Sex-biased lactational duration in a human population and its reproductive costs

Summary We tested the proposition that among humans (1) differences in lactational duration result in differences in costs of reproduction even under rich nutritional conditions; and (2) elimination of factors postulated to favor male-biased parental care will be reflected in elimination or reversal of sex-biased care. To do so, we examined the relationship between lactational duration and fertility among Hutterites, a communal-living human population in which the levels of nutritional resources and fertility are high, breast-feeding is the norm, contraceptive use is limited and the collective social and economic system results in low resource variance among individuals. We demonstrate that even under good nutritional conditions, duration of nursing was a significant predictor of the length of time to next pregnancy and that nursing continued to suppress fertility after the resumption of menses. Moreover, we find that daughters were nursed longer than sons, leading to a longer interval to next pregnancy. We examine this uncommon, but not unique, finding of female-biased human parental care in the light of Hutterite social structure, and we explore the consistency of this finding with the most applicable models of parental investment. Correspondence to: S.W. Margulis     

Monitoring of Bt-Maize pollen exposure in the vicinity of the nature reserve Ruhlsdorfer Bruch in northeast Germany 2007 to 2008

Background, aim, and scope Basically, technological innovations are associated with benefits and risks. This is also true for the introduction of genetically modified organisms (GMO) into agriculture. In Germany, precautionary regulations currently demand isolation distances (i.?e. buffer zones) for the cultivation of genetically modified maize (Bt-maize) in the vicinity of conventional (150?m) and organic maize fields (300?m). The Bt-toxin may harm non-target organisms (NTO) such as Lepidoptera. Despite this, corresponding regulations for the protection of nature reserves are lacking to date. Conventional and Bt-maize have been grown in the vicinity of the Flora-Fauna-Habitat (FFH) Ruhlsdorfer Bruch in Brandenburg, Germany. The aim of this study was to investigate whether exposure of maize pollen from surrounding Bt-maize fields to NTOs in the nature protection area could be excluded or not. Two types of exposure were investigated: Firstly, whether maize pollen was dispersed by wind into the nature reserve area exposing resident NTOs. Secondly, foraging NTOs from the nature reserve are exposed by roaming the surrounding fields and collecting maize pollen. In order to fulfil the precautionary principle defined by law, the study should help to determine appropriate isolation distances for the cultivation of Bt-maize with regard to sustainable protection of NTOs in the FFH Ruhlsdorfer Bruch. In 2007, the local authorities issued an isolation distance between Bt-maize fields and nature reserves of 100?m and in 2008, this became 250?m in the northern and 500?m in the westerly direction, respectively. Materials and methods Standardised methods for biological and technical pollen sampling issued by the Association of German Engineers (VDI 4330 Part 3, 2007 and VDI 4330 Part 4, 2006) were applied providing a quality controlled and methodologically harmonised database which does not only serve the needs to be fulfilled by the present case-specific monitoring study but can also be used as a reference database for further investigations. Maize pollen exposure was measured within the FFH Ruhlsdorfer Bruch and its immediate vicinity in July and August 2007 and 2008. In 2007, the sampling was performed at three sites using 12 technical samplers (Sigma-2/PMF) placed at five measuring points at distances from 5?m to 120?m from the maize field edges. Additionally, for biological pollen sampling six bee colonies were situated at these three sites (two colonies at each site). The technical sampler Sigma-2/PMF enables point sampling which is primarily influenced by wind and topography providing information on effective maize pollen input (flow and deposition) at the measuring sites. Honey-bees roaming in the surrounding area with typical foraging distances of several kilometres may act as planar collectors. They may serve as indicators for the exposure to pollen-collecting NTOs. Furthermore, biological sampling is more selective due to the organism’s preferences, whereas the technical sampling is neutral. Hence, both the technical and the biological sampling complement each other in their scope of application. The pollen samples of both matrices were analysed microscopically and the maize pollen loads were quantified. Pollen-DNA was analysed by means of the quantitative PCR-method (qPCR) identifying conventional and Bt-maize pollen by two independent laboratories. In 2008, the monitoring was repeated with additional sites. Eighteen technical samplers were exposed at five sites with eight measuring points at distances from 5?m to 250?m from the maize field edges. Two honey-bee colonies for biological sampling were placed at one site for control purposes. PCR-analyses were performed to measure the amounts of Bt-maize pollen in the samples. Results The results of pollen monitoring at the Ruhlsdorfer Bruch revealed maize pollen exposure for all monitoring sites in both surveys. In 2007, up to 1.75 million maize pollen/m² were deposited at sites closest to the maize field. At 120?m from field edge in the middle of the FFH-reserve, 99,000 maize pollen/m² were detected. In 2008, similar results were found, at distances up to 250?m from the field edges deposition of 164,000 maize pollen/m² was detected. Data on maize pollen deposition show a clear distance relationship and are in accordance with results of further comprehensive surveys based on the same methodology (Hofmann 2007). The results of the microscopic analysis of the pollen pellets demonstrated that bees collected maize pollen at all sites, in 2007 and 2008. Although maize pollen is not the main food source (2007:0.1–0.3?%; 2008:2–3?%) the collection efficiency of the bee colonies resulted in high amounts of sampled maize pollen with 4 to 11 million per site in 2007 and up to 467 million in 2008. Molecular-biological analysis of maize pollen DNA by qPCR demonstrated that transgenic Bt-MON810 DNA was present in all technical and biological samples, corroborated by two independent laboratories. In 2007, the GMO-content in the samples ranged up to 44?% in the bioaerosols and 49?% in the pollen pellets. In 2008, GMO-proportions of up to 18?% were detected. Discussion The results of this study provide evidence that NTOs in the Ruhlsdorfer Bruch were exposed to Bt-maize pollen under the cultivation conditions in 2007 with a buffer zone of 100?m. The GMO-content reached up to 48?%. The results of the monitoring in 2008 confirmed these findings. Even though the exposure could be reduced by increasing the isolation distances to 250?m and 500?m respectively, the results still show percentages of up to 18?% Bt-MON810 in the pollen samples. The results on maize pollen deposition at the Ruhlsdorfer Bruch in 2007 and 2008 correspond to the results of an investigation which was conducted over several years applying the same standardised method, but covering a wider range of distances. The correlation between maize pollen deposition (n/m²) and distance to the source field (m) fitted best to a power function of the type y = 1.2086 · 10⁶ · x ^–0.548. Despite the same trend, the pollen deposition in the Ruhlsdorfer Bruch revealed above-average findings. Also the analysis of the pollen pellets collected by the bees showed an exposure in 2007 with values for the GMO-content of up to 49?%. For both methods, the exposure decreased in 2008 due to the greater buffer zones up to 500?m. Whereas the GMO-content for the biological sampling were reduced to values below 10?%, the values for the technical sampling were still higher indicating that greater buffer zones would be necessary for safety reasons under the precautionary principle. Conclusions The results of this investigation proved that maize pollen were dispersed by wind to distances farther than 250?m from field edge leading to maize pollen exposure in the centre of the nature reserve. The results also demonstrated that foraging NTOs living in the nature reserve were exposed to maize pollen from surrounding fields. Considering the cultivation of Bt-maize MON810, the assumption of the Environmental Risk Assessment (ERA) that there will be no relevant exposure beyond the Bt-maize fields, cannot be confirmed. Considering the results of this and related studies and with respect to the precautionary principle, one can state that buffer zones between Bt-maize fields and protected areas are an effective measure to minimise the exposure of Bt-maize pollen to NTOs and, thus, to prevent from adverse effects. Recommendations and perspectives Because of still insufficient ecotoxicological data for the risk assessment of Bt-maize MON810 considering butterflies and other protected NTOs, protection standards assuring the precautionary principle have to be implemented to avoid Bt-maize pollen exposure to NTOs. This applies for the case Ruhlsdorfer Bruch and for nature reserve areas in general. In order to exclude risks to protected NTOs occurring in nature reserves, sufficient buffer zones for Bt-maize cultivation should be considered. The statistical analysis revealed that distances of more than 500?m are necessary to decisively reduce exposure to foraging insects. In fact, distances of more than 1,000?m are necessary to prevent maize pollen deposition from values above 100,000 pollen/m² with a certainty of 90?%. An adequate risk assessment can only be attained if based on field measurements accounting for the high variation of pollen deposition due to local environmental site conditions and field management. The monitoring should be based on standardised methods. It should include locations with the highest expected deposition rates, the boundaries of the protected areas and sites of interest within those boundaries, e.?g., specific habitats of sensitive species.     

Die Entstehung der chemischen Elemente

For 25 years the conception has been well established that the overwhelming majority of chemical elements and their isotopes have been synthesized in stars during the course of stellar evolution, in particular during the advanced stages and in supernova explosions. Until now this general idea has not led to major difficulties or inconsistencies. On the contrary, the theoretical models have been very successful in explaining the observed abundances in meteorites, planets (including the earth), stars (including the sun) and galactic cosmic rays. Our present understanding of nucleosynthesis will be reviewed with special attention given to nucleosynthetic processes in primordial stars and supernovae. We will concentrate primarily on predictions which can be made for relative abundances rather than for absolute abundances, because the latter problem requires evolutionary models of the galaxy which are beyond the scope of this article.     

GVO-Pollenmonitoring zum Bt-Maisanbau im Bereich des NSG/FFH-Schutzgebietes Ruhlsdorfer Bruch

Aim and Background Precautionary regulations for the production of genetically modified crops (Gentechnik-Pflanzenerzeugungsverordnung, GenTPflEV 2008) provide isolation distances for the cultivation of genetically modified maize – Bt-maize – in the vicinity of conventional maize cultivation (150?m) as well as of organic maize cultivation (300?m). Nature reserves are not included. The aim of this study was to investigate whether maize pollen of surrounding fields were dispersed in the Flora-Fauna-Habitat (FFH) Ruhlsdorfer Bruch. In the vicinity of the nature reserve Bt-maize species MON 810 as well as conventional maize was grown. The survey should provide appropriate isolation distances for the cultivation of Bt-maize with regard to sustainable protection of non-target-organisms (NTO) in the Ruhlsdorfer Bruch. Materials and Methods The collection of maize pollen in the Ruhlsdorfer Bruch was carried out at three sampling sites in the FFH or rather in the immediate vicinity in July and August 2007 by means of twelve technical and three biological pollen samplers. The technical samplers Sigma-2/PMF enable point sampling. They are primarily influenced by wind and topography and provide information about the effective entry, the maize pollen flow and the maize pollen deposition at the location of the sampler. Honey bees roam over longer distances and are hence planar collectors. Thus, they also provide information about the plants blooming in a distinct area. Furthermore, the biological preferences during the collection are captured, whereas a technical sampler does not perform a species dependent selection. Hence, both the technical and the biological samplers complement one another in their scope of application. The pollen samples were identified microscopically, enumerated quantitatively, and the pollen-DNA was analysed by means of the PCR-method. Results All monitoring sites at the Ruhlsdorfer Bruch revealed maize pollen entries. The pollen deposition reached values of 1.75 million maize pollen/m² in the close-up range. The monitoring sites located 120?m within the protected area still featured 99,000 maize pollen/m². As shown by the statistical analysis, at a distance of 1,000?m still 28,000 maize pollen/m² must be expected. The results of the microscopic pollen analysis of the pollen pellets proved that the bees collect maize pollen at all three sites. Although maize pollen is not the main food source the high collection efficiency of the bees resulted in large amounts of introduced pollen. The biomolecular proof of pollen-DNA in the field samples was corroborated by analogue results for both the technical and biological pollen sampling by two independent laboratories. The results of these quality controlled analyses gave unambiguous evidence that under the cultivation conditions in 2007 an entry of Bt-maize pollen into the FFH Ruhlsdorfer Bruch was existent. Discussion The maize pollen deposition at the Ruhlsdorfer Bruch corresponds with additional supra-regional investigations which were conducted over several years with the same method. However, the pollen entries into the Ruhlsdorfer Bruch are above-average. Possible reasons are the size of the maize fields (>?10?ha), the geographical collocation between maize fields and protected area (lee location, through-shaped, thermal currents) as well as weather conditions and maize bloom during the sampling period July and August 2007. A distance of 1,000?m or more is necessary to avoid maize pollen deposition of more than 100,000/m² with a probability of 90?%. Conclusions The results of this investigation prove an entry of Bt-maize pollen into the FFH under the cultivation and weather conditions in summer 2007. According to the results of this and related studies and considering precautionary principle and the effect terms defined in the Federal Immission Protection Law, it becomes clear that reasonable isolation distances between Bt-maize fields and protected areas have to be introduced in order to minimise the entry of GM-maize pollen and to prevent adverse effects on protected NTOs. Recommendations Due to the fact that no toxicological investigations are available for the butterflies occurring in the FFH, highest protection standards should be implemented to avoid Bt-maize pollen entries into the Ruhlsdorfer Bruch. That implies that highest possible isolation distances for Bt-maize cultivation have to be considered, but at least a distance of 1,000?m is recommended. Following this a maize pollen deposition of more than 100,000 maize pollen/m² should be pevented with a certainty of 90?% as well as an exceeding exposure of foraging insects. Outlook By means of the pollen monitoring it becomes possible to survey empirically defined limits of pollen exposure. This should be performed in the nature reserve area at sites of expected maximum exposure, e.?g. at the boundaries of the FFH towards the maize fields and at exposed sites.     

Genetic differentiation among matrilines in social groups of rhesus monkeys

Summary In rhesus monkey populations, animals related by descent to some female comprise a matriline or genealogy. Data on blood protein polymorphisms in the Cayo Santiago rhesus colony indicate that allele frequency variations among matrilines in social groups are large. These variations occur despite high levels of outbreeding. Computer simulation analyses indicate that pedigree or linear effect account for much of the observed genetic differentiation among genealogies. A sampling with correlation model in which genealogy sizes and average kinship levels are parameters predicts among matriline genetic differentiation. This study indicates that substantial genetic substructure is present within rhesus social groups. Our analyses also predict that large variances in allele frequencies should be common among social or trait groups based on kinship relationships.     

Conserving alpha and beta diversity in wood-production landscapes

International demand for wood and other forest products continues to grow rapidly, and uncertainties remain about how animal communities will respond to intensifying resource extraction associated with woody bioenergy production. We examined changes in alpha and beta diversity of bats, bees, birds, and reptiles across wood production landscapes in the southeastern United States, a biodiversity hotspot that is one of the principal sources of woody biomass globally. We sampled across a spatial gradient of paired forest land-uses (representing pre and postharvest) that allowed us to evaluate biological community changes resulting from several types of biomass harvest. Short-rotation practices and residue removal following clearcuts were associated with reduced alpha diversity (−14.1 and −13.9 species, respectively) and lower beta diversity (i.e., Jaccard dissimilarity) between land-use pairs (0.46 and 0.50, respectively), whereas midrotation thinning increased alpha (+3.5 species) and beta diversity (0.59). Over the course of a stand rotation in a single location, biomass harvesting generally led to less biodiversity. Cross-taxa responses to resource extraction were poorly predicted by alpha diversity: correlations in responses between taxonomic groups were highly variable (−0.2 to 0.4) with large uncertainties. In contrast, beta diversity patterns were highly consistent and predictable across taxa, where correlations in responses between taxonomic groups were all positive (0.05–0.4) with more narrow uncertainties. Beta diversity may, therefore, be a more reliable and information-rich indicator than alpha diversity in understanding animal community response to landscape change. Patterns in beta diversity were primarily driven by turnover instead of species loss or gain, indicating that wood extraction generates habitats that support different biological communities.