Application of an ELISA for PCB 118 to the screening of dioxin-like PCBs in retail fish

A commercially available enzyme-linked immunosorbent assay (ELISA) kit was evaluated for the determination of toxic equivalents (TEQs) of dioxin-like polychlorinated biphenyls (PCBs) in retail fish. The ELISA was highly specific for 2,3',4,4',5-pentachlorobiphenyl (PCB 118), which is generally the most abundant dioxin-like PCB isomer found in fish. The quantitative limit of the ELISA (using 3,3',4'-trichloro-4-methoxybiphenyl as a surrogate standard for PCB 118) was 10 ng ml(-1) (125 pg assay(-1)) in the standard curve, corresponding to 50 pg PCB 118 g(-1) in the tested sample. Good recoveries of PCB 118 (78.7-112.3%) were obtained for spiked purified fish extracts according to the ELISA. Good linearity was also obtained in dilution tests using purified fish extracts. No significant interference of the matrix was observed in the ELISA when this purification procedure was used. Recovery tests in which PCB 118 was added to fish samples also resulted in acceptable recoveries (60.2-82.3%) in the ELISA following purification. The ELISA results for fish samples correlated well with the TEQ concentrations of dioxin-like PCBs obtained by high-resolution gas chromatography/high-resolution mass spectrometry (r = 0.92, n = 26). These data indicate that the ELISA kit is suitable for screening retail fish for the TEQs of dioxin-like PCBs.     

Potential of microalgae Bio-Coke as a sustainable solid fuel alternative to coal coke

Energy and environmental issues have triggered the search for new sources of green energy alternatives in recent years. Biofuel production from renewable sources is widely considered one of the most sustainable alternatives for environmental and economic sustainability. Microalgae are currently being promoted as one of the most promising liquid biofuel feedstocks due to their rapid growth, high lipid production capacity, and carbon–neutral cycle. In this study, whole microalgae cells were utilized as raw material to produce solid biofuel, i.e., Bio-Coke, and this study aimed to investigate the possibility of microalgae Bio-Coke as an alternative to coal coke. The results show that Bio-Coke can be produced from microalgae in the temperature range of 80–100 °C. The apparent density is between 1.253 and 1.261 g/cm³, comparable to the apparent density of lignocellulosic Bio-Coke. Additionally, the calorific value is higher than the calorific value of lignocellulosic Bio-Coke and within the range of the calorific value of subbituminous coal. Therefore, microalgae Bio-Coke can be utilized to replace coal coke usage in the future.

     

Aerosol optical properties derived from imultaneoussunphotometer and aureolemeter measurements in Lhasa

In order to obtain some aerosol properties in Tibetan Plateau atmosphere, simultaneous measurements of aerosols by use of a sunphotometer and an aureolementer were performed in the summer of 1998 in Lhasa(29.65°N, 91.13°E). Optical depth, volume size distribution and phase-function of aerosols were obtained from the measurements. Religious activities can have an effect on optical depth in Lhasa. The daily average aerosol optical depth(λ相似文献   

Nutrient dynamics and the eutrophication of shallow lakes Kasumigaura (Japan), Donghu (PR China), and Okeechobee (USA)

We compared the nutrient dynamics of three lakes that have been heavily influenced by point and non-point source pollution and other human activities. The lakes, located in Japan (Lake Kasumigaura), People's Republic of China (Lake Donghu), and the USA (Lake Okeechobee), all are relatively large (> 30 km2), very shallow (< 4 m mean depth), and eutrophic. In all three lakes we found strong interactions among the sediments, water column, and human activities. Important processes affecting nutrient dynamics included nitrogen fixation, light limitation due to resuspended sediments, and intense grazing on algae by cultured fish. As a result of these complex interactions, simple empirical models developed to predict in-lake responses of total phosphorus and algal biomass to external nutrient loads must be used with caution. While published models may provide 'good' results, in terms of model output matching actual data, this may not be due to accurate representation of lake processes in the models. The variable nutrient dynamics that we observed among the three study lakes appears to be typical for shallow lake systems. This indicates that a greater reliance on lake-specific research may be required for effective management, and a lesser role of inter-lake generalization than is possible for deeper, dimictic lake systems. Furthermore, accurate predictions of management impacts in shallow eutrophic lakes may require the use of relatively complex deterministic modeling tools.     

Impact and effectiveness of Bio-Coke conversion from biomass waste as alternative source of coal coke in Southeast Asia

Journal of Material Cycles and Waste Management - Growing concern over fossil fuel depletion and the environmental impact of greenhouse gases have driven the demand for solid biofuel energy as an...     

Effects of simetryne on growth of various freshwater algal taxa

The sensitivity of 56 algal strains, representing 7 taxonomic groups to the triazine herbicide, simetryne, was examined using EC50 values for growth. There was a wide range of values from 6.5 to 1500 microg litre(-1). The Volvocales (Chlorophyceae, Chlorophyta) and Cyanophyceae (Cyanophyta) as a whole were the most sensitive, whereas the Desmidiales (Charophyceae, Chlorophyta) and Bacillariophyceae (Chromophyta) were the most tolerant, although sensitivity differed among strains of a single species. Sensitive and tolerant species were both isolated from samples collected at the same site. The results suggest that changes in species composition and relative abundance will occur when herbicides are applied in natural habitats.     

Hydrologic connection between ponds positively affects macrophyte alpha and gamma diversity but negatively affects beta diversity

Connections between habitat patches can positively influence the number of species in respective patches, providing a basis for preferentially conserving interconnected patches. However, from a regional perspective, it is not known whether conserving multiple sets of interconnected habitat patches would include more species (i.e., show higher gamma diversity) than conserving multiple, unconnected, solitary patches. We studied aquatic macrophytes in 15 sets of unidirectionally interconnected ponds and 19 unconnected ponds and also tested whether alpha and beta diversity, expressed as the number of species and dissimilarity in species composition, respectively, differed between connected and unconnected ponds. We found that gamma diversity was higher in connected ponds than in unconnected ponds, even after controlling for surface area. This resulted from a higher alpha diversity in connected ponds, despite lower beta diversity. These results suggest that connections between habitat patches positively influence diversity at both local and regional scales. When the total surface area available for conservation is limited, interconnected habitat patches should be preferentially conserved.