Isolation of marine benzo[a]pyrene-degrading Ochrobactrum sp. BAP5 and proteins characterization

A bacterial strain BAP5 with a relatively high degradation ability of benzo[a]pyrene (BaP) was isolated from marine sediments of Xiamen Western Sea, China and identified as Ochrobactrum sp. according to 16S rRNA gene sequence as well as Biolog microbial identification system. Strain BAP5 could grow in mineral salt medium with 50 mg/L of BaP and degrade about 20% BaP after 30 d of incubation. Ochrobactrum sp. BAP5 was able to utilize other polycyclic aromatic hydrocarbons (PAHs) (such as phenanthrene, pyrene and fluoranthene) as the sole carbon source and energy source, suggesting its potential application in PAHs bioremediation. The profile of total soluble protein from Ochrobactrum sp. BAP5 was also investigated. Some over- and special-expressed proteins of strain BAP5 when incubated with the presence of BaP were detected by two-dimensional polyacrylamide gel electrophoresis, and found to be related with PAHs metabolism, DNA translation, and energy production based on peptide fingerprint analysis through matrix-assisted laser desorption/ionization-time of flight mass spectrometry.     

High-resolution modeling and evaluation of ozone air quality of Osaka using MM5-CMAQ system

High-resolution modeling approach is increasingly being considered as a necessary step for improving the monitoring and predictions of regional air quality. This is especially true for highly urbanized region with complex terrain and land-use. This study uses Community Multiscale Air Quality (CMAQ) model coupled with MM5 mesoscale model for a comprehensive analysis to assess the suitability of such high-resolution modeling system in predicting ozone air quality in the complex terrains of Osaka, Japan. The 1-km and 3-km grid domains were nested inside a 9-km domain and the domain with 1-km grid covered the Osaka region. High-resolution Grid Point Value-Mesoscale Model (GPV-MSM) data were used after suitable validation. The simulated ozone concentrations were validated and evaluated using statistical metrics using performance criteria set for ozone. Daily maxima of ozone were found better simulated by the 1-km grid domain than the coarser 9-km and 3-km domains, with the maximum improvement in the mean absolute gross error about 3 ppbv. In addition, 1-km grid results fared better than other grids at most of the observation stations that showed noticeable di erences in gross error as well as correlation. These results amply justify the use of the integrated high-resolution MM5-CMAQ modeling system in the highly urbanized region, such as the Osaka region, which has complex terrain and land-use.     

Heavy metal removal from aqueous solution using carbonaceous K2S-impregnated adsorbent

A novel carbonaceous adsorbent for heavy metal removal was prepared from raw coal by one-step simple sulfur impregnation using K2S. Raw coal was mixed with K2S powder and then heated at 800°C for 30 min in nitrogen to produce K2S char. The sulfur content and form in K2S char were determined, and the ability of K2S char to adsorb Zn2+, Cd2+ and Pb2+ was examined. The K2S impregnation was e ective at impregnating sulfur into coal, especially in the form of elemental, thiophenic and sulfatic sulfur. The sulfur content of K2S char was higher than those of raw coal and pyrolysis char. The Zn2+ removal in 2.4 mmol/L of Zn2+ solution by K2S char was higher than raw coal with the removal rate of 100%. K2S char adsorbed Pb2+ and Cd2+ in 24 mmol/L of Pb2+ and Cd2+ solution with the removal rate of 97% and 35%, respectively. The elution extents of adsorbed Pb2+ and Cd2+ were zero in distilled water and 27% in 0.1 mol/L HCl solution. These results indicated that an e ective adsorbent for heavy metal ions was prepared from coal using K2S sulfur impregnation, and that the adsorbed metals were strongly retained in K2S char.     

钙镁磷肥生产富氧空气助燃节能可行性分析

介绍了钙镁磷肥传统生产方法,分析了钙镁磷肥高炉富氧空气助燃的可行性,认为该方法可行。     

改性锯末吸附含Cr（Ⅵ）废水的实验研究

初步探讨了以改性后的锯末作为吸附剂去除废水中的Cr（Ⅵ）,实验表明改性锯末对于不同浓度的含Cr（Ⅵ）废水都具有较高的去除率。同时通过实验研究了pH值、吸附时间、吸附剂量、初始浓度等因素对吸附去除率的影响。     

环境空气SO2自动监测仪的维护

分析环境空气二氧化硫（SO2）自动监测仪故障产生原因,提出判断和处理故障的维修方法。     

流化BAF处理生活污水的研究

曝气生物滤池是一种新型的水处理技术。选择海绵块作为曝气生物滤池的填料,研究了此种海棉块在曝气生物滤池内的挂膜效果．讨论了不同的水力停留时间对曝气生物滤池处理生活污水效果的影响。结果表明,水力停留时间对处理效能有较明显的影响．缩短水力停留时间使反应器对COD、氨氮和悬浮物的去除率下降。     

富营养化水体中微囊藻毒素（MCs）去除技术研究进展

当前,随着工业的发展水体富营养化程度日益严重。引起富营养化的主要藻类一蓝藻,能够释放对人体及鱼类具有多器官毒性、遗传性和致癌性的毒素——微囊藻毒素（MCs）。MCs治理技术种类繁多,传统的物理法如：混凝沉淀、膜过滤、活性炭吸附、气浮以及直接过滤只能对简单的处理细胞内的MCs;化学氧化法、光催化氧化法以及高级氧化法虽然能有效处理水体中的MCs．但有成本高、易二次污染和操作复杂等缺点,难以满足日益严格的环保要求;生物酶法除了具有生物降解法所具有的廉价、无二次污染、降解彻底和易操作等特点外,还被刺用来监控水体中MCs,它将成为今后重点研究的技术领域．     

虚拟专用网络在江苏环境自动监测领域的应用

通过研究虚拟专用网络（VPN）在环境监测领域中的使用现状,结合江苏省自动站直联中国环境监测总站集成项目的设计方案与多个环境自动监控数据采集平台的运用,阐明了VPN网络在环境监测领域应用的优、缺点,同时为完善虚拟专业网络在环境监测领域的应用提出了对策和建议。     

CE-QUAL-W2模型应用探讨

通过二维纵向横向平均水动力学和水质模型(CE-QUAL-W2模型)对山仔水库进行水力学和水质的模拟,运用2006年4月至2006年5月的实际观测数据校正模型参数,并对山仔水库不同流量下的水质情况进行预测.模拟结果显示:温度分布在水库水体中存在空间和时间的异质性:水质重要组分如溶解氧,浮游生物量,总溶解固体,正磷酸盐,硝酸盐氮等的模拟值同观测值非常相近:调节水库的流量对改善水体水质情况有很大的作用.