水产品中甲氰菊酯残留的ELISA快速检测

为快速检测水产品中甲氰菊酯的残留,应用水溶性碳化二亚胺(EDC)法将甲氰菊酸与牛血清白蛋白(BSA)和卵清蛋白(OVA)偶联,合成了甲氰菊酯的人工免疫抗原和包被抗原.免疫抗原经紫外扫描和聚丙烯酰胺电泳验证构建成功,用此抗原免疫试验兔子,获得了甲氰菊酯的多克隆抗体.经检测,抗体对甲氰菊酯具有较好的特异性和灵敏性.在此基础上建立了水产品中甲氰菊酯残留的间接ELISA检测法.理想的甲氰菊酯-OVA包被抗原质量浓度为1.6 mg/L,酶标二抗(羊抗兔IgG-HRP)的稀释度为1∶1000,多抗的稀释度为1∶3 200,最适检测范围为20～200 μg/kg,在实际样品检测时的最低检出限为20 μg/kg,接近或达到气相色谱法的测定水平,但检测速度大大提高,可在3 h内完成近百个样品的分析,适合甲氰菊酯的快速检测.     

Development of analytical methods for polycyclic aromatic hydrocarbons （PAHs） in airborne particulates： A review

In the present work, the different sample collection, pretreatment and analytical methods for polycyclic aromatic hydrocarbons （PAHs） in airborne particulates is systematacially reviewed, and the applications of these pretreatment and analytical methods for PAHs are compared in detail. Some comments on the future expectation are also presented.     

GC/MS法测定生物样品中多溴联苯醚类化合物

本研究建立了多溴联苯醚（PBDEs）生物样品分析方法,获得了优化的前处理条件、气相色谱和质谱检测条件.对定性定量方法及实验条件必须满足的要求进行了评价.通过质量控制（quality control, QC）对整个方法进行了验证.结果证明,本研究所建立的分析方法满足生物样品PBDEs测定的要求,回收率在50.5%～112.3%之间,回收率相对标准偏差在5.3%～9.9%之间,方法检测限在7.1～161.8 pg/g之间.在建立的方法基础上,测定了部分养殖和野生鱼类样品,测得的野生鳜鱼和鲫鱼以及2个养殖鲈鱼样品的结果分别为1.53、1.11、5.31和6.15 ng/g(干重),其中主要的同族体为BDE47.     

海水重金属监测分析方法研究

总结国内外的海水水质重金属的监测分析方法,调查国内海水重金属分析方法,比较各种分析方法的优缺点.得到以下结论：海水中汞、砷、硒的主要分析方法为原子荧光法,此方法具有操作简单、灵敏度高等优点;铜、铅、锌、镉、镍的主要分析方法为原子吸收法,此方法比极谱法具有更好的灵敏度和重现性;总铬和六价铬的分析方法主要为二苯碳酰二肼分光光度法,方法操作简单、易于推广.     

基于层次分析法的秦皇岛市垃圾填埋场选址评价

城市垃圾填埋场场址的选择，受到当地经济、交通、气候条件，自然地理条件，环境保护条件，水文地质工程地质条件等诸多因素影响，是一项十分复杂的工程。本文总结了秦皇岛市现有垃圾填埋场的基本情况、存在问题及其产生原因。鉴于层次分析法在多目标决策分析方面的成效，将其运用到秦皇岛市垃圾填埋场选址评价中，对研究区进行适宜性分区，依次划分为最佳场地区、适宜场地区、较适宜场地区、不适宜场地区。     

AHP法在废水治理工程评价中的应用

应用层次分析法（ＡＨＰ）的原理，建立了废水处理工程的评价模型，并结合废水处理的实例进行了研究，结果证明了ＡＨＰ法是评价废水处理工程的有效方法。     

DISTRIBUTED DISCHARGE OF COOLING WATER ALONG DIRECTION OF STREAM FLOW1

ABSTRACT. A multiple-outfall system for cooling water disposal which is distributed along the direction of stream flow is analyzed. The results provide a simple method of preliminary design of a cooling water discharge outfall system and for a quick extimate of water quality of a polluted stream. It is shown that a properly designed distributed discharge system is capable of preventing serious pollution due to localized discharge of wastes and waste heat; however, the final design of the discharge system should take into account alternative schemes of waste heat disposal and their economic consequences.     

EVALUATING INLAND WATERWAY NAVIGATION IMPROVEMENT PROJECTS: THE CASE OF THE BONNEVILLE LOCK

ABSTRACT: The period of time from identification of a possibly desirable inland waterway transportation project to its actual implementation has been observed to be inordinately long. It is Hypothesized that at Least one cause of delays in project approval and implementation is a analytical credibility associated with project feasibility analysis conducted by the U.S. Army Corps of Engineers. This paper examines this hypothesis in terms of the proposal to construct a new, expanded lock at the Bonneville Dam on the Columbia River. The principle finding is that the analysis conducted by the Corps is conceptually flawed, methodologically questionable, internally inconsistant, and inappropriately narrow in scope.     

Laboratory longitudinal diffusion tests: 2. Parameter estimation by inverse analysis

This study focuses on the verification of test interpretations for different state analyses of diffusion experiments. Part 1 of this study identified that steady, quasi-steady and equilibrium state analyses for the through- and in-diffusion tests with solution reservoirs are generally feasible where the tracer is not highly sorptive. In Part 2 we investigate parameter identifiability in transient-state analysis of reservoir concentration variation using a numerical approach. For increased generality, the analytical models, objective functions and Jacobian matrix necessary for inverse analysis of transient-state data are reformulated using unified dimensionless parameters. In these dimensionless forms, the number of unknown parameters is reduced and a single dimensionless parameter represents the sorption property. The dimensionless objective functions are evaluated for individual test methods and parameter identifiability is discussed in relation to the sorption property. The effects of multiple minima and measurement error on parameter identifiability are also investigated. The main findings are that inverse problems for inlet and outlet reservoir concentration analyses are generally unstable and well-posed, respectively. Where the tracer is sorptive, the inverse problem for the inlet reservoir concentration analysis may have multiple minima. When insufficient measurement data is collected, multiple solutions may result and this should be taken into consideration when inversely analyzing data including that of inlet reservoir concentration. Verification of test interpretation by cross-checking different state analyses is feasible where the tracer is not highly sorptive. In an actual experiment, test interpretation validity is demonstrated through consistency between theory and practice for different state analyses.     

航空维修人为差错影响因素分析中的模糊层次分析法

航空维修中的人为差错影响因素分析对于预防事故发生至关重要,如何定量分析及辨识出主要影响因素是亟待解决的问题。为此,结合航空维修实际,提出运用群组模糊层次分析法对人为差错影响因素进行量化排序和分类,归纳细化了影响因素层次体系,并给出了分析计算流程。以一起由维修人为差错所引发的航空事故为例进行了实证分析,结果表明:该方法能够辨识出航空维修中人为差错产生的主要影响因素,进而对制订最优事故预防方案,控制和减少该类事故的发生有积极意义。同时该方法对其他行业中的人为差错主要影响因素辨识有着一定的参考价值。