Groundwater extraction might also induce land subsidence. There are some areas near the coast which suffer serious land subsidence problems. 相似文献