Shaping effects of rice,wheat, maize,and soybean seedlings on their rhizosphere microbial community

The rhizosphere microbiome plays critical roles in plant growth and is an important interface for resource exchange between plants and the soil environment. Crops at various growing stages, especially the seedling stage, have strong shaping effects on the rhizosphere microbial community, and such community reconstruction will positively feed back to the plant growth. In the present study, we analyzed the variations of bacterial and fungal communities in the rhizosphere of four crop species: rice, soybean, maize, and wheat during successive cultivations (three repeats for the seedling stages) using 16S rRNA gene and internal transcribed spacer (ITS) high-throughput sequencing. We found that the relative abundances of specific microorganisms decreased after different cultivation times, e.g., Sphingomonas, Pseudomonas, Rhodanobacter, and Caulobacter, which have been reported as plant-growth beneficial bacteria. The relative abundances of potential plant pathogenic fungi Myrothecium and Ascochyta increased with the successive cultivation times. The co-occurrence network analysis showed that the bacterial and fungal communities under maize were much more stable than those under rice, soybean, and wheat. The present study explored the characteristics of bacteria and fungi in crop seedling rhizosphere and indicated that the characteristics of indigenous soil flora might determine the plant growth status. Further study will focus on the use of the critical microorganisms to control the growth and yield of specific crops.

     

Metabolomic modulations in a freshwater microbial community exposed to the fungicide azoxystrobin

An effective broad-spectrum fungicide, azoxystrobin (AZ), has been widely detected in aquatic ecosystems, potentially affecting the growth of aquatic microorganisms. In the present study, the eukaryotic alga Monoraphidium sp. and the cyanobacterium Pseudanabaena sp. were exposed to AZ for 7 days. Our results showed that 0.2–0.5 mg/L concentrations of AZ slightly inhibited the growth of Monoraphidium sp. but stimulated Pseudanabaena sp. growth. Meanwhile, AZ treatment effectively increased the secretion of total organic carbon (TOC) in the culture media of the two species, and this phenomenon was also found in a freshwater microcosm experiment (containing the natural microbial community). We attempted to assess the effect of AZ on the function of aquatic microbial communities through metabolomic analysis and further explore the potential risks of this compound. The metabonomic profiles of the microcosm indicated that the most varied metabolites after AZ treatment were related to the citrate cycle (TCA), fatty acid biosynthesis and purine metabolism. We thereby inferred that the microbial community increased extracellular secretions by adjusting metabolic pathways, which might be a stress response to reduce AZ toxicity. Our results provide an important theoretical basis for further study of fungicide stress responses in aquatic microcosm microbial communities, as well as a good start for further explorations of AZ detoxification mechanisms, which will be valuable for the evaluation of AZ environmental risk.     

Evaluation of phoxim toxicity on aquatic and zebrafish intestinal microbiota by metagenomics and 16S rRNA gene sequencing analysis

Environmental Science and Pollution Research - Phoxim is one of the main organophosphorus pesticides used in agricultural production. However, little information is known about how it affects the...     

Effects of UV-modified biochar derived from phytoremediation residue on Cd bioavailability and uptake in Coriandrum sativum L. in a Cd-contaminated soil

Environmental Science and Pollution Research - Biochar has been applied widely as an amendment in the remediation of contaminated soil to immobilize the heavy metals. However, the role of...     

Alteration of dominant cyanobacteria in different bloom periods caused by abiotic factors and species interactions

Freshwater cyanobacterial blooms have drawn public attention because they threaten the safety of water resources and human health worldwide. Heavy cyanobacterial blooms outbreak in Lake Taihu in summer annually and vanish in other months. To find out the factors impacting the cyanobacterial blooms, the present study measured the physicochemical parameters of water and investigated the composition of microbial community using the 16S rRNA gene and internal transcribed spacer amplicon sequencing in the months with or without bloom. The most interesting finding is that two major cyanobacteria, Planktothrix and Microcystis, dramatically alternated during a cyanobacterial bloom in 2016, which is less mentioned in previous studies. When the temperature of the water began increasing in July, Planktothrix appeared first and showed as a superior competitor for M. aeruginosa in NO₃^?-rich conditions. Microcystis became the dominant genus when the water temperature increased further in August. Laboratory experiments confirmed the influence of temperature and the total dissolved nitrogen (TDN) form on the growth of Planktothrix and Microcystis in a co-culture system. Besides, species interactions between cyanobacteria and non-cyanobacterial microorganisms, especially the prokaryotes, also played a key role in the alteration of Planktothrix and Microcystis. The present study exhibited the alteration of two dominant cyanobacteria in the different bloom periods caused by the temperature, TDN forms as well as the species interactions. These results helped the better understanding of cyanobacterial blooms and the factors which contribute to them.     

基于BP神经网络不同输入变量的爆破振速预测

以某隧道爆破开挖为实例,利用BP神经网络解决复杂非线性函数逼近问题的能力,以最大段药量、爆心距、爆破分段数、泊松比、岩石基本质量指标作为影响爆破振动速度的主要因素,选取不同维数的输入变量建立BP神经网络模型来预测爆破振动速度。对比分析各组预测值与实测值之间的相对误差,选取合理维数的输入变量建立了爆破振动危害预测的BP神经网络模型。     

内蒙古典型草原土壤不同剖面深度CO_2通量格局及其驱动因子

土壤CO2的释放能够显著增加大气牛CO2的浓度,增强温室效应,从而对全球气候和环境变化产生重要影响.但是,不同的土壤层对CO2通量的贡献量有很大的差异.文章通过挖坑法结合红外气体分析法研究了内蒙古草原典犁针茅(Stipa krylovii)群落和羊草(Leymus chinensis)群落不同剖面深度土壤CO2通量格局以及影响CO2通量的驱动因素.结果表明,表层土壤移走后,土壤CO2通量的变化可分为瞬时、短期、长期三种格局.新剖面上最初的0～21 min内释放的CO2通量最均大于初始土壤表层CO2通量,而且两者比值随土壤深度增加而增大,也随土壤CO2生产能力增强而增大.2～4 d后,新剖面CO2通量持续下降至低于初始土壤表层CO2通量的水平.形成短期稳定状态.更长时间后,新剖面则逐渐表现出与初始土壤剖面表层相近的CO2通量特征.我们认为,(1)在新剖面形成时的CO2通量瞬时和短期格局主要受土壤中存留的原始CO2的浓度及其扩散过程控制,(2)长期格局则由资源水平和环境条件共同决定的土壤CO2生产能力主导.文章进一步揭示了建立包含垂直分层的SOC分解和CO2扩散过程的生态系统模型的必要性.     

一次中尺度对流系统的发生发展特征分析

通过分析2008年6月23日形成于冷涡成熟阶段的中尺度对流系统(MCS)的发生发展特征,得到:(1)MCS发生发展过程中,高空强垂直风切变维持,低层垂直风切变迅速增大。(2)湿Q矢量的低层辐合、高层辐散,加强了上升运动和次级环流,前者的增大对MCS的发展起了更大的作用。(3)雷达图上弱回波区、回波悬垂结构、三体散射现象、大的垂直累积液态水及低层速度图上的气旋式辐合的出现是冰雹发生前的有利信号。(4)湿斜压性及风垂直切变增大可能会促发MCS发生。地面风场辐合和水汽辐合加强了MCS的发展。对流层中高层干冷空气的侵入,使不稳定能量释放,导致了强对流天气的发生。     

山西省城市居民环境意识调查分析

山西省城市环境质量状况虽有了较大的改善,但任务仍十分艰巨,保护当地的环境资源改善人们的环境生存状况是当前的重要任务之一。本研究通过问卷调查的形式,从环境认知、环境意向、环境行为等方面,调查了解山西省城市居民的环境意识现状及存在的主要问题,并在此基础上进行了深入的分析,提出了提高居民环境意识,促进环境质量改善的建议。