Root penetration through sealing layers at mine deposit sites.

To prevent acid mine drainage arising from oxygen and water penetration of sulphide-rich mine tailings, the tailings are covered with layers of dry sealing material. Plant roots have a great ability to penetrate dense materials, and if the roots are able to penetrate the sealing layer of a tailings deposit, its oxygen-shielding properties could be reduced. The objective of this study was to evaluate whether plant roots are able to penetrate sealing layers covering mine tailings deposits. Root penetration into layers of various sealing materials, such as clayey moraine (clay, 8-10%; silt, 22-37%; sand, 37-55%; gravel, 15-18%), moraine (unspecified), 6-mm bentonite (kaolin clay) fabric, lime and clay, Cefyll (mixture of pulverized coal fly ash, cement and water) and a mixture containing biosludge (30-35%) and bioashes (65-70%), was investigated. In the field, roots were studied by digging trenches alongside vegetation growing in 3- and 10-year-old mine sites. In the greenhouse root growth of Betula pendula, Pinus sylvestris, Poa pratensis and Salix viminalis were studied in compartments where the plants had been growing for 22 months. The results from the field experiment indicated that roots are able to penetrate both deep down in the cover layer (1.7 m) and also into the sealing layers of various materials, and even to penetrate hard Cefyll. The addition of nutrients in the top cover reduced deep root growth and thereby also penetration through the sealing layer. Low hydraulic conductivity of the sealing layer or a thick cover layer had less effect on root penetration. In the greenhouse experiment roots did not penetrate the thin bentonite fabric, due to low pH (2.1-2.7) that was created from the underlying weathered mine tailings. The clayey moraine was penetrated by all species used in the greenhouse experiment; Pinus sylvestris had the greatest ability to penetrate. To prevent root penetration of the other sealing layer, a suitable condition for the plants should be created in the upper part of the cover layer, namely a sufficient amount of plant nutrients. However, to define such a condition is difficult since different plant species have different requirements.     

Fetal cardiac anomalies and genetic syndromes

Cardiac anomalies may occur in isolation or can be part of a genetic syndrome. In this article, we describe some of the genetic syndromes commonly associated with cardiac anomalies where there are other sonographic features that may aid accurate prenatal diagnosis. Copyright © 2004 John Wiley & Sons, Ltd.     

Successful DNA-based prenatal exclusion of juvenile neuronal ceroid lipofuscinosis

A family with two siblings, 10 and 8 years old, both with clinical and ultrastructural evidence of juvenile neuronal ceroid lipofuscinosis is described. The family was found to be informative for the restriction fragment length polymorphisms (RFLPs) detected by the probes pCJ52–95Ml (locus D16S148) and pCJ52-94Tl (locus D16S159) flanking the juvenile neuronal ceroid lipofuscinosis locus, CLN3. The parents were both heterozygous using these probes, while their two children with juvenile neuronal ceroid lipofuscinosis were both homozygous. Chorionic villi analysis showed that the fetus was heterozygous and had inherited the one allele of the mother which was not found in the two siblings. This suggested that the fetus had derived one healthy allele from the mother, the risk for a double crossing-over being less than 1 per cent. Electron microscopy showed no fingerprint inclusions in chorionic villi. The child was investigated at 6 months of age and found to be healthy, as new fingerprint inclusions were found at electron microscopy and no vacuolated lymphocytes were found in the blood smear. Due to the risk of heterogeneity, both DNA-based analysis and electron microscopy on chorionic villi are recommended for prenatal examination for juvenile neuronal ceroid lipofuscinosis.     

不稳定气候条件下中上层鱼类的种群补充:瑞典四大湖泊研究

通过对瑞典维纳恩、韦特恩、梅拉伦和哈马伦四大湖泊中上层鱼类种群生物学的研究得出,适宜区域里鱼苗在生长季节尽早孵化,并在冬季到来之前达到一定的体长,对鱼苗的成活是至关重要的.一般认为,这不仅是为了适应春季浮游动植物的生长,同时也是为了避免被捕食.相对于秋季产卵的欧白鲑(Coregonus albula)来说,通过对春季产卵的胡瓜鱼(Osmerus eperlanus)和梭鲈(Stizostedion lucioperca)的研究,我们更容易得出上述的结论.冰融之后欧白鲑苗的迅速孵化对鱼类的种群生长较为有利.在贫营养性、掠食性鱼类很少的湖泊中,冰融化之后水温的快速回升对种群的补充较为有利,而在富营养性的湖泊中,由于存在被捕食的压力,情况就大不一样了.研究结果表明秋季产卵群体较难适应全球变暖的趋势,不同年份鱼类在年种群生长强度方面存在着较大的差异,这可用生活史来予以解释.     

Body stalk anomaly associated with maternal cocaine abuse

A case of body stalk anomaly diagnosed prenatally by ultrasound during the 24th week of pregnancy in a cocaine abusing mother is presented. Accurate visualization of the fetal organs was difficult due to the severe oligohydramnios caused by premature rupture of membranes, probably related to the cocaine use. The sonographic findings were an omphalocoele, fetal attachment to the placenta, kyphoscoliosis, and absence of a floating umbilical cord. The prenatal diagnosis of the syndrome and the possible relationship with cocaine abuse are discussed.     

A spatially explicit backcasting approach for sustainable land-use planning

General backcasting as a decision support and planning method starts from desired future states and simulates developments backwards until reaching the present state. Development pathways that reveal steps to be taken to reach a certain future state, and milestones that serve as interim goals, are created during the process. Backcasting has hitherto only been applied in workshops or as a theoretical framework and no spatially explicit backcasting model has previously been established. This paper presents the development of a spatially explicit backcasting model. The proposed model first creates a future scenario utilizing an agent-based model and then simulates backwards. It is implemented using the programming language Python. The model has been applied to a case study for sustainable land-use planning in Salzburg, Austria. The results of the model run show a successful backcasting of land-use classes from a future state back to the present, in 10 year time steps.