Characterization of On-Road Vehicle NO Emissions by a TILDAS Remote Sensor

ABSTRACT

A tunable infrared laser differential absorption spectrometer (TILDAS) was used to remotely sense the nitric oxide (NO) emissions from 1,473 on-road vehicles. The real-world measurement precision of this instrument in the limit of low NO concentration is 5 ppm of the vehicle exhaust, which corresponds to a 3o detection limit of 15 ppm. Our analysis of the distribution of negative concentration measurements produced during this experiment supports this claim, showing that the instrumental noise for this set of measurements was at most 8 ppm in the limit of low NO concentration. The high sensitivity of this instrument allowed us to measure the NO emissions of even the cleanest vehicles. The measured vehicle fleet NO emissions closely fit a gamma distribution with 10% of the fleet contributing about 50% of the total fleet emissions. Newer vehicles had lower NO emissions than older ones, but high NO emitters were found in every vehicle age cohort. On a vehicle-by-vehicle basis, NO emissions correlated very weakly with vehicle velocity, acceleration, power per unit mass, carbon monoxide (CO) emissions, and hydrocarbon (HC) emissions. High NO emitting vehicles could not be identified by remote sensing of CO or HC emissions and vice versa. When we compared the NO emissions for 117 vehicles measured more than one time, about half of the high NO emitters were found to be very consistent, while the other half varied significantly.     

The effect of paraoxon on spermatogenesis in <Emphasis Type="Italic">Dugesia gonocephala</Emphasis> from the Chilean Altiplano: proliferation and apoptosis

Introduction and aims  

The Chilean Altiplano ecosystem is conserved free from contaminants and pollutants because of the absence of major local human activities such as agriculture or other industries. We studied the effects of paraoxon on proliferation and apoptosis of testicular cells during active spermatogenesis in Dugesia gonocephala collected from a pristine river (Guacollo) in the Altiplano region nearby Visviri town, Chile.     

Noninvasive prenatal diagnosis in a family at risk for Fraser syndrome

The front cover image is based on the Research Letter Noninvasive prenatal diagnosis in a family at risk for Fraser syndrome by Aldesia Provenzano et al., https://doi.org/10.1002/pd.5700 .

     

Constructing sustainable palm oil: how actors define sustainability

Biofuels as a renewable source of energy have gained considerable importance in recent years. The use of biofuels is expected to rise since national governments of developed nations like the US and European countries see it as one of the ways to fulfill climate targets and increase the security in their energy supply. Production of biofuels is also expected to rise as developing nations see in biofuels the opportunity for connecting to international markets through supplying a new demand in the energy market.Several studies report on the environmental, social and economic gains and detriments that can arise from increased biofuel production and consumption. However, research that provides insight into the way in which such issues are defined by actors within the product chain is scarce. In this article we analyse how the strategies and value definitions of actors involved in the production and consumption of biofuels lead to specific definitions of sustainability. The empirical material concerns the chain of palm oil production in Colombia and electricity generation in the Netherlands. It is analysed using the method of action-in-context, which allows us to uncover the level and source of diversity of sustainability definitions in the product chain.While the current growth in production of palm oil is definitely buyer driven, the analysis of various activities in the chain shows that several aspects of sustainability are defined in more complex actor fields throughout the product chain.     

Recovery of Francisella tularensis from soil samples by filtration and detection by real-time PCR and cELISA

The aim of this study was to develop a specific and highly sensitive method able to detect very low concentrations of Francisella tularensis in soil samples by real-time PCR (qPCR) with SYBR Green I. tul4 gene, which encodes the 17-kDa protein (TUL4) in F. tularensis strains, was amplified using a LightCycler (LC) device. We achieved a detection limit of 0.69 fg of genomic DNA from F. tularensis subp. holarctica live vaccine strain (LVS), corresponding to a value less than 3.4 genome equivalents per reaction. The qPCR was shown to be specific, highly sensitive and reproducible. In addition, we evaluated 2 new methods for recovering bacteria from soil based on 1-step filtration using glass fiber filters and PVDF filters. These filtration methods enabled us to recover F. tularensis efficiently from soil samples. As few as 50 CFU per 0.5 g of soil were detected by qPCR. Capture enzyme-linked immunosorbent assay (cELISA) allowed us to detect and quantify the amount of bacteria recovered from soil by an immunological method. Although qPCR was more sensitive than cELISA, we did not observe substantial differences in the amount of bacteria quantified by both methods.     

Texture Analysis for Mapping Tamarix parviflora Using Aerial Photographs along the Cache Creek, California

Natural color photographs were used to detect the coverage of saltcedar, Tamarix parviflora, along a 40 km portion of Cache Creek near Woodland, California. Historical aerial photographs from 2001 were retrospectively evaluated and compared with actual ground-based information to assess accuracy of the assessment process. The color aerial photos were sequentially digitized, georeferenced, classified using color and texture methods, and mosaiced into maps for field use. Eight types of ground cover (Tamarix, agricultural crops, roads, rocks, water bodies, evergreen trees, non-evergreen trees and shrubs (excluding Tamarix)) were selected from the digitized photos for separability analysis and supervised classification. Due to color similarities among the eight cover types, the average separability, based originally only on color, was very low. The separability was improved significantly through the inclusion of texture analysis. Six types of texture measures with various window sizes were evaluated. The best texture was used as an additional feature along with the color, for identifying Tamarix. A total of 29 color photographs were processed to detect Tamarix infestations using a combination of the original digital images and optimal texture features. It was found that the saltcedar covered a total of 3.96 km² (396 hectares) within the study area. For the accuracy assessment, 95 classified samples from the resulting map were checked in the field with a global position system (GPS) unit to verify Tamarix presence. The producer's accuracy was 77.89%. In addition, 157 independently located ground sites containing saltcedar were compared with the classified maps, producing a user's accuracy of 71.33%.     

Sustainable sanitary landfills for neglected small cities in developing countries: The semi-mechanized trench method from Villanueva,Honduras

Open dumping is the most common practice for the disposal of urban solid wastes in the least developed regions of Africa, Asia and Latin America. Sanitary landfill design and operation has traditionally focused on large cities, but cities with fewer than 50,000 in population can comprise from 6% to 45% of a given country’s total population. These thousands of small cities cannot afford to operate a sanitary landfill in the way it is proposed for large cities, where heavy equipment is used to spread and compact the waste in daily cells, and then to excavate, transport and apply daily cover, and leachate is managed with collection and treatment systems. This paper presents an alternative approach for small cities, known as the semi-mechanized trench method, which was developed in Villanueva, Honduras. In the semi-mechanized trench method a hydraulic excavator is used for 1–3 days to dig a trench that will last at least a month before it is filled with waste. Trucks can easily unload their wastes into the trench, and the wastes compact naturally due to semi-aerobic biodegradation, after which the trenches are refilled and covered. The exposed surface area is minimal since only the top surface of the wastes is exposed, the remainder being covered by the sides and bottom of the trench. The surplus material from trench excavation can be valorized for use as engineering fill onsite or off. The landfill in Villanueva has operated for 15 years, using a total land area of approximately 11 ha for a population that grew from 23,000 to 48,000, with a land requirement of 0.2 m²/person year, a cover to waste ratio of 0.2, and an estimated soil surplus of 298,000 m³ that is valorized and used onsite. The landfill has been operated solely by the municipality with an operational cost in 2010 estimated at US$4.60 per ton. A modified water balance analysis at Villanueva shows negligible leachate generation from covered trenches and 700 m³/yr (60 m³/ha yr) from the two open trenches required for daily operation. If the site were an open dump, however, leachate generation is estimated to be 3900 m³/ha yr and contaminated runoff 5000 m³/ha yr. A simple model used to estimate dilution of generated leachate based on groundwater flow data and aquifer stratigraphy suggests that the leachate will be diluted by a factor of 0.01 in the aquifer. Leachate contaminants will not accumulate because the aquifer discharges to the Ulua River 2 km south of the landfill. While not suitable for all sites, the Villanueva method nevertheless serves as an excellent example of how a small city landfill with natural compaction of waste and attenuation of leachate can be sustainably operated.     

PCBs,PCDDs and PCDFs in soil samples from uncontrolled burning of waste electrical material for metal reclamation

PCDDs, PCDFs and PCBs have been analysed in soil samples where waste electrical equipment has been burned directly on the ground in three locations of Burgos (Spain). High levels of PCDDs, PCDFs and PCBs were detected in the centre of combustion sites. The results show PCB levels between 0.824 and 1.240 μg/g, and PCDDs and PCDFs levels between 8.73–36.37 ng/g and 12.79–50.95 ng/g. The I‐TEF values between 2.96–9.15 for dioxins and furans indicate that these soils are heavily polluted and may represent a risk for human and animal health.